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Application of extractive of adinandra nitida leaves to preparation of anti-inflammatory analgesia medicament

An anti-inflammatory and analgesic and extractive technology is applied to the application field of the ethyl acetate part of the leaves of Populus glabrata in the preparation of anti-inflammatory and analgesic drugs, which can solve the problems of no anti-inflammatory and analgesic effect and the like, and achieve a good therapeutic effect. , less toxic effect

Active Publication Date: 2011-10-19
玉林市中医医院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are no reports at home and abroad on the research on whether the leaves of the bright leaf poplar and its parts have anti-inflammatory and analgesic effects.

Method used

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  • Application of extractive of adinandra nitida leaves to preparation of anti-inflammatory analgesia medicament
  • Application of extractive of adinandra nitida leaves to preparation of anti-inflammatory analgesia medicament
  • Application of extractive of adinandra nitida leaves to preparation of anti-inflammatory analgesia medicament

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Example 1: The preparation method of the ethyl acetate part of poplar leaf

[0019] Pulverize the leaves of the dried poplar plant, extract 3 times with 50-60% (volume concentration) ethanol reflux by the weight of the material, filter for 3 hours each time, filter, and combine the filtrates to obtain the ethanol solution of poplar leaves . Then, the ethanol solution of poplar leaves ethanol is distilled under reduced pressure to recover ethanol, and the ethanol concentrated ointment of poplar leaves is obtained. Then, the concentrated ointment of poplar leaves of bright leaves was centrifuged at 5000 rpm for 10-15 minutes to separate it into supernatant and sediment. Extract the supernatant with an equal amount of ethyl acetate to obtain an ethyl acetate extract of Poplaria radiata leaves; extract the precipitate with 3 times the amount of ethyl acetate at room temperature, and centrifuge to obtain an ethyl acetate extract of Populus radix leaves. Finally, the extrac...

Embodiment 2

[0020] Example 2: Acute Toxicity Test of Ethyl Acetate Parts of Populus radiata Leaf

[0021] Choose 20 mice with a body weight of 18-22g, half male and half male, fasting without water for 16 hours, intragastric administration twice in one day, with an interval of 6 hours between administrations, each administration giving the maximum concentration (0.085g / ml), Immediately after administration and within 7 days after administration of the medicinal solution of the ethyl acetate part of Populus glabrata leaves with the largest volume (40ml / kg), the symptoms of poisoning and death in mice were observed and recorded. At the end of the observation period, the surviving mice were sacrificed for gross autopsy to observe visceral lesions.

[0022] Table 1 Changes in body weight of mice during the acute toxicity test of the ethyl acetate part of poplar leaves

[0023]

[0024] The maximum dosage of the ethyl acetate part of the leaves of Populus radix L. gavage twice a day was 6....

Embodiment 3

[0025] Example three: the analgesic effect of the ethyl acetate part of poplar leaf

[0026] 1. The analgesic effect of the ethyl acetate part of Populus glabra leaves on mice (hot plate method)

[0027] Set the temperature of the smart hot plate instrument to 55°C, and start the test when the temperature of the hot plate reaches the preset temperature. Select a female mouse with a body weight of 18-22g, put one on the hot plate at a time, take the time (seconds) required for the mouse to lick the hind foot phenomenon as the pain threshold of the mouse, and select the pain threshold between 5s and 30s mice for the experiment. Select 50 qualified mice therefrom, be divided into five groups at random, 10 in every group, be respectively blank control group, tramadol hydrochloride group and the high-dose group of ethyl acetate part of Populus glabrata leaf (according to the mouse acute toxicity test result, this The test set the maximum dose is about 1 / 4 of the acute toxicity te...

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Abstract

The invention belongs to the technical field of medical treatment, and in particular relates to application of an extractive of adinandra nitida leaves to the preparation of an anti-inflammatory analgesia medicament. The toxicity and the anti-inflammatory analgesia pharmacodynamics evaluation are carried out on ethyl acetate parts of the adinandra nitida leaves by taking a mice acute toxicity test as a medicine toxicity index, taking a mice hot-plate test and a rat toe mechanical stabbing pain test as analgesia pharmacodynamics indexes, taking a test on the glacial acetic acid-induced capillary permeability hyperfunction of mice peritoneal cavities, a test on the dimethylbenzene-induced swelling of mice ears and a test on the cotton balling granulation of rats as anti-inflammatory pharmacodynamics indexes. The results show that the extractive has the advantages of low toxicity and very good anti-inflammatory analgesia activity and can be applied to the preparation of anti-inflammatoryanalgesia medicaments or auxiliary medicaments.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to the application of effective components of poplar leaves, especially the application of the ethyl acetate part of poplar leaves in the preparation of anti-inflammatory and analgesic drugs. Background technique [0002] Inflammation is a defense-based reaction of living tissue to various injury factors. The clinical features are redness, swelling, heat, and pain. It is the response of inflammatory factors to stimulate living tissue with vascular system to local damage. The pathological process is generally divided into early capillary hyperpermeability, exudation of vascular contents, edema, and late connective tissue hyperplasia and granuloma formation; pain refers to the somatic sensation associated with tissue damage and potential tissue damage. Or when injured, the injured part releases pain-causing substances such as bradykinin, histamine, serotonin, PG, etc., which stimulat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/82A61P29/00A61K127/00
Inventor 刘元李振麟宋志钊李星宇兰太进张宁宁文志云韦韡
Owner 玉林市中医医院
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