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Method for separating and collecting microalgae by using magnetic medium

A magnetic medium and magnetic separation technology, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of complex material synthesis, large influence of ionic strength, large amount of consumption, etc., and achieve simple synthesis steps and reduce The effect of production cost and short mixing time

Inactive Publication Date: 2012-11-28
INST OF PROCESS ENG CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Gao and Peng et al. successfully separated harmful microalgae Microcystis aeruginosa from water by using a composite of montmorillonite and copper-iron oxide. The magnetic separation medium can be reused, but the synthesis of the magnetic composite is cumbersome. And the amount is relatively large (Gao Z.W., Peng X.J., Zhang H.M., Luan Z.K., Fan B.Desalination, 2009, 247, 337); Liu and Li et al. used chitosan-modified magnetic polymers as magnetic separation media to successfully remove fresh water Algal blooms in the environment, but the synthesis of the magnetic separation medium is relatively complicated, the magnetic separation medium cannot be reused, and is greatly affected by the ionic strength (Liu D., Li F.T., Zhang B.R.Water Sci.Technol.2009, 59, 1085)
It can be seen that the separation and collection of microalgae using existing magnetic materials requires complex material synthesis, and often requires modification or coating of polymers to couple with algal cells, which greatly increases the cost. Therefore, many It is difficult to apply on a large scale, and its separation of microalgae in the culture solution system has not been verified by experiments

Method used

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  • Method for separating and collecting microalgae by using magnetic medium
  • Method for separating and collecting microalgae by using magnetic medium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Separate and collect the botrytis in the botrytis culture fluid with the method of the present invention, its step is as follows:

[0032] Fe will be prepared 3 o 4 Nanoparticles are dispersed in deionized water to form a stable suspension, prepared into a magnetic separation medium with a concentration of 2% (w / v), and then put into a refrigerator for further storage. The botrytis culture solution obtained by cultivating is placed in a stirred reactor, the temperature in the adjusted stirred reactor is 20° C., the pH value is 8, and a magnetic separation medium is added at a rotating speed of 100 rpm. The consumption of the magnetic separation medium is 0.04% (w / v), the stirring time was 1 minute; a permanent magnet with a magnetic field strength of 1000 Oersted was selected to be placed on the bottom of the reactor, and the botrytis was magnetically separated, left to stand for 1 minute, the supernatant was removed, and the botrytis obtained by separation was collec...

Embodiment 2

[0034] Separate and collect the botrytis in the botrytis culture fluid with the method of the present invention, its step is as follows:

[0035] Fe will be prepared 3 o 4The nanoparticles are dispersed in deionized water to form a stable suspension, prepared into a magnetic separation medium with a concentration of 1% (w / v), and then put into a refrigerator for further storage. Put the botrytis culture solution obtained by cultivating into a stirred reactor, adjust the temperature in the stirred reactor to be 30°C, and the pH value is 10, add a magnetic separation medium at a rotating speed of 300rpm, and the consumption of the magnetic separation medium is 0.02% (w / v), the stirring time was 2 minutes; a permanent magnet with a magnetic field strength of 2000 Oersted was selected to be placed on the bottom of the reactor, and the botrytis was magnetically separated, left to stand for 2 minutes, the supernatant was removed, and the botrytis obtained by separation was collect...

Embodiment 3

[0037] Separate and collect the botrytis in the botrytis culture fluid with the method of the present invention, its step is as follows:

[0038] Fe will be prepared 3 o 4 The nanoparticles are dispersed in deionized water to form a stable suspension, prepared into a magnetic separation medium with a concentration of 5% (w / v), and then put into a refrigerator for further storage. The botrytis culture solution obtained by cultivating is placed in a stirred reactor, the temperature in the stirred reactor is adjusted to be 25° C., the pH value is 9, and a magnetic separation medium is added at a rotating speed of 200 rpm. The consumption of the magnetic separation medium is 0.01% (w / v), the stirring time was 3 minutes; a permanent magnet with a magnetic field strength of 3000 Oersted was selected to be placed on the bottom of the reactor, and the botrytis was magnetically separated, left to stand for 3 minutes, and finally the supernatant was removed, and the grapes obtained by...

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Abstract

The invention belongs to the field of microalgae separation, and in particular relates to a method for separating and collecting microalgae by using a magnetic medium. The method comprises the following steps: (1) preparing Fe3O4 nano particles and dispersing the Fe3O4 nano particles so as to form a stable suspension; (2) regulating the pH value of a microalgae culture liquid to 8-10; (3) adding a magnetic separation medium obtained in the step (1) and stirring, wherein the use amount of the magnetic separation medium is 0.01-0.04% (w / v); and (4) carrying out magnetic separation on the microalgae obtained in the step (3), and collecting the microalgae obtained by separation. According to the invention, the method using the magnetic separation medium has simple synthesis steps and is easy to amplify; the use amount of the magnetic separation medium is less; and stirring time is short, efficiency is high, separation operation process is simple, and the magnetic separation medium can be repeatedly used, thereby being beneficial to reduction of production cost.

Description

technical field [0001] The invention belongs to the field of separation of microalgae, in particular, the invention relates to a method for separating and collecting microalgae by using a magnetic medium. Background technique [0002] Microalgae cultivation has been widely used in the fields of aquaculture, food, pharmaceuticals, and energy. However, the separation and collection of microalgae has become one of the main factors restricting the large-scale expansion of microalgae cultivation. The current separation and collection methods of microalgae mainly include: centrifugation, filtration, flocculation, and air flotation. The centrifugal method requires expensive investment in fixed assets (centrifuge) and high energy consumption; the efficiency of the filtration method is low, the membrane is easily blocked, and the operation is difficult; the separation and collection of microalgae using the flocculation method requires the use of various magnetic separation media, and...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/12C12R1/89
Inventor 刘春朝徐玲王锋郭晨
Owner INST OF PROCESS ENG CHINESE ACAD OF SCI