Method for manufacturing a porous three-dimensional support using powder from animal tissue, and porous three-dimensional support manufactured by same

An animal tissue and powder technology, applied in tissue regeneration, prosthesis, drug delivery, etc., can solve the problem that the size, porosity, shape and structure of the scaffold are easily limited, and it is not disclosed to make three-dimensional structures with various sizes, shapes and structures. stents and other problems, to achieve good biocompatibility, good clinical practicability, and the effect of enhancing regeneration

Active Publication Date: 2011-11-09
AJOU UNIV IND ACADEMIC COOP FOUND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since the known technique is to manufacture a porous three-dimensional scaffold by decellularizing natural tissue, it can reduce possible immune rejection, however, this has a disadvantage: since it uses its own natural tissue, the size, porosity, and , shape and structure are easily restricted
[0019] However, the above method has a problem: since the extracellular matrix used is obtained by decellularizing the natural tissue itself, the size, porosity, shape and structure of the scaffold are easily limited.
Therefore, it is difficult to apply the above method to commercial purposes as well as medical use
U.S. Patent No. 4,656,137 only discloses a method of using cartilage powder to treat wounds, but does not disclose at all how to use cartilage powder to make three-dimensional scaffolds with various sizes, shapes and structures

Method used

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  • Method for manufacturing a porous three-dimensional support using powder from animal tissue, and porous three-dimensional support manufactured by same
  • Method for manufacturing a porous three-dimensional support using powder from animal tissue, and porous three-dimensional support manufactured by same
  • Method for manufacturing a porous three-dimensional support using powder from animal tissue, and porous three-dimensional support manufactured by same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0066] Embodiment 1: Pulverize porcine cartilage

[0067] The washed cartilage slices were pulverized to reduce their size to about 2×2 mm by a well-known and well-used commercial pulverizer (Hood Mixer HMF-505, Hanil Co., Ltd., Korea) by those skilled in the art. The crushed cartilage slices were freeze-dried, and then the freeze-dried cartilage slices were ground into a powder with a size of about 10 μm by a freeze mill (JAI, JFC-300, Japan).

[0068] 1-1. Morphological analysis of porcine cartilage powder

[0069] The morphology of porcine cartilage powder was analyzed by scanning electron microscope. The porcine cartilage powder prepared in Example 1 was treated with 2.5% glutaraldehyde for about 1 hour, and then washed with phosphate buffer solution. The sample was dehydrated and dried, and then observed with a microscope (JEOL, JSM-6380, Japan; 20KV) to measure the size and shape of the powder. After observation, the size of the powder is about 10 μm ( figure 1 (...

Embodiment 2

[0070] Example 2: Decellularization and properties of porcine cartilage powder

[0071] 2-1. Decellularization of porcine cartilage powder

[0072] In order to remove chondrocytes and genetic components to obtain pure extracellular matrix, decellularization will be performed as follows.

[0073] The porcine cartilage powder prepared in Example 1 was added to 1 liter of 0.1% sodium dodecyl sulfate (sodium dodecyl sulfate, SDS, Bio-Rad, the U.S.) (porcine cartilage powder of every 10 g), and stirred at 100 rpm for 24 Hour. After the SDS treatment, the product was washed with triple distilled water for 5 times at 100 rpm for 30 minutes.

[0074] In order to allow the cartilage powder to settle to replace the rinsing solution, the cartilage powder was spun at 10,000 rpm in an ultracentrifuge (US-21 SMT, Vision, Korea) for 1 hour.

[0075] 200ml of 200U / ml deoxyribonuclease (Sigma, USA) was added to the cartilage powder and stirred at 100rpm at 37C for 24 hours. The product...

Embodiment 3

[0082] Example 3: Using decellularized porcine cartilage powder to manufacture a three-dimensional scaffold and explain its characteristics

[0083] 3-1. Fabrication of porous three-dimensional scaffold

[0084] The decellularized cartilage powder prepared by the same procedure as in Example 2-1 was uniformly mixed with sodium chloride (with a crystal size of 250 to 350 μm) at a ratio of 1:9. Then, a solution prepared by 100 mM EDC (N-(3-dimethylaminopropyl)-N-ethylcarbodiimide hydrochloride) (Sigma, USA) and triple distilled water was added to the mixed solution at a ratio of 10%.

[0085] The EDC solution is uniformly mixed with the mixed solution of the cartilage powder and sodium chloride. Then the above mixed solution was poured into a self-made cemented carbide mold, and molded by 1000 psi pressure casting to make a disc-shaped product. The casting mold includes a fixed component and a moving component, and forms a three-dimensional support in the fixed component a...

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Abstract

The present invention provides a method for manufacturing a porous three-dimensional support using powder from animal tissue, comprising the steps of making a powder from animal-derived tissue, decellularizing the powder before, after, or simultaneously with the step of making the powder from the animal-derived tissue, and forming the decelluralized powder from the animal-derived tissue into a porous three-dimensional support through a particle leaching method.

Description

technical field [0001] The present invention relates to a method for manufacturing a porous three-dimensional scaffold and the porous three-dimensional scaffold made by the method, especially a method for manufacturing a porous three-dimensional scaffold by using powder from animal-derived tissues and the porous three-dimensional scaffold made by the method , in which powders from animal-derived tissues are made into porous three-dimensional scaffolds with various sizes, porosities, shapes and structures according to their purpose and therapeutic use by particle filtration. Background technique [0002] Articular chondrocytes are medial-derived cells present only in cartilage. Cartilage is an avascular tissue composed of an extracellular matrix produced from chondrocytes. It does not cause inflammation and does not regenerate itself when damaged. Once the cartilage is damaged, its ability to regenerate itself is quite limited, which will eventually lead to osteoarthritis, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/38
CPCA61L2400/08A61L27/3612A61L2430/06A61L27/3683A61L27/3817A61L27/56A61L2430/40A61L27/3852A61L27/3654A61L27/3604A61L2400/18A61L27/00A61L27/36A61L27/38A61F2/00
Inventor 闵炳显张志旭
Owner AJOU UNIV IND ACADEMIC COOP FOUND
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