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Plant cell microspheres and use thereof

A plant cell and microsphere technology, applied to plant cell microspheres and their application fields, can solve the problems of lack of intelligence, environmental pollution, and high production line cost, and achieve the effects of easy expansion and upgrade, environmental gain, and emission reduction.

Active Publication Date: 2013-07-10
济南菲特生态环保工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Traditional microspheres are made by physical and chemical methods, and there are many shortcomings: (1) lack of intelligence and single performance; (2) cannot meet the standards of green food; (3) cannot meet the requirements of green and natural cosmetics, Standards for green and natural biomedical adjuvants; (4) Material production and microsphere manufacturing process are complex; (5) Smart microsphere production is difficult; (6) Microsphere functional modification directly involves changes in production lines, which will upgrade production lines The cost is high; (7) The production of microsphere materials and the manufacturing process of microspheres consume high energy and high pollution; (8) Some microspheres are not easy to degrade, causing environmental pollution; (9) The production process of microspheres itself has no environmental benefits

Method used

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  • Plant cell microspheres and use thereof
  • Plant cell microspheres and use thereof
  • Plant cell microspheres and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] The plant cell microsphere is a microsphere processed and manufactured on the basis of plant protoplasts, plant cells, plant pollen or pollen, and the plant cell microspheres derived from protoplasts contain a matrix, a matrix channel, a cavity, an inner sphere and an outer part ; Cell-derived plant cell microspheres contain an outer wall, a matrix, a matrix channel, a cavity, an inner sphere and an outer part; a sporopollen-derived plant cell microsphere contains an outer wall, an inner wall, a matrix, a matrix channel, a cavity, an inner sphere and an outer part pieces.

[0048] Plant cell microspheres are produced by prokaryotic or eukaryotic plant cells or sporopollen as raw materials, with plant cells as the main body of the microsphere structure, which can include protoplasts, cell walls (walled microspheres), sporopollenia (sporopollen microspheres or pollen microspheres), all of the internal and external accessories, or a combination of one or more of them.

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Embodiment 2

[0051] Embodiment 2: plant cell microspheres of protoplast origin:

[0052] Plant cell microsphere protoplast refers to the remaining cell part of plant cells after the cell wall is removed, and its structure includes cell membrane, nucleus (or nuclear region of prokaryotic cells), organelles (or non-membrane-coated functional regions corresponding to prokaryotic cells) , and the cytoplasm. Its structure and components can change its existence and properties through the genetic improvement, modification, mutagenesis, selection of developmental stage, treatment and processing involved in the production process of the plant cell microspheres.

[0053] Prokaryotic or eukaryotic plant cells, especially somatic cells, are used as solid or liquid medium for fermentation, such as: B5, MS, NLN and other medium. Cultivation temperature: 0-80°C. Shaking speed: 1-500rpm. The culture medium contains N, P, K, Fe, Ca and other macroelements, Mn and other trace elements, vitamins and othe...

Embodiment 3

[0057] Example 3: Cell-derived plant cell microspheres:

[0058] Cells of prokaryotic or eukaryotic plants, especially somatic cells, are cultured in solid or liquid medium (for example: B5, MS, NLN, etc.) for fermentation. Cultivation temperature: 0-80°C. Shaking speed: 1-500rpm. The medium contains N, P, K, Fe, Ca and other macroelements, Mn and other trace elements, vitamins and other organic elements and sugar, etc., pH 5-8. The above items, as well as the cultivation conditions such as light time, light intensity and wavelength, are determined according to the requirements of the species and the shape, structure, material composition, function, color and yield of the finished microspheres.

[0059] The suspension cells produced by culture can be separated by centrifugation (100-5000rpm), filtration (50-400 mesh filter), adsorption (organic or inorganic material adsorption, affinity material adsorption, etc.) or electrophoresis separation (corresponding culture medium or...

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Abstract

The invention relates to plant cell microspheres and particularly discloses plant cell microspheres and use thereof. The plant cell microspheres are characterized by being processed and manufactured on the basis of plant protoplasts, plant cells and plant sporopollen or pollen, wherein the protoplast-derived plant cell microspheres contain matrix, matrix channels, middle cavities, internal spheres and external pendants; the cell-derived plant cell microspheres contain outer walls, matrix, matrix channels, middle cavities, internal spheres and external pendants; and the sporopollen-derived plant cell microspheres contain outer walls, inner walls, matrix, matrix channels, middle cavities, internal spheres and external pendants. The plant cell microspheres of the invention can conduct sustained-release, controlled-release and other physical and chemical functions, and can naturally degrade after being used.

Description

(1) Technical field [0001] The invention relates to a microsphere, in particular to a plant cell microsphere and its application. (2) Background technology [0002] Microsphere is a spherical and nearly spherical solid, hollow or hole, layer, gap and other conforming entities with a diameter between 1 and 1000 microns developed in the field of new materials. It has the functions of loading, unloading, filling, mechanical strength strengthening or softening. , Adhesive, curing, thermal conductivity or heat insulation, electrical conductivity, resistance or insulation, light transmission, reflection, light filtering, adsorption, filtration, sterilization, acid-base buffering, redox, replacement, slow release and controlled release of substances, etc. Function. [0003] Traditional microspheres are made by physical and chemical methods, and there are many shortcomings: (1) lack of intelligence and single performance; (2) cannot meet the standards of green food; (3) cannot meet...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01J13/02
Inventor 徐涵
Owner 济南菲特生态环保工程有限公司
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