Paddy rice endogenic nitrogen-fixing bacterium antagonistic to gibberella zeae and sclerotinia sclerotiorum and application thereof

A technology of nitrogenase and pathogenic fungi, applied in the direction of application, bacteria, biocide, etc., to achieve broad application prospects, high nitrogenase activity, and good inoculation effect

Active Publication Date: 2011-11-23
内蒙古金丰盛达农业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the bottleneck restricting the development of my country's microbial fertilizer industry is the selection of high-efficiency strains

Method used

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  • Paddy rice endogenic nitrogen-fixing bacterium antagonistic to gibberella zeae and sclerotinia sclerotiorum and application thereof
  • Paddy rice endogenic nitrogen-fixing bacterium antagonistic to gibberella zeae and sclerotinia sclerotiorum and application thereof
  • Paddy rice endogenic nitrogen-fixing bacterium antagonistic to gibberella zeae and sclerotinia sclerotiorum and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Example 1, Isolation and identification of rice endogenous nitrogen-fixing bacteria GDSD223

[0044] 1. Isolation of rice endophytic nitrogen-fixing bacteria GDSD223

[0045] The specific operation for the isolation of endogenous nitrogen-fixing bacteria in rice is as follows: take fresh rice plants (collected from Heilongjiang Province, China), first rinse them with tap water, then soak them in 70% ethanol for 1 min, sterilize the surface with 2% sodium hypochlorite for 10 min, and sterile Rinse with water 3 times. Under aseptic operation conditions, accurately weigh 10.0g of the sample, grind it into a paste in a sterile mortar, transfer and set the volume to 100ml, and continue to dilute to make serial dilution samples, respectively starting from 10 -4 、10 -5 、10 -6 Take 0.1ml of the diluted solution and spread evenly on the above-mentioned CCM medium and nitrogen-free medium plate respectively, culture upside down at 28°C, and after 3-4 days, pick a single colony...

Embodiment 2

[0065] Embodiment 2, determination of nitrogenase activity of Bacillus sp. GDSD223 CGMCC No.5036

[0066] The Bacillus sp. (Bacillus sp.) GDSD223 CGMCC No.5036 that embodiment 1 is obtained carries out nitrogenase activity assay, and concrete method is as follows: in 15 * 150mm screw-top glass tubes, add 5ml improved nitrogen fixation medium and make inclined-plane, Inoculate Bacillus sp. GDSD223 CGMCC No.5036 and culture at 28°C. Azotobacter chroococcum (Azotobacter chroococcum) ACCC11103, which is commonly used in the production of microbial fertilizers, was used as the positive control, and the blank slant without inoculation was used as the negative control, with 3 replicates. After culturing for 72 hours, replace the rubber stopper, inject acetylene gas to make the final concentration 10%, seal it with medical adhesive tape, continue culturing for 72 hours, take 100 μl of reaction gas, measure the amount of ethylene produced by gas chromatography, and calculate the nitrog...

Embodiment 3

[0069] Embodiment 3, Bacillus sp. (Bacillus sp.) GDSD223 CGMCC No.5036 antagonizes pathogenic fungus bacteriostatic rate determination

[0070]The Bacillus sp. GDSD223 CGMCC No.5036 obtained in embodiment 1 is carried out to measure the antibacterial rate of antagonizing pathogenic fungi by two-point confrontation method, and the specific operation is as follows: on the PDA plate, on two points of 2 cm away from the center The crop pathogenic fungus Gibberella zeae (or Sclerotinia sclerotiorum) and Bacillus sp. GDSD223 CGMCC No.5036 were inoculated respectively, and each screening treatment was repeated three times to inoculate only the pathogenic fungus and not A plate of Bacillus sp. GDSD223 CGMCC No.5036 was used as a control. Incubate at a constant temperature at 28°C, and measure the colony radius r of the pathogenic fungi on the confrontation plate along the direction of the tested Bacillus sp. GDSD223 CGMCC No.5036 with a millimeter scale after 15 days 1 , and the colo...

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Abstract

The invention discloses a paddy rice endogenic nitrogen-fixing bacterium antagonistic to gibberella zeae and sclerotinia sclerotiorum and application thereof. The paddy rice endogenic azotobacterium is bacillus sp. GDSD223 which is collected in the China General Microbiological Culture Collection Center with the collection number CGMCC No.5036. The bacillus sp. GDSD223 CGMCC No.5036 provided by the invention has higher nitrogen-fixing activity, can be effectively antagonistic to gibberella zeae and sclerotinia sclerotiorum, and has wide application prospect to the production of nitrogen-fixing microbial agents and biological organic fertilizers.

Description

technical field [0001] The invention relates to rice endophytic nitrogen-fixing bacteria antagonizing scab and sclerotinia and its application. Background technique [0002] Nitrogen fertilizer is one of the most important means of production in agricultural production. The production of chemical nitrogen fertilizer consumes a large amount of energy, accounting for 70% to 80% of the total production cost. In recent years, energy has become increasingly tense, and the price of coal for chemical fertilizer production in my country has soared, leading to an increase in the price of chemical nitrogen fertilizers, which is unbearable for farmers and has a strong response. In addition, chemical nitrogen fertilizers have played a huge role in increasing crop yields and ensuring food security in my country, but unreasonable nitrogen application has formed serious non-point source pollution, causing a series of serious hazards, such as the shocking cyanobacteria incident in Lake Tai...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01P3/00C12N9/02C05F11/08C05G3/00C12R1/07
Inventor 孙建光徐晶高淼胡海燕
Owner 内蒙古金丰盛达农业有限公司
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