Method for producing laccase by composite fermentation of white-rot fungus

Abstract

The invention discloses a method for producing laccase by composite fermentation of white-rot fungus. The method comprises the steps of: inoculating a culture liquid with activated composite bacteria and carrying out fermentation culture at the temperature of 25-30 DEG C for 9-12 days, wherein the composite bacteria comprise at least two of phlebia radiata, dichomitus squalens and ceriporiopsis subvermispora; and the culture liquid is composed of a liquid base culture medium and crop straw powder which is at least one of corn stalk powder, corn cob powder, wheat stalk powder and rice straw powder. In the method, the corn cob powder is used as a raw material, and the combined synergic fermentation of phlebia radiate and dichomitus squalens is adopted, so that the activity of the generated laccase stably reaches 110-120 U / mL at the 12th day. The method has the characteristics that liquid fermentation is adopted, the materials are widely available, and the process is simple; aromatic compounds or heavy metal ion induction and the like are not needed and the conditions are mild; the generated laccase has high activity and good stability; and the method is a safe, environment-friendly, low-cost and efficient production method with very promising industrial prospects.

Description

technical field [0001] The invention relates to a new method for cultivating the compound fermentation of white-rot fungus with synergistic high-yield laccase, and uses different crop stalks as fermentation substrates, including corn stalks, corn cobs, wheat stalks and straw stalks, belonging to biological engineering and microbial fermentation. Background technique [0002] Laccase (Laccase, E.C.1.10.3.2) is a kind of copper-containing polyphenol oxidase, which was first discovered from the sap of sumac tree, and later found that it also widely exists in fungi, especially in white rot fungi. White rot fungi are thought to be the only organisms capable of degrading the entire woody component. Laccase uses molecular oxygen as the final electron acceptor, can widely oxidize polyphenols, methoxyphenols, diamines and other phenolic and aromatic compounds, can oxidize phenolic substances into quinones, and can catalyze the oxidation of aromatic rings. The cleavage of branched C...

AI Technical Summary

Problems solved by technology

At present, there are few domestic reports on the industrial production of laccase, and the production of laccase is still in the stage of theoretical exploration and experimentation

Moreover, the current fermentation and cultivation methods of laccase are mainly limited to the liquid or solid fermentation culture of single bacteria, and the research is mainly aimed at optimizing the culture medium and fermentation conditions of liquid or solid fermentation culture of single bacteria, and there has not been any particularly prominent one that can be industrialized. Production Enzyme production effect

Images