Plant male fertility related protein and its coding gene and application

A technology for male fertility and related proteins, applied in the fields of plant genetic improvement, botanical equipment and methods, applications, etc., can solve the problems of long cycle, complex mechanism of action, and shortened breeding cycle.

Inactive Publication Date: 2011-11-30
PEKING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] For a long time, people have used conventional breeding methods to select sterile lines, or to transfer sterility from one variety to another. These methods cycle Long time, slow effect, unable to meet the urgent needs of production development
At present, many genes that control male sterility of plants have been cloned, but because plant sterility is affected by nuclear genes, mitochondrial genes, chloroplast genes and environmental factors, the mechanism of action is relatively complicated, and people's understanding of it is still insufficient, so It is difficult to obtain obvious results in a short period of time. Compared with male sterile breeding by conventional methods, genetic engineering male sterile has some characteristics: the breeding cycle is shortened, the fertility is relatively stable, less affected by the environment, less dependent on genotype, less environmental pollution

Method used

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  • Plant male fertility related protein and its coding gene and application
  • Plant male fertility related protein and its coding gene and application
  • Plant male fertility related protein and its coding gene and application

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Embodiment 1, the discovery of CsETR1

[0044] A new protein CsETR1 was found from Zhongnong No. 5, which consists of the amino acid residues shown in sequence 1 in the sequence listing. The coding gene of CsETR1 is named as CsETR1, and its coding region is shown in sequence 2 of the sequence listing.

Embodiment 2

[0045] Embodiment 2, the acquisition and identification of transgenic plants

[0046] In order to transfer the antisense gene of CsETR1 gene into the stamens of Arabidopsis thaliana without affecting the functions of other organs, the specific promoter AP3 of class B genes was used to link the target gene to the downstream of the AP3 promoter. Arabidopsis has a homologous gene of CsETR1 gene, so the antisense gene of CsETR1 gene can inhibit the expression of homologous gene in Arabidopsis, thereby affecting the male fertility of Arabidopsis anthers.

[0047] 1. Obtaining of transgenic plants

[0048] (1) Construction of recombinant expression vector

[0049] 1. Preparation of the promoter

[0050] Using the Arabidopsis thaliana AP3 promoter sequence on NCBI, design a pair of primers as follows:

[0051] Upstream primer: 5'-AGGGATCCAACTTGTGATTCCTTCTTAA-3';

[0052] Downstream primer: 5'-CACCATGGATTTCTTCTCTCTTTGTTTAATC-3'.

[0053] Using the genomic DNA of Arabidopsis thali...

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Abstract

The invention discloses a plant male fertility-related protein, its coding gene and application. The protein provided by the present invention is the protein of the following (a) or (b): (a) a protein composed of the amino acid sequence shown in Sequence 1 in the sequence listing; (b) the amino acid sequence of Sequence 1 is processed by one or more A protein derived from SEQ ID NO: 1 that has substitution and/or deletion and/or addition of amino acid residues and is associated with plant male fertility. By inhibiting the expression of the gene encoding the protein in the target plant, male sterile transgenic plants can be obtained. The coding gene of the protein can be used to breed various male sterile transgenic plants, and has great significance and value to agriculture.

Description

technical field [0001] The invention relates to a plant male fertility-related protein, its coding gene and application. Background technique [0002] Since the 1970s, the utilization of heterosis has made a significant contribution to rice production in my country. Facing the new and important demand of "high yield, high quality, high efficiency, safety, and ecology" put forward by my country's economic development for agricultural production, whether to further explore the application potential of heterosis has become a severe challenge for contemporary scientists. [0003] The formation of heterosis is based on the combination of two different parents. To further explore its potential on the basis of existing applications, in addition to strengthening the research on the formation mechanism of heterosis, it is also urgent to establish effective methods to create male sterility traits, so as to effectively expand the screening of hybrid combinations and the production of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/415C12N15/29C12N15/63C12N5/10C12N1/15C12N1/19C12N1/21C12N15/11A01H5/00
Inventor 王东辉白书农许智宏
Owner PEKING UNIV
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