Plant male fertility related protein and its coding gene and application
A technology for male fertility and related proteins, applied in the fields of plant genetic improvement, botanical equipment and methods, applications, etc., can solve the problems of long cycle, complex mechanism of action, and shortened breeding cycle.
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Embodiment 1
[0043] Embodiment 1, the discovery of CsETR1
[0044] A new protein CsETR1 was found from Zhongnong No. 5, which consists of the amino acid residues shown in sequence 1 in the sequence listing. The coding gene of CsETR1 is named as CsETR1, and its coding region is shown in sequence 2 of the sequence listing.
Embodiment 2
[0045] Embodiment 2, the acquisition and identification of transgenic plants
[0046] In order to transfer the antisense gene of CsETR1 gene into the stamens of Arabidopsis thaliana without affecting the functions of other organs, the specific promoter AP3 of class B genes was used to link the target gene to the downstream of the AP3 promoter. Arabidopsis has a homologous gene of CsETR1 gene, so the antisense gene of CsETR1 gene can inhibit the expression of homologous gene in Arabidopsis, thereby affecting the male fertility of Arabidopsis anthers.
[0047] 1. Obtaining of transgenic plants
[0048] (1) Construction of recombinant expression vector
[0049] 1. Preparation of the promoter
[0050] Using the Arabidopsis thaliana AP3 promoter sequence on NCBI, design a pair of primers as follows:
[0051] Upstream primer: 5'-AGGGATCCAACTTGTGATTCCTTCTTAA-3';
[0052] Downstream primer: 5'-CACCATGGATTTCTTCTCTCTTTGTTTAATC-3'.
[0053] Using the genomic DNA of Arabidopsis thali...
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