Application of Schizophyllan in Controlling Tobacco Mosaic Virus Disease

A technology of Schizophyllum polysaccharide and Schizophyllum, which is applied in the directions of application, disinfectant, chemical used for biological control, etc., can solve the problem that the application of anti-plant virus has not yet been reported, and achieves low cost, simple preparation method, good control effect

Inactive Publication Date: 2011-12-07
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] After retrieval, schizophyllan is mainly used in the field of human medicine, and its application in anti-plant virus has not been reported yet.

Method used

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  • Application of Schizophyllan in Controlling Tobacco Mosaic Virus Disease

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] The cultivation of embodiment 1 schizophyllum and the preparation of mycelium powder

[0022] Proceed as follows:

[0023] (1) activation of the mother species: the mother species of the Schizophyllum is transferred to the PDA solid medium (the composition and weight percentages of the PDA solid medium are: potato starch 20%, glucose 20%, agar 20%, and all the other are water ) and cultured at 28°C for 10 days;

[0024] (2) Preparation of the primary strain: the plate mycelium obtained in step (1) is cut into small pieces less than 1mm, and the Schizophyllum mycelium is transferred to a 100ml liquid fermentation tank according to the inoculation amount of 0.5g mycelium / 100ml Culture medium (the composition and weight percent of liquid fermentation medium are: soluble starch 4%, glucose 4%, wheat flour 2%, corn flour 2%, yeast extract 0.3%, soybean flour 0.5%, peptone 0.2%, KH 2 PO 4 1%, MgSO 4 0.5%, VB 1 0.01%, and the rest is water) in a 500ml shake flask, at 28°C...

Embodiment 2

[0026] The preparation of embodiment 2 schizophyllan extract

[0027] Proceed as follows:

[0028] (1) Add 10 g of Schizophyllum mycelium powder to 100 mL of hot water at 98° C., extract in a water bath at 100° C. for 8 hours, and filter to obtain filtrate and filter residue;

[0029] (2) Add the filter residue obtained in step (1) into 100 mL of hot water at 98°C, extract in a water bath at 100°C for 8 hours, filter, and collect the filtrate;

[0030] (3) The 2 filtrates of steps (1) and (2) were combined, concentrated in vacuo to 5 mL, fully mixed with 25 mL volume of 95% ethanol, left to stand overnight at 4 ° C, centrifuged at 5000 rpm for 10 min, poured off the supernatant, and the precipitate was White fibrous, the precipitate was washed twice with 75% ethanol, dried at 60°C until constant weight, weighing 1.12g.

[0031] Determination of polysaccharide content: enone-sulfuric acid assay

[0032] Preparation of reagents:

[0033] Glucose standard solution: 100ug / mL (...

Embodiment 3

[0040] The preparation of embodiment 3 schizophyllan extract

[0041] Proceed as follows:

[0042] (1) Add 12 g of Schizophyllum mycelium powder into 180 mL of hot water at 95° C., extract in a water bath at 100° C. for 9 hours, and filter to obtain filtrate and filter residue;

[0043] (2) Add the filter residue obtained in step (1) into 180 mL of hot water at 95°C, extract in a water bath at 100°C for 9 hours, filter, and collect the filtrate;

[0044] (3) Combine the two filtrates of steps (1) and (2), concentrate in vacuo to 6 mL, mix the filtrate with 30 mL of 95% ethanol, let it stand overnight at 4 ° C, centrifuge at 5000 rpm for 10 min, pour off the supernatant, and the precipitate is white The fibrous crude polysaccharide was washed twice with 75% ethanol, dried at 60°C until constant weight, and weighed 1.41g. The polysaccharide content in the sample was calculated to be 0.452 g by the standard curve prepared by the enone-sulfuric acid assay.

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Abstract

The invention discloses a schizophyllan extract and a preparation method thereof in the field of biological pesticides. The method comprises the following steps of: adding schizophyllum commune hypha powder into hot water in a ratio, performing extraction for 8 to 10 hours in a water bath of 100 DEG C, filtering the extract by using gauze, extracting the obtained filter residue again by using thesame method, combining the filtrate of two times, performing vacuum concentration, mixing the concentrate and 95 percent ethanol in an amount which is 5 times volume of the concentrate, standing the mixture over night at the temperature of 4 DEG C, centrifuging, washing the sediment for 2 times by using ethanol, drying the sediment at the temperature of 60 DEG C, and thus obtaining the schizophyllan extract. The invention also discloses application of the schizophyllan extract in prevention and control of tobacco mosaic virus diseases. The schizophyllan extract has good prevention and controleffect on tobacco mosaic viruses, the tobacco mosaic virus inhibiting rate can reach over 70 percent, and a new path is provided for prevention and control of the tobacco mosaic virus diseases; the schizophyllan extract is a natural extract, and is harmful to the human being and the environment; and moreover, the preparation method is simple and low in cost.

Description

technical field [0001] The invention belongs to the field of plant disease prevention and control, and in particular relates to the application of schizophyllan extract in the prevention and treatment of tobacco mosaic virus disease. Background technique [0002] Tobacco mosaic virus (TMV) can infect more than 300 species of plants such as Solanaceae, Cucurbitaceae, and Brassicaceae, and is one of the most harmful viruses. The main method of preventing and treating tobacco mosaic virus disease is to cultivate disease-resistant varieties and chemical agents. The breeding methods are limited due to the lack of sources of disease-resistant genes. The effect of chemical agents on the prevention and treatment of tobacco mosaic virus disease is not only unsatisfactory, but also causes environmental pollution. , so its application is also limited. Therefore, botanical pesticides with less environmental damage and high antiviral activity have become a research hotspot. [0003] Sc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N43/16A01P1/00
Inventor 周莹李兴红燕继晔严红
Owner BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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