A method for extracting cantharidin with compound enzyme

A cantharidin and compound enzyme technology, which is applied in the fields of drug combination, sexual diseases, organic chemistry, etc., to achieve the effect of convenient follow-up processing, less product impurities, and increased white blood cells

Inactive Publication Date: 2011-12-07
BIOCHEM ENG COLLEGE OF BEIJING UNION UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The novel method of preparing cantharidin of the present invention has not yet been reported at home and abroad

Method used

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  • A method for extracting cantharidin with compound enzyme
  • A method for extracting cantharidin with compound enzyme
  • A method for extracting cantharidin with compound enzyme

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] (1) Take the southern big mylabris ( Mylabris phalerata Pallas ) 500g of medicinal materials, pulverized and passed through a 10-20 mesh sieve.

[0032] (2) Soak with water, add 0.8g of cellulase and 1.6g of pectinase per 500g of medicinal materials.

[0033] (3) Adjust the pH to 5.0 with HCl, enzymatically hydrolyze at 50°C for 2.5 hours, and then boil for 3 minutes to inactivate the enzyme.

[0034] (4) Extract the enzymatic hydrolysis product with chloroform-absolute ethanol-concentrated hydrochloric acid (volume ratio 1.5:1:1) in a 66°C water bath for 24 hours, filter the extraction product with hot suction, and add chloroform- Absolute ethanol-concentrated hydrochloric acid (volume ratio 1:2:1) leaching, hot suction filtration.

[0035] (5) The two filtrates were combined and evaporated to dryness, the obtained solid was dissolved in water-chloroform (volume ratio 2:1), and the lower organic phase was extracted.

[0036] (6) Rotate the lower organic phase to dry...

Embodiment 2

[0039] (1) Take 500g of Chinese mylabris medicinal material, crush it, and pass it through a 10-20 mesh sieve.

[0040] (2) Soak with water, add 1g of cellulase and 1.8g of pectinase per 500g of medicinal materials.

[0041] (3) Adjust the pH to 5.8 with HCl, enzymatically hydrolyze at 48°C for 3 hours, and then boil for 4 minutes to inactivate the enzyme.

[0042] (4) Extract the enzymatic hydrolysis product with chloroform-absolute ethanol-concentrated hydrochloric acid (volume ratio 1.5:1.5:1) in a water bath at 57°C for 23 hours, filter the extracted product with hot suction, and add chloroform- Absolute ethanol-concentrated hydrochloric acid (volume ratio 1:2:1.5) leaching, hot suction filtration.

[0043] (5) The two filtrates were combined and evaporated to dryness, the obtained solid was dissolved in water-chloroform (volume ratio 2:1.5), and the lower organic phase was extracted.

[0044] (6) Rotate the lower organic phase to dryness, add absolute ethanol-petroleum ...

Embodiment 3

[0047] (1) Take yellow and black mylabris ( M. cichorii Linnaeus ) 500g of medicinal materials, pulverized and passed through a 10-20 mesh sieve.

[0048] (2) Soak with water, add 1.5g of cellulase and 2.0g of pectinase per 500g of medicinal materials.

[0049] (3) Adjust the pH to 6.0 with HCl, enzymatically hydrolyze at 45°C for 2.5 hours, and then boil for 2 minutes to inactivate the enzyme.

[0050] (4) Extract the enzymatic hydrolysis product with chloroform-absolute ethanol-concentrated hydrochloric acid (volume ratio 1.5:1:1) in a 70°C water bath for 25 hours, filter the extracted product with hot suction, and add chloroform- Absolute ethanol-concentrated hydrochloric acid (volume ratio 1:2:1.5) leaching, hot suction filtration.

[0051] (5) The two filtrates were combined and evaporated to dryness, the obtained solid was dissolved in water-chloroform (volume ratio 2:1.5), and the lower organic phase was extracted.

[0052] (6) Rotate the lower organic phase to dryn...

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Abstract

The invention relates to a method for extracting cantharidin from compound enzyme. The method comprises the following steps of: performing enzymolysis on a mylabris medicinal material with cellulase and pectic enzyme; leaching a hydrolysate with a mixed liquid with the volume ratio of chloroform to absolute ethanol to concentrated hydrochloric acid of (1-2):(1-2):(1-2); performing suction filtration on a leached product in hot state and adding chloroform-absolute ethanol-concentrated hydrochloric acid in the volume ratio of (1-2):(1-2):(1-2) for leaching; combining filtrates obtained in two times and performing rotary steaming; dissolving the obtained solid by using a mixed liquid in the volume ratio of water to chloroform of (1-2):(1-2); extracting a lower layer organic phase and performing rotary steaming on the lower layer organic phase; adding a mixed liquor in the volume ratio of absolute ethanol to petroleum ether of (1-2):(1-2) into the obtained solid for dissolving; and recrystallizing with acetone to obtain cantharidin. Compared with the conventional extraction method, the method has the advantages of high extracting speed, low cost, time saving, labor saving, high extraction rate, lower impurity content, easiness for purification and the like, and is suitable for large-scale industrial production.

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicine pharmacy or raw material processing, and in particular relates to a method for extracting cantharidin by a compound enzyme method. Background technique [0002] Mylabris, including insects of the family Mylaridae, the southern mylabris (Perthus spp.) Mylabris phalerata Pallas or yellow and black mylabris (eye spot phlox) M. cichorii Linnaeus , is a kind of traditional Chinese medicinal material with a wide range of uses. Clinical and pharmacological studies have shown that in addition to anti-cancer, mylabris has various activities such as anti-virus, anti-fungal, aphrodisiac, and white blood cell increase. Cantharidin contains substances such as cantharidin, fat, wax, formic acid and pigment, among which cantharidin is the main active ingredient. As an anticancer drug with high application value, cantharidin has attracted more and more attention from the world. [0003] The extract...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D493/18A61P31/12A61P31/04A61P31/10A61P15/00
Inventor 葛喜珍杜晓霞田平芳林强王路
Owner BIOCHEM ENG COLLEGE OF BEIJING UNION UNIV
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