A kind of Saccharomyces cerevisiae strain and the method for using it to prepare blackberry fruit wine
A technology of Saccharomyces cerevisiae strain and berry wine, which is applied in the preparation of alcoholic beverages, microorganism-based methods, biochemical equipment and methods, etc., can solve the problems of insufficient fruit flavor, large loss of vitamins, turbidity or precipitation, etc. The growth of bad bacteria, the effect of strong targeting and better flavor
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Embodiment 1
[0029] Saccharomyces cerevisiae strain CNBG001 was inoculated on wort slant medium with a soluble solid content of 5%, cultured at 25°C for 1 day, taken out, refrigerated at 5°C, and transferred every 30 days.
[0030] Using wort natural medium, adjust Brix (soluble solids content) to 5%, and sterilize at 121°C for 20 min. Under sterile conditions, a ring of yeast was taken from the slant medium, added to the liquid seed medium, and incubated at 25°C for 18 h.
[0031] Take 5 kg of blackberry fruit, sort and remove spoiled and rotten fruit, heat to 60°C for 10 minutes to soften, then crush, add sucrose to adjust the sugar content to 23.3, when the temperature drops below 30°C, add 20 mL of liquid seed medium with activated strains, Cultured at 25°C for 7 days. When the liquid level is calm, pour the tank, filter to remove the surface dross, and obtain about 3.2 kg of fermented liquid with an alcohol content of 13.1% vol. Include SO in 2 , with a dosage of 50 mg / kg, and cult...
Embodiment 2
[0033] The wort natural culture medium was used, Brix was adjusted to 5%, and sterilized at 121°C for 20 min. Under sterile conditions, take a ring of yeast from the slant medium in the above example, add it to the liquid seed medium, and incubate at 25°C for 18 h.
[0034] Take 50 kg of blackberry fruit, sort and remove rotten fruit, heat to 60°C to soften for 10 minutes, then crush, add sucrose to adjust the sugar content to 23.5, when the temperature drops below 30°C, add 200mL of liquid seed medium with activated strains, 25 ℃ static culture for 7 days. After the liquid level is calm, pour the tank, filter to remove the surface scum, and obtain about 33.4 kg of fermented liquid with an alcohol content of 12.5% vol. Include SO in 2, with a dosage of 50 mg / kg, and cultured statically at 18°C for 14 days. After the liquid level is calm, pour the jar and filter to obtain the fermented liquid. Add PVPP in an amount of 50.0 g, mix well, let stand for 7 days, and filter t...
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