A kind of Saccharomyces cerevisiae strain and the method for using it to prepare blackberry fruit wine
A technology of Saccharomyces cerevisiae strain and berry wine, which is applied in the preparation of alcoholic beverages, microorganism-based methods, biochemical equipment and methods, etc., can solve the problems of insufficient fruit flavor, large loss of vitamins, turbidity or precipitation, etc. The growth of bad bacteria, the effect of strong targeting and better flavor
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[0028] Example 1
[0029] Saccharomyces cerevisiae strain CNBG001 was inoculated on a wort slant medium with a soluble solid content of 5%, incubated at 25°C for 1 day, taken out, refrigerated at 5°C, and transferred every 30 days.
[0030] Using natural wort medium, adjust Brix (soluble solid content) to 5%, sterilize at 121°C for 20 min. Under aseptic conditions, take a ring of yeast from the slant medium, add it to the liquid seed medium, and incubate at 25°C for 18 h.
[0031] Take 5 kg of blackberry fruits, select to remove spoilage and rotten fruits, heat to 60°C to soften them for 10 minutes, then break them, add sucrose to adjust the sugar content to 23.3, and when the temperature drops below 30°C, add 20 mL of liquid seed medium with activated strains, Incubate at 25°C for 7 days. When the liquid level is calm, pour the tank, filter to remove the surface scum, and obtain about 3.2 kg of fermentation broth with an alcohol content of 13.1% vol. Add SO to it 2 ,Dosage 50 mg...
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[0032] Example 2
[0033] Use natural wort medium, adjust Brix to 5%, and sterilize at 121°C for 20 min. Under aseptic conditions, take a ring of yeast from the slant medium in the above example, add it to the liquid seed medium, and incubate at 25°C for 18 h.
[0034] Take 50 kg of blackberry fruits, select to remove spoilage and rotten fruits, heat to 60°C to soften them for 10 minutes, then break them, add sucrose to adjust the sugar content to 23.5, wait for the temperature to drop below 30°C, add 200mL of liquid seed culture medium with activated bacteria, 25 Cultivation at ℃ for 7 days. When the liquid level is calm, pour the tank and filter to remove the surface scum to obtain about 33.4kg of fermentation broth with an alcohol content of 12.5% vol. Add SO to it 2 ,Dosage 50 mg / kg, static culture at 18℃ for 14 days. After the liquid level is calm, pour the tank and filter to obtain the fermentation broth. PVPP was added, the addition amount was 50.0g, and after mixing...
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