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Extract containing beta-1,3-D-glucan and use thereof

A technology of extract and dextran, which is applied in the direction of medical preparations containing active ingredients, applications, microorganisms, etc., can solve the problems of large molecular weight and low purity, and achieve the effects of inhibiting tumors, promoting metabolism, and regulating blood sugar

Active Publication Date: 2013-09-11
SHANGHAI KEAI BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0022] Chinese invention patent application 200610108522.9 discloses an extract of Hydrangea MH-3 bacterial strain and its preparation method and application. After extracting the fruiting body of Hydrangea with hot water, cold alkaline water and hot alkaline water respectively, the obtained Hydrangea β-1,3 glucan, but its purity is low and its molecular weight is relatively large, around 2 million

Method used

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  • Extract containing beta-1,3-D-glucan and use thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0038] Crush 10kg of dried Hydrangea to 80 mesh, add it to a 200L autoclave, add 50kg of phosphate buffer solution (pH value is 7.3, specifically prepared as disodium hydrogen phosphate 1.97 g and potassium dihydrogen phosphate 0.22 g, add water to dissolve into 1000 mL, adjust the pH value to 7.3), stir evenly, heat to 121°C, keep warm for 30 minutes, sterilize the material, then quickly cool the material to 30°C, add 100g β-1,3 glucosidase , Stir evenly, hydrolyze for 3 hours, hydrolyze the macromolecule β-1,3 glucan into β-1,3 glucan with a specific molecular weight, and vacuum-dry the hydrolyzed slurry at 45°C to control the moisture content below 12% , to obtain the extract, which contains 45% of β-1,3-D-glucan with a molecular weight of 200,000-500,000 Daltons. The extract is ultra-low temperature pulverized into a 180-mesh ultra-fine powder, which is recorded as: KA-231.

Embodiment 2

[0040] Crush 10kg of dried Hydrangea to 80 mesh, add it to a 200L autoclave, add 50kg of phosphate buffer (pH value is 7.3, specifically prepared as disodium hydrogen phosphate 1.97 g and potassium dihydrogen phosphate 0.22 g, add water to dissolve into 1000 mL, adjust the pH value to 7.3), stir evenly, heat to 121°C, keep warm for 30 minutes, sterilize the material, then quickly cool the material to 30°C, add 100g β-1,3 glucoside Enzyme, stir evenly, hydrolyze for 3 hours, hydrolyze macromolecular β-1,3 glucan into β-1,3 glucan with specific molecular weight, add 70kg of water, boil for 2 hours, filter, and add the filter residue 100kg of water, boiled and extracted for 2 hours, filtered. The combined filtrate was concentrated in vacuum at 70°C to 50kg, added ethanol until the concentration of ethanol reached 65% (v / v), stirred evenly, stood for 2 hours, centrifuged at 3,000rpm for 5 minutes, discarded the precipitate, and continued to the supernatant Add ethanol until the e...

Embodiment 3

[0042] The KA-232 obtained in Example 2 was separated and purified by Sephadex G-100 gel chromatography, the eluent was 0.1M NaCl solution, the elution peak was monitored by the phenol+sulfuric acid method, and the single peak part was combined, followed by concentration, dialysis and Ethanol precipitation to obtain an extract, which contains 80% β-1,3-D-glucan with a molecular weight of 200,000-500,000 Daltons. After natural drying, a white or off-white powder is obtained, which is recorded as: KA-233.

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Abstract

The invention discloses an extract containing beta-1,3-D-glucan, which is prepared by hydrolyzing sparassis crispa for 3 hours in present of beta-1,3-glucosidase under a condition of a temperature of 28 to 30 DEG C to hydrolyze part of the glucan in the sparassis crispa, wherein the molecular weight of the contained beta-1,3-D-glucan is 20,000 to 50,000 daltons. The prepared extract has remarkable tumor-inhibiting, cancer-preventing and anticancer functions, can regulate blood sugar, assistant in treatment of diabetes, lower blood fat and cholesterol and relieve cardiovascular and cerebrovascular disease, has anti-fatigue, aging-delaying, infection-resistance, anti-allergy, freckle-removing and whitening effects and can be prepared into various functional health-care food or medicines.

Description

technical field [0001] The present invention relates to an extract obtained by directional control enzymolysis technology, in particular to a β-1,3 glucosidase containing β- -Extract of 1,3-D-glucan, and its application in anti-tumor, immune regulation and auxiliary treatment of diabetes, cardiovascular and cerebrovascular diseases, etc. Background technique [0002] Hydrangea (Sparassis crispa (Wulf.) Fr.), also known as hydrangea mushroom, hydrangea mushroom, cauliflower mushroom, petal velvet, is a rare and precious edible fungus. Hydrangeas belong to the subclass Holobasidiomycetidae, Aphyllophorales, Sparassidaceae, and Sparassis crispa Fr. in classification. There are many varieties of hydrangea. From the current investigation, it is found that there are at least 7 genera of Hydrangeaceae. In my country, there are more than 10 species of wild hydrangeas. Due to different places of origin and different climatic conditions, the color, appearance and nutritional content ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/14C12P19/08A23L1/09A61K31/716A61P35/00A61P37/02A61P3/10A61P9/00C12R1/645
Inventor 吕正兵董淮海叶甫云
Owner SHANGHAI KEAI BIOLOGICAL TECH
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