A kind of preparation method of donkey-hide gelatin peptide

A technology of donkey-hide gelatin peptide and donkey-hide gelatin peptide, which is applied in the field of enzymatic hydrolysis of donkey-hide gelatin peptide, which can solve the problems of non-concentrated molecular weight distribution, high cost, salty taste and large molecular weight, and achieve the effect of concentrated molecular weight, low cost and small molecular weight

Inactive Publication Date: 2011-12-14
JIANGZHONG PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The object of the present invention is to provide a kind of extraction method of donkey-hide gelatin peptide, using donkey-hide gelatin as raw material and using alkaline protease to enzymolyze or neutral protease or trypsin for enzymatic hydrolysis, the enzymatic solution is concentrated and refined and spray-dried to obtain donkey-hide gelatin peptide products

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] 1. Weigh 20kg of donkey-hide donkey-hide gelatin that has been crushed and passed through a 80-mesh sieve, add 160kg of distilled water, stir evenly, and heat up to 85°C to inactivate lipase for 30 minutes;

[0028] 2. Cool to 60°C, add 400g of alkaline protease, stir for 4 hours, then heat up to 100°C, inactivate the enzyme for 30 minutes;

[0029] 3. Filtrate, the filtrate concentration density is 1.05-1.1, spray dry to get the finished donkey-hide gelatin peptide, and obtain 9.70kg powder.

[0030] Determination of donkey-hide gelatin oligopeptide content

[0031] 1. method summary

[0032] Low molecular weight protein hydrolyzate (including peptides and free amino acids) is soluble in trichloroacetic acid solution; high molecular weight protein is easy to precipitate in trichloroacetic acid solution. After the sample is dissolved in trichloroacetic acid solution, the precipitated protein substance is separated by centrifugation, and the acid-soluble protein conte...

Embodiment 2

[0072] 1. Weigh 20kg of donkey-hide donkey-hide gelatin that has been crushed and passed through a 80-mesh sieve, add 120kg of distilled water, stir evenly, and heat up to 85°C to inactivate lipase for 30 minutes;

[0073] 2. Cool to 70°C, add 10g of trypsin, stir for 4 hours, then heat up to 85°C, inactivate the enzyme for 15 minutes;

[0074] 3. Filtrate, the filtrate concentration density is 1.05-1.1, spray dry to get the finished donkey-hide gelatin peptide, and obtain 10.20kg powder.

Embodiment 3

[0076] 1. Weigh 20kg of donkey-hide donkey-hide gelatin that has been crushed and passed through a 100-mesh sieve, add 300kg of distilled water, stir evenly, and heat up to 120°C to inactivate lipase for 60 minutes;

[0077] 2. Cool to 40°C, add 100g of neutral protease, stir for 4 hours, then heat up to 110°C, inactivate the enzyme for 10 minutes;

[0078] 3. Filtrate, the filtrate is concentrated to a density of 1.05-1.1, and spray-dried to obtain the finished donkey-hide gelatin peptide, obtaining 11.30 kg of powder.

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PUM

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Abstract

The object of the present invention aims at the disadvantages of the prior art enzymatic hydrolyzate bitterness, salty taste, large molecular weight, non-concentrated molecular weight distribution, and high cost, and provides a method for extracting donkey-hide gelatin peptides. Or neutral protease or trypsin for enzymatic hydrolysis, the enzymatic solution is concentrated and refined technology and spray-dried to obtain the donkey-hide gelatin peptide product. The method has the advantages of simple operation, good taste, low cost, environmental protection and high efficiency, and is very suitable for large-scale industrial production.

Description

technical field [0001] The invention relates to the technical field of deep processing of donkey-hide gelatin, in particular to the preparation of donkey-hide gelatin peptides by enzymatic hydrolysis with biological enzymes [0002] Preparation. Background technique [0003] Ejiao mainly contains collagen (80%), and it is generally taken orally, while the protein in food or medicine is usually enzymatically hydrolyzed into oligopeptides or amino acids by the digestive juice to be absorbed and utilized by the body, and the above indications are common Weak spleen and stomach or gastrointestinal reactions, so the activity of digestive tract enzymes is low, and the digestion ability is poor. Therefore, oral administration of donkey-hide gelatin will not only cause a burden on the patient's digestive system, but also fail to achieve the desired drug effect. [0004] At present, the methods for extracting donkey-hide gelatin oligopeptides mainly include lye extraction and biolog...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06
Inventor 钟虹光易敏之卢建中刘根云马莉
Owner JIANGZHONG PHARMA
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