Dynamic liquid-liquid-solid imprinted microextraction-liquid chromatography on-line combined system and its application

Abstract

The invention discloses an on-line coupling system for a dynamic liquid-liquid solid imprinted microextraction-liquid phase chromatogram, which comprises a magnetic agitator, a water bath bottle, an extracting bottle, a draft tube, a peristaltic pump, a high-pressure six-way switching valve, a molecularly imprinted solid phase extracting material, a high-pressure six-way sample valve, a quantifying cup, a sample needle, a liquid phase chromatographic pump, a chromatographic column, a detector, a liquid phase chromatogram and the like. Through the coupling of a dynamic liquid-liquid solid imprinted microextracting device and the liquid phase chromatogram, the on-line overall processes of the preenrichment, the extraction, the desorption, the separation and the detection of a target analytein a water phase sample are achieved. By using the system, the universality of liquid-liquid extraction, the high selectivity and the high enrichment capability of a molecularly imprinted solid phasemicroextraction technique, the efficient separation capability and the high sensitivity of the liquid phase chromatogram are combined; the analysis degree of automation is improved; the analysis timeis shortened; the accuracy and the precision are improved; and the system can be effectively applied to the analysis and the detection of a trace organic matter in a complicated sample.

Description

Technical field [0001] The invention belongs to the field of analytical chemical sample pretreatment, and specifically relates to the design, analysis and application of a dynamic liquid-liquid-solid imprinting microextraction-liquid chromatography on-line combined system, which is suitable for trace traces in complex samples such as environment, food, medicine, biology, etc. Online pretreatment, extraction, desorption, separation and detection of large amounts of organic matter. Background technique [0002] Liquid-liquid-extraction (LLE) is a traditional general-purpose extraction technology, which is simple to use and has wide applicability. It is especially suitable for the extraction and enrichment of non-polar substances in aqueous samples. However, since the selectivity of liquid-liquid extraction is mainly determined by the partition coefficient of the analyte in the organic phase and the aqueous phase, it does not have high specificity and selectivity for extraction and ...

AI Technical Summary

Problems solved by technology

This method takes a long time, is cumbersome to operate, has large errors, and is unfavorable to be combined with subsequent analysis techniques to achieve online analysis.

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