A kind of silk support and its preparation and application

A technology of silk and silk fibroin, applied in medical science, prosthesis, coating, etc., can solve problems such as curling, uneven structure, and affecting the use effect of silk stents, and achieve smooth structure, simple preparation method, and good body phase. capacitive effect

Active Publication Date: 2011-12-28
ZHEJIANG XINGYUE BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Silk mesh stents treated with desericin protein are prone to curling, and the structure is relatively uneven; affecting the use effect of silk stents

Method used

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  • A kind of silk support and its preparation and application
  • A kind of silk support and its preparation and application
  • A kind of silk support and its preparation and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Weaving thick silk into a mesh structure support, the specification is 100mm×20mm×0.5mm, and the mesh size is 1.0mm 2 , then with 0.2% Na 2 CO 3 Solution 200mL, boiled for 30min, repeated 3 times, rinsed 3 times with 200mL medical purified water, and dried at room temperature (25°C) for 12h to obtain the basic support; Sprayed on the basic support with 4% silk fibroin aqueous solution, the coating amount was 0.5 mL / cm 2 , and aired at room temperature (25°C) for 24 hours. After inner and outer packaging, it is sterilized by cobalt-60 irradiation to obtain the silk scaffold.

Embodiment 2

[0038] Weave the thick silk into a mesh structure support, the specification is 100mm×100mm×0.5mm, and the mesh size is 1.0mm 2 , then with 0.2% Na 2 CO 3 Solution 200mL, boil for 30min, repeat 3 times. Rinse 3 times with 200mL medical purified water, dry in an oven at 40°C for 4h, and set aside. Spray-coat the pre-prepared 10% chitosan aqueous solution on the silk support, and the coating amount is 1.5mL / cm 2 , after the coating is completed, it is dried in a constant temperature oven at 40°C for 4 hours, and after inner and outer packaging, it is sterilized by cobalt-60 radiation to obtain a silk scaffold.

Embodiment 3

[0040] Weave the thick silk into a mesh structure support, the specification is 100mm×100mm×0.5mm, and the mesh size is 4.0mm 2 , then with 0.2% Na 2 CO 3 Solution 200mL, boil for 30min, repeat 3 times. Rinse 3 times with 200mL medical purified water, dry at room temperature (25°C) for 24h, and set aside. Spray-coat the pre-prepared 20% chitosan aqueous solution on the silk support, and the coating amount is 0.5mL / cm 2 After coating, it is dried in a constant temperature oven at 30°C for 12 hours, and after packaging inside and outside, it is sterilized by cobalt-60 radiation to obtain a silk scaffold.

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Abstract

The invention discloses a silk stent a preparation method and application to repairing and constructing of organism tissues. The silk stent is mainly composed of a base layer membrane and a water-soluble natural polymer material layer membrane, wherein the base layer membrane is a netting structure woven from silk, and the size of meshes is between 0.25mm<2> and 25mm<2>; and the water-soluble natural polymer material is silk fibroin, hyaluronic acid, collagen or chitosan. The invention has the advantages that: (1) the silk stent has a flat structure, excellent biological and mechanical performances and excellent tissue compatibility; (2) the meshes of the silk stent are uniform in size, and can be regulated; (3) the materials are easily available, and the preparation method is simple and convenient; (4) the natural polymer material adopted in the silk stent has good biological compatibility, and the operation is easy; and (5) the silk stent is suitable for repairing or reinforcing tissues inside an organism, and constructing tissues outside the organism.

Description

(1) Technical field [0001] The invention relates to a tissue engineering mesh support, in particular to a mesh silk support which can be used for tissue repair or reinforcement in vivo and tissue construction in vitro and a preparation method thereof. (2) Background technology [0002] Tissue defect and weakness are common problems in clinical practice. The causes include congenital defects, pathological factors, and aging. These soft tissue defects and weaknesses usually cannot be repaired by themselves, which greatly affects the function and appearance of the corresponding organs and tissues. Stress urinary incontinence, especially due to postpartum or postmenopausal pelvic relaxation, is a common condition among women. Since the mid-1990s, it has become one of the top 5 diseases in the world. At present, tissue patches are mainly used clinically to repair tissue defects and weaknesses. According to the chemical composition and biological characteristics of the material...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61F2/02A61F2/50A61L27/34A61L27/28
Inventor 邹晓晖谢淑君陈隆坤
Owner ZHEJIANG XINGYUE BIOTECH
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