A method for isolation and identification of sea cucumber pathogenic bacteria

An identification method, a technology of pathogenic bacteria, applied in the field of microorganisms, can solve the problems that restrict the sustainable and stable development of the industry, economic losses, etc., and achieve the effects of rapid separation and identification of bacteria, early diagnosis, and simple process
CN102296038AInactive Publication Date: 2011-12-28DALIAN UNIV OF TECH
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
DALIAN UNIV OF TECH
Publication Date
2011-12-28
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a method for separating and identifying pathogenic bacteria of sea cucumber, which is characterized in that the tissue of sea cucumber containing target pathogenic bacteria is sterilized with 75% alcohol for 60-90 seconds, and then washed with sterile sea water. Homogenize the lesion tissue, add sterile PBS buffer solution dropwise, use an inoculation loop to dip the bacteria-containing solution and streak it on the agar medium suitable for the growth of the pathogenic bacteria of Apostichopus rot skin syndrome, cultivate it at 24-28°C, and then pass it through a specific The corresponding target bacteria can be obtained by purifying and screening a single colony in TCBS culture medium. The pathogenicity of the isolated bacteria is verified through the animal experiment of A. japonicus inoculation and the morphological structure of the bacteria is observed by scanning electron microscope. The 16srDNA PCR technology is further used for identification. Isolated pathogenic bacteria. The invention has the advantages of simple operation and easy method, can quickly and effectively screen out the pathogenic bacteria of sea cucumber rotting skin syndrome, and simultaneously provides ideas for rapid screening of pathogenic bacteria for other aquatic animal diseases.
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Description

technical field

[0001] The invention relates to a method for isolating and identifying sea cucumber pathogenic bacteria, which belongs to the technical field of microorganisms. Background technique

[0002] Due to the rapid development and non-standard operation of sea cucumber cultivation, the problem of diseases has become increasingly prominent, and various obvious diseases and large-scale deaths have appeared. The most serious disease in sea cucumber farming. It has caused heavy economic losses to the vast number of japonicus cultivators, and has seriously restricted the sustainable and stable development of this industry. In order to ensure the healthy development of the sea cucumber aquaculture industry, active research on the epidemiology and etiology of "rotting skin syndrome" is an essential basic work for the comprehensive prevention and treatment of the disease.

[0003] "Rotten skin syndrome" is the most common and most serious disease in sea cucumber cultivati...

Claims

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