Device and method for detecting sputum smear tubercle bacillus quickly

A technology for tuberculosis and smear, which is applied in the field of biomedical detection and diagnosis, and can solve the problems of low detection efficiency, slow focusing speed, and small dynamic range of microscopic images.

Inactive Publication Date: 2012-01-04
DONGHUA UNIV
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Problems solved by technology

[0005] The technical problem to be solved by the present invention is to provide a device and method for quickly detecting Mycobacterium tuberculosis in sputum smears, to realize the dynamic intelligent identification and counting of the continuous field of view of the same sputum smear, to solve the problem of slow focusing speed and detection efficiency of traditional machine vision detection systems. Problems such as low and small dynamic range of microscopic images

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  • Device and method for detecting sputum smear tubercle bacillus quickly
  • Device and method for detecting sputum smear tubercle bacillus quickly
  • Device and method for detecting sputum smear tubercle bacillus quickly

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Embodiment 1

[0030] The present invention adopts following equipment during implementation:

[0031] 1. The CCD model is HV1303UM, the main parameters: CMOS CCD with a resolution of 1280*1024; the optical size is 1 / 1.8″; the highest 1.3 million pixels; the highest horizontal resolution is 1280; the digital-to-analog conversion accuracy is 10bit; 45dB High signal-to-noise ratio (AGC OFF); automatic gain control (AGC) that can be turned on / off, digital gain multiples are: *2, *1, *0.5, *0.25; for 550nm light source, its sensitivity is: 2.1V / Lux-s; auto-tracking (ATW) / manually set black and white electric balance correction method; can accept 24V AC and 12V DC power supply.

[0032] 2. Microscopic imaging system 4: It adopts the German standardized DIN standard, which can be directly connected with DIN eyepiece or C-mount CCD camera. The length of the monocular is suitable for DIN standard, and any DIN standard lens can be connected to this microscope imaging system, and the magnification i...

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Abstract

The invention relates to a device and a method for detecting sputum smear tubercle bacillus quickly. The device comprises a support, a charge coupled device (CCD) image sensor, a microscopic imaging system, a computer system, a three-dimensional accurate control console, an X-axis motor, a Y-axis motor, a Z-axis motor, wherein a high-accuracy grating ruler with a grating sensor is arranged in the Z-axis direction of the support additionally; a light-emitting diode (LED) light source plate of which the light intensity is controllable is positioned on the lower side of the microscopic imaging system and is used as an object stage; and the computer system comprises three software functional modules, namely a control module, an image target fusion module and a neural network classification and counting module. The method comprises a visual sense area interpretation algorithm and an optimum detection path scanning algorithm which are used for image detection and a method for fusing targets twice in image segmentation. By the device and the method, the detection accuracy and efficiency of machine vision can be improved, and the scientific reference is provided for the clinical diagnosis and prevention and treatment of tuberculosis.

Description

technical field [0001] The invention belongs to the technical field of biomedical detection and diagnosis, and in particular relates to a device and method for rapidly detecting tuberculosis in sputum smears. Background technique [0002] Tuberculosis is a chronic infectious disease caused by Mycobacterium tuberculosis infection. Tuberculosis, also known as tuberculosis and "white plague", is an ancient infectious disease. It is one of the most threatening infectious diseases to humans in the world today. Among the infectious diseases caused by single etiology, the disease with the highest mortality rate is the number one infectious killer in developing countries and has become a major public health and social problem. [0003] The rapid development of science and technology has brought many new methods to the experimental diagnosis of tuberculosis, but bacteriological examination will still be an important means of tuberculosis experiment and diagnosis because of its object...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/34C12M1/36C12Q1/06
Inventor 钟平涂新星周凌君叶韬王士乐
Owner DONGHUA UNIV
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