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Culture medium added with plant hormone, and application thereof in schizochytrium limacinum fermentation

A plant hormone and Schizochytrium technology, applied in the direction of microorganisms, microorganisms, fungi, etc., to reduce fermentation costs, promote industrial development, and improve fermentation efficiency

Inactive Publication Date: 2012-01-04
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are no reports on the effects of plant hormones on Schizochytrium

Method used

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  • Culture medium added with plant hormone, and application thereof in schizochytrium limacinum fermentation
  • Culture medium added with plant hormone, and application thereof in schizochytrium limacinum fermentation
  • Culture medium added with plant hormone, and application thereof in schizochytrium limacinum fermentation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1: The effect of 6-BA on the biomass and DHA content of Schizochytrium

[0034] The Schizochytrium culture medium uses glucose as the carbon source and the concentration is 60g / L; the yeast extract is the nitrogen source and the concentration is 20g / L; the proportion of seawater in the medium is 50%.

[0035] Then 6-BA was added to the Schizochytrium culture medium at concentrations of 0.5 mg / L, 1 mg / L, 3 mg / L, 6 mg / L, and 10 mg / .

[0036] The Schizochytrium was inserted into the medium at an inoculum of 10%.

[0037] The fermentation culture temperature is 23±1°C, the pH value is 6, the rotation speed is 200 rpm, and the shaking culture is 4 days.

[0038] Such as figure 1 As shown, the results show that the effect of 6-BA on the biomass and DHA content of Schizochytrium is not obvious when the concentration of 6-BA is 0.5mg / L. Under the conditions of 11mg / L and 3mg / L, 6-BA has The growth of Schizochytrium and DHA content play a role in promoting the growth of Schizochy...

Embodiment 2

[0039] Example 2: The influence of KT on the biomass and DHA content of Schizochytrium

[0040] The composition and culture method of Schizochytrium culture medium are as described in Example 1.

[0041] Such as figure 2 As shown, the results show that KT can increase the biomass and DHA content of Schizochytrium at a low concentration (≤10mg / L). When KT is added at 10mg / L, the biomass of Schizochytrium reaches 9.55g / L. L·d, 2.25g / L·d higher than the control group; DHA content 14.9%, 1.9% higher than the control group. When the KT dosage is 15mg / L or higher (20mg / L), it has a significant inhibitory effect on the growth of Schizochytrium and the accumulation of DHA.

Embodiment 3

[0042] Example 3: Effect of GA on the biomass and DHA content of Schizochytrium

[0043] The composition and culture method of Schizochytrium culture medium are as described in Example 1.

[0044] GA was added to the medium at 0.5 mg / L, 1.0 mg / L, 3 mg / L, 6 mg / L and 10 mg / L.

[0045] Such as image 3 As shown, the results show that when the GA addition amount is 1 mg / L, the Schizochytrium biomass and DHA content both reach the maximum, which are 8.62 g / L·d and 17.2%, respectively, which are 1.32 g / L higher than the control group. L·d and 4.2%.

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Abstract

The invention provides a culture medium added with a plant hormone, and an application thereof in schizochytrium limacinum fermentation. The culture medium comprises components of: a carbon source of glucose with a concentration of 10 to 100g / L; a nitrogen source of an yeast extract with a concentration of 10 to 80g / L; seawater which takes 20% to 80% of the total volume of the culture medium; and one component selected from 6-benzyladenine, furfuryl aminopurine, gibberellin, indole butyric acid, naphthalene acetic acid, and heteroauxin. According to the invention, a plant hormone with an appropriate concentration is added to the schizochytrium limacinum fermentation medium. The cytokinins of 6-benzyladenine, furfuryl aminopurine, gibberellin, indole butyric acid, naphthalene acetic acid, and heteroauxin all assist in substantially improving the growing speed and the DHA content of schizochytrium limacinum. Also, with the technical scheme provided by the invention, fermentation cost of schizochytrium limacinum can be substantially reduced, and fermentation efficiency can be improved, such that the development of the industry is promoted.

Description

Technical field [0001] The invention belongs to the technical field of microbial application, and specifically relates to a medium added with plant hormones and its application in the fermentation of Schizochytrium. Background technique [0002] Studies have shown that n-3 polyunsaturated fatty acids (PUFAs) represented by Docosahexaenoic acid (DHA, C22:6, n-3) are essential fatty acids because they are beneficial The various physiological functions of human health have received extensive attention. DHA is a key component of the cerebral cortex and retina. It is essential for the normal development of the visual and nervous system of infants and young children, and to maintain the function of the visual and nervous system. In addition, it also has the physiological functions of preventing cardiovascular diseases, anti-cancer, and anti-inflammatory. [0003] Schizochytrium limacinum is a marine fungus. The most notable feature of this fungus is the accumulation of large amounts of...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12P7/64C12R1/645
Inventor 张学成臧晓南杨青徐涤许永
Owner OCEAN UNIV OF CHINA
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