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Endophytic fungi chaetomium globosum strain, microbial agent and application thereof

A technology of microbial agent and plant endophytic bacteria, which is applied in the direction of plant growth regulators, plant growth regulators, microorganisms, etc., can solve the problems of affecting the field biocontrol effect, unstable disease prevention effect, easy loss of vitality, etc., to achieve Effects of promoting growth and development, long storage time, and improving adaptability

Inactive Publication Date: 2014-05-07
SHANDONG AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although the application in production has achieved certain effects, the field application often leads to unstable disease control effects due to the influence of various environmental factors
The main reason is that the spore powder preparation of Trichoderma is its asexual conidia, which is easy to lose vitality and has a short shelf life, which seriously affects the biocontrol effect in the field.

Method used

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  • Endophytic fungi chaetomium globosum strain, microbial agent and application thereof
  • Endophytic fungi chaetomium globosum strain, microbial agent and application thereof
  • Endophytic fungi chaetomium globosum strain, microbial agent and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0116] Embodiment one: the field experiment that Chaetomium globosa ND35 bacterial strain promotes the growth of walnut, Chinese chestnut seedling

[0117] The experiment was carried out in the nursery of Guanghua Temple Forest District, Culaishan Forest Farm, Tai'an City from 2007 to 2010. First spread uniform organic fertilizers on the surface of the field, plow the ground deeply, and after leveling, make a ridge to make a furrow, the furrow width is about 1m, adjust the ditch (depth 15cm) to water, select good walnut seeds for germination and place them in the ditch, The row spacing of plants is 20cm×30cm, and they are arranged completely randomly so that the germination status in each treatment is roughly the same. The experiment was divided into two treatments. Process 1., the solid bacterial agent of the walnut seed of germination inoculation culture chaetelum ND35 strain, about 5g of every seed. Treatment ②, with blank as control. Appropriate concentration of phoxim ...

Embodiment 2

[0129] Embodiment two, the induced disease resistance effect of Chaetomium globosa ND35 strain to poplar cutting seedlings

[0130] The experiment was carried out at the Experimental Station of Plant Protection College in the South Campus of Shandong Agricultural University from June to October 2008.

[0131] (1) Field experiment to investigate the induced antagonistic effect of Chaetomium globosa ND35 strain on poplar leaf rust

[0132] Due to factors of environment and poplar species, poplar cutting seedlings were infected with leaf rust in June. In August, at 1.3m, 1.7m and 2.1m of the poplar, the incidence of the front (teliospore heap) and leaf back (ideliospore heap) of the naturally susceptible poplar leaves in the inoculation and control treatment groups were investigated. On diseased poplars, the grading standard is determined according to the ratio of the lesion area on the front and back of leaves at the same height to the total leaf area: no disease is grade 0, 0-...

Embodiment 3

[0145]Embodiment three, chaetelum ND35 bacterial strain is to the influence of ginger growth

[0146] The experiment was carried out at the experimental station of the College of Horticultural Science and Engineering, Shandong Agricultural University South Campus. Ginger was sown on May 5, 2010 after germination, with a row spacing of 65 cm and a plant spacing of 20 cm. The experiment was divided into two treatments: ①: Birth delivery and antibacterial control, ②: Blank control. Other field management is carried out as usual [7] . When harvested on October 19, five trees were randomly selected in groups, and the stem diameter, plant height, branch number, leaf number, leaf fresh weight, stem fresh weight, and underground fresh weight were measured respectively.

[0147] Growth rate=(average value of single plant in treatment group-average value of single plant in control group) / average value of single plant in control group×100%

[0148] The test results showed that the pl...

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Abstract

The invention discloses an endophytic fungi chaetomium globosum strain, a microbial agent and an application thereof. The strain is named as chaetomium globosum strain ND 35, the preservation organization is the China General Microbiological Culture Collection Center, the preservation date is 9th,10,2010, the preservation number is CGMCC No. 4136. The strain is a biological beneficial bacteria with a plurality of biological control effect mechanisms, and has the plant pathogen inhibition capability with broad-spectrum and high-efficiency, and also has capability for promoting a plurality of plants growth, and is suitable for industrial production, the shelf life is long which is suitable for preventing and treating the plant diseases, so that the strain is used for producing the biological bactericide products for preventing and treating a plurality of plant diseases and multifunctional biofertilizer products for promoting the growth of plants.

Description

technical field [0001] The invention belongs to the field of agricultural microorganisms, and the involved chaetomium globosum ND35 strain is a plant endophytic fungal strain with multi-mechanism and broad-spectrum ability to inhibit plant pathogenic bacteria and promote plant growth, and can be used for research and development of various Biological fungicide products for plant diseases and multifunctional biological fertilizer products for promoting plant growth. Background technique [0002] The use of live microbial agents to control plant diseases is one of the traditional biocontrol technologies. At present, the commercialized biocontrol fungi are mainly Trichoderma and the like. Although the application in production has achieved certain effects, the field application often results in unstable disease control effects due to the influence of various environmental factors. The main reason is that the spore powder preparation of Trichoderma is its asexual conidia, which...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A01N63/04A01P3/00A01P21/00C12N3/00C12R1/645
Inventor 高克祥刘晓光米士伟毕建杰张修国
Owner SHANDONG AGRICULTURAL UNIVERSITY
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