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Immunity reinforcing agent for Litopenaeus vannamei Boone

The technology of an immune enhancer, Vannabine, applied in the field of carboxymethyl zymosan, can solve the problems that the immune enhancer has no expected effect, the effect is not good, and the immunity and disease resistance are not significantly improved, so as to improve immunity and disease resistance, good effect, growth-promoting effect

Inactive Publication Date: 2012-01-18
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the effect of immune enhancer on Litopenaeus vannamei is not good, mainly because when the disease breaks out, the use of immune enhancer often has no expected effect
Zymosan is a commonly used immune enhancer in aquaculture, but it also has the disadvantages of not significantly improving the immunity and disease resistance of cultured prawns.

Method used

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  • Immunity reinforcing agent for Litopenaeus vannamei Boone
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  • Immunity reinforcing agent for Litopenaeus vannamei Boone

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Example 1: Production of carboxymethyl zymosan with different degrees of substitution

[0014] Take 6.2ml of 30% sodium hydroxide solution and 62.5ml of isopropanol, and mix the two at 10°C to prepare a reaction system. Slowly add 5 g of zymosan into the reaction system, react at 10° C. for 1 hour, and keep stirring during the reaction to fully proceed the reaction. Another 2.2 g of sodium chloroacetate was dissolved in 7 ml of deionized water, and the 7 ml of sodium chloroacetate aqueous solution was quickly added to the above reaction system. The reaction system was reacted at 70°C for 2 hours, during this period, the stirring was continued to fully proceed the reaction. After 2 hours, the pH of the reaction system was adjusted to 7.0 with 6 mol / L hydrochloric acid solution; finally, the reaction system was dialyzed with deionized water for 72 hours to obtain a solution of carboxymethylzymosan without chloride ions. The solution was vacuum dried to obtain carboxymet...

Embodiment 2

[0016] Embodiment 2: the determination of carboxymethyl zymosan substitution degree

[0017] Dissolve 1 g of carboxymethyl zymosan in 15 ml of acetone and mix thoroughly. Add 3ml6mol / L hydrochloric acid solution to the above system, react for 30 minutes, during this period, keep stirring to make it fully react. The reaction solution was filtered, and the filter residue was washed with 80% methanol to make the filter residue neutral. The filter residue was baked at 50°C for 2 hours to fully dry it. Take 0.5g of the filter residue, dissolve it in 20ml of 0.2mol / L sodium hydroxide solution, and dilute to 100ml with distilled water. Take 25ml constant volume solution, add 1 drop of phenolphthalein reagent, titrate with 0.05M hydrochloric acid until colorless, record the volume V1 used. Take another 20ml of 0.2mol / L sodium hydroxide solution, and dilute to 100ml with distilled water. Take 25ml constant volume solution, add 1 drop of phenolphthalein reagent, titrate with 0.05mol...

Embodiment 3

[0020] Embodiment 3: Determination of dissolution rate of zymosan and carboxymethyl zymosan

[0021] Take 2g of zymosan or carboxymethyl zymosan, add it to 10ml of deionized water, and shake for 20 minutes. After 20 minutes, the above mixture was centrifuged for 20 minutes, the supernatant was taken, and dried at 100°C. Weigh the dried powder and count M.

[0022] Dissolution rate = M / 2 × 100%

[0023] After measurement and calculation, the dissolution rate of carboxymethylzymosan A was 33.76%. The dissolution rate of carboxymethylzymosan B was 41.06%. The dissolution rate of carboxymethylzymosan C was 62.69%. The dissolution rate of carboxymethylzymosan D was 86.56%. The dissolution rate of zymosan was 4.07%.

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Abstract

The invention relates to an immunity reinforcing agent for Litopenaeus vannamei Boone. The immunity reinforcing agent is a carboxymethyl zymosan, the carboxymethyl substitution degree of which is 0.348 to 0.356. The immunity reinforcing agent is prepared by adding sodium chloroacetate to react with zymosan in an alkali reaction system. Meanwhile, the invention also relates to bait used with the carboxymethyl zymosan immunity reinforcing agent. When the immunity reinforcing agent is used in the prawn feed, the addition quantity is only 0.1 to 0.2 percent, the growth of the Litopenaeus vannameiBoone can be obviously promoted, and the immunity and the disease resistance of the Litopenaeus vannamei Boone are remarkably improved. The application effect of the immunity reinforcing agent is obviously better than that of the zymosan. Meanwhile, when the immunity reinforcing agent is used with the Litopenaeus vannamei Boone bait prepared by the applicant, better effect can be obtained.

Description

technical field [0001] The invention belongs to the technical field of immunity enhancers for aquatic feed, and in particular relates to an immunity enhancer for Litopenaeus vannamei, that is, carboxymethyl zymosan that can improve the immunity and disease resistance of Litopenaeus vannamei. Background technique [0002] The disease of cultured prawns is a worldwide problem, which is caused by the degradation of germplasm and the deterioration of the culture environment due to the one-sided pursuit of yield. Between 1996 and 2010, worldwide losses due to shrimp diseases amounted to US$ 2.75 billion. Immunopotentiators refer to a class of substances that can promote or induce host defense responses and enhance the ability of biological organisms to resist diseases. disease resistance. Compared with antibiotics and vaccines, immune enhancers have the advantages of safety, no residue, wide range of action and convenient use. However, the effect of immune enhancer on Litopena...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/18A23K1/16A23K10/22A23K10/30A23K20/158A23K20/163A23K20/174A23K20/20A23K20/22A23K50/80
CPCY02A40/818
Inventor 张文兵白楠徐玮谷珉麦康森
Owner OCEAN UNIV OF CHINA
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