Preparation method and application of fluorescence mesoporous silica nano-particle

A technology of mesoporous silica and nanoparticles, applied in the field of fluorescent nanomaterials, can solve problems such as limited application, weak fluorescent emission, and difficulty in uniform and stable dispersion

Inactive Publication Date: 2012-01-18
NORTHEAST NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of the currently prepared fluorescent mesoporous nanomaterials are not easy to uniformly and stably disperse in water, and exhibit weak fluorescence emission, which limits their application in the field of biology.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Preparation of mercapto-functionalized mesoporous silica nanoparticles (MSN-SH 1 ):

[0029] Mix 28.6g of triethanolamine (TEA), 4.46mL (20mmol) of tetraethyl orthosilicate (TEOS) and 0.38mL (2mmol) of mercaptopropyltriethoxysilane (KH-590), and heat to 90°C in an oil bath °C, after 20 min, mixed with 53.4 g of CTACl aqueous solution (CTACl, 2.5 wt%) heated to 60 °C, the stirring speed was 600 rpm, the temperature was maintained at 23 °C, and the stirring was continued for 4 hours. After the reaction, 100 mL of ethanol was added for precipitation, and the obtained sample was centrifuged at 9500 rpm for 15 min. The sample obtained by centrifugation was extracted three times with template extract solution (volume ratio of ethanol to hydrochloric acid: 8:1) in an ice-water bath, ultrasonically extracted for 30 min each time, centrifuged and washed twice with water and once with ethanol, and vacuum-dried at room temperature. The resulting mesoporous silica nanoparticles (...

Embodiment 2

[0031] Preparation of mercapto-functionalized mesoporous silica nanoparticles (MSN-SH 2 ):

[0032] 2.23g (10mmol) TEOS and 0.38mL (2mmol) mercaptopropyltriethoxysilane (KH-590) were used to replace the feed ratio in Example 1, and CTAB was used as the template. Other experimental conditions and methods are as described in Example 1. The obtained mesoporous silica nanoparticles (MSN-SH 2 ) has a BET specific surface area of ​​765m 2 g -1 , the pore volume is 0.43cm 3 g -1 .

Embodiment 3

[0034]8-hydroxyquinoline zinc functionalized mesoporous silica nanoparticles (MSN-SH 1 -Znq):

[0035] Mercapto-functionalized mesoporous silica nanoparticles (MSN-SH 1 )1.0g dispersed in 100mL 0.05mol L -1 In an aqueous solution of zinc acetate, stir at room temperature for 12 hours, centrifuge and wash to obtain mesoporous silica nanoparticles (Zn-MSN-SH) adsorbed with zinc ions. 1 ). 0.7g of Zn-MSN-SH 1 Disperse in distilled water, then add 100mL 4.7×10 -3 mol L -1 The ethanol solution of octahydroxyquinoline was then stirred at room temperature for 48 hours, washed by centrifugation, and vacuum-dried at room temperature. BET specific surface area 1078m 2 g -1 , pore volume: 0.64cm 3 g -1 ; Fluorescence emission at 506 nm under excitation at 350 nm. It has excellent fluorescence stability, and the fluorescence intensity in the aqueous solution of pH=3 and pH=10 can remain unchanged for more than 3 months.

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Abstract

The invention relates to a preparation method of two fluorescence mesoporous silica nano-particles, and belongs to the field of fluorescence nano-materials. According to the invention, an in-situ formation method is firstly adopted; 8-hydroxyquinoline is directly coordinated with zinc ions complexed in mesoporous silica, and conjugated polymer polyphenyleneethynylene precursor disulphonium salts are directly introduced into mesoporous silica nano-particles through an ion exchange process; and thus a fluorescence mesoporous silica nano-material is obtained with stable chemical and thermodynamic properties, which is a mesoporous silica nano-particle functionalized by 8-hydroxyquinoline and fluorescence conjugated polymer. Based on the high specific surface area, stable fluorescence performance, good dispersibility and biocompatibility of the fluorescence mesoporous nano-material, the prepared fluorescence mesoporous nano-material is applicable to biological aspects such as drug slow release, cell marking, gene vector, and the like.

Description

technical field [0001] The invention relates to a preparation method and a biological application of two mesoporous silicon dioxide fluorescent nanoparticles, belonging to the technical field of fluorescent nanometer materials. Background technique [0002] Mesoporous materials have uniform and adjustable mesoporous (2-50nm) channels, stable framework structure, easy-to-modify inner surface, amorphous framework with certain wall thickness and easy doping, and high specific surface area. Mesoporous SiO 2 The advantages of high thermal stability, stable mechanical properties, no biological activity and no toxicity make it have great potential in biological applications. In particular, mesoporous silica nanoparticles can be used as drug and gene carriers because they can be internalized by cells, and their small size (generally less than 300nm) allows internalization through the cell membrane, and then local release of the adsorbed drugs. At present, people's research on meso...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C01B33/12C09K11/06B82Y40/00B82Y30/00A61K47/32C12N15/63G01N21/64
Inventor 吕长利曲颖付玉芹何瑶
Owner NORTHEAST NORMAL UNIVERSITY
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