Method for extracting total saponins from fructus gleditsiae
A technology of pig tooth soap and total saponins, which is applied in the field of extracting pig tooth soap total saponins by column chromatography and membrane separation technology, can solve the problems of large sample loss and unfavorable environmental protection, and achieve less sample loss, easy to master, and simple method Effect
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Embodiment 1
[0021] Grind the dried medicinal material of pig tooth soap, take 1kg, add 8 times the amount of 60% ethanol aqueous solution, ultrasonically extract twice, the extraction frequency is 30KHz, filter to obtain the extract, concentrate under reduced pressure until there is no alcohol smell, add HPD300 macroporous resin column, The amount of macroporous resin is 1kg, first wash away impurities with water, then elute with 55% ethanol solution, collect the eluate, recover ethanol under reduced pressure, filter through a hollow ultrafiltration membrane with a molecular weight cut-off of 5000-10000, and then filter the filtrate Concentrate through a hollow nanofiltration membrane with a molecular weight cut-off of 1000, and freeze-dry to obtain 142 g of total saponins of saponins.
[0022]
Embodiment 2
[0024] Grind the dried medicinal materials of pig tooth soap, take 1kg, add 6 times the amount of 75% ethanol aqueous solution, ultrasonically extract twice, the extraction frequency is 25KHz, filter to obtain the extract, concentrate under reduced pressure until there is no alcohol smell, add XAD-16 macroporous resin Column, the amount of macroporous resin is 500g, first use 20% ethanol solution to wash away impurities, then use 60% ethanol solution to elute, collect eluent, recover ethanol under reduced pressure, and pass through a hollow ultra-thin filter with a molecular weight cut-off of 5000-10000 Filtrate through a filter membrane, and then concentrate the filtrate through a hollow nanofiltration membrane with a molecular weight cut-off of 1000, and freeze-dry to obtain 125 g of total saponins of saponins.
[0025]
Embodiment 3
[0027] Grind the dried medicinal material of pig tooth soap, take 3kg, add 5 times the amount of 45% ethanol aqueous solution, ultrasonically extract 3 times, the extraction frequency is 50KHz, filter to obtain the extract, concentrate under reduced pressure until there is no alcohol smell, add HP20 macroporous resin column, The amount of macroporous resin is 1.5kg. First, wash away impurities with water, then elute with 55% ethanol solution, collect the eluate, recover ethanol under reduced pressure, filter through a hollow ultrafiltration membrane with a molecular weight cut-off of 5000-10000, and the filtrate Concentrate through a hollow nanofiltration membrane with a molecular weight cut-off of 1000, and freeze-dry to obtain 440 g of total saponins of the saponins.
[0028]
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