Method for extracting total saponins from fructus gleditsiae

A technology of pig tooth soap and total saponins, which is applied in the field of extracting pig tooth soap total saponins by column chromatography and membrane separation technology, can solve the problems of large sample loss and unfavorable environmental protection, and achieve less sample loss, easy to master, and simple method Effect

Inactive Publication Date: 2012-01-25
NANJING ZELANG AGRI DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above method uses organic solvent extraction, which is not conducive to environmental protection, and at the same time, the sample loss is large

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Grind the dried medicinal material of pig tooth soap, take 1kg, add 8 times the amount of 60% ethanol aqueous solution, ultrasonically extract twice, the extraction frequency is 30KHz, filter to obtain the extract, concentrate under reduced pressure until there is no alcohol smell, add HPD300 macroporous resin column, The amount of macroporous resin is 1kg, first wash away impurities with water, then elute with 55% ethanol solution, collect the eluate, recover ethanol under reduced pressure, filter through a hollow ultrafiltration membrane with a molecular weight cut-off of 5000-10000, and then filter the filtrate Concentrate through a hollow nanofiltration membrane with a molecular weight cut-off of 1000, and freeze-dry to obtain 142 g of total saponins of saponins.

[0022]

Embodiment 2

[0024] Grind the dried medicinal materials of pig tooth soap, take 1kg, add 6 times the amount of 75% ethanol aqueous solution, ultrasonically extract twice, the extraction frequency is 25KHz, filter to obtain the extract, concentrate under reduced pressure until there is no alcohol smell, add XAD-16 macroporous resin Column, the amount of macroporous resin is 500g, first use 20% ethanol solution to wash away impurities, then use 60% ethanol solution to elute, collect eluent, recover ethanol under reduced pressure, and pass through a hollow ultra-thin filter with a molecular weight cut-off of 5000-10000 Filtrate through a filter membrane, and then concentrate the filtrate through a hollow nanofiltration membrane with a molecular weight cut-off of 1000, and freeze-dry to obtain 125 g of total saponins of saponins.

[0025]

Embodiment 3

[0027] Grind the dried medicinal material of pig tooth soap, take 3kg, add 5 times the amount of 45% ethanol aqueous solution, ultrasonically extract 3 times, the extraction frequency is 50KHz, filter to obtain the extract, concentrate under reduced pressure until there is no alcohol smell, add HP20 macroporous resin column, The amount of macroporous resin is 1.5kg. First, wash away impurities with water, then elute with 55% ethanol solution, collect the eluate, recover ethanol under reduced pressure, filter through a hollow ultrafiltration membrane with a molecular weight cut-off of 5000-10000, and the filtrate Concentrate through a hollow nanofiltration membrane with a molecular weight cut-off of 1000, and freeze-dry to obtain 440 g of total saponins of the saponins.

[0028]

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Abstract

The invention relates to a method for extracting total saponins from fructus gleditsiae, which comprises the following steps of: firstly, crushing fructus gleditsiae dried medicinal material, adding 5-8 volumes by weight of lower alcohol water solution, carrying out ultrasound extraction for 2-3 times and filtering to obtain an extraction solution; secondly, carrying out condensation under reduced pressure on the extraction solution until no alcohol flavor exists, adding the extraction solution in a macroporous resin column, washing with water or 10-20 percent ethanol solution to remove impurities, eluting with 55-70 percent of ethanol solution and collecting an eluent; and thirdly, reducing the pressure and recovering the ethanol from the eluent, filtering through an ultrafiltration membrane, condensing filtrate through a nanofiltration membrane and drying to obtain the total saponins from the fructus gleditsiae. The method has the advantages of less pollution, low cost and high yield; and the obtained product has no solvent residue and can be directly used for producing foods and medicines.

Description

technical field [0001] The invention relates to a method for extracting total saponins from pig tooth soap, in particular to a method for extracting total saponins from pig tooth soap by column chromatography and membrane separation technology. Background technique [0002] Pig tooth soap is the dry sterile fruit of the plant Acacia saponaria in the subfamily of Fabaceae. Honey locust is an endemic species distributed in my country, and its distribution and cultivation coverage area accounts for about 50% of the country's land area. Acacia saponins can form two kinds of fruits under different development conditions. The mature and fat ones are big saponins, and the underdeveloped and thin ones are pig tooth soaps. Pig tooth soap was included in the 2005 edition of "Chinese Pharmacopoeia" as one of the commonly used traditional Chinese medicines. Pig tooth soap has the effects of eliminating phlegm, resuscitation, swelling and stagnation, and is mainly used for apoplexy, un...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/483A61P9/10A61P29/00A61P31/12A61P35/00A61P37/08
Inventor 苏刘花
Owner NANJING ZELANG AGRI DEV
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