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Sealing and stabilizing agent for microporous board

A technology for sealing stabilizers and microporous plates, which can be used in measuring devices, instruments, scientific instruments, etc., and can solve the problem of high prices

Active Publication Date: 2012-01-25
GUANGZHOU YOUDI BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, whether it is one-step or two-step, the currently used stabilizer formulations are highly commercially confidential, and most of them are expensive

Method used

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  • Sealing and stabilizing agent for microporous board
  • Sealing and stabilizing agent for microporous board

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Tris 45mmol

[0024] BSA 0.2g

[0025] PVP-40 0.5g

[0026] Sucrose 8g

[0027] Tween-20 0.02ml

[0028] Sodium azide 0.05g

[0029] The above components were dissolved in 90ml of water, the pH value was adjusted to 7.2 with concentrated HCl, and the volume was adjusted to 100ml.

[0030] Add the above-mentioned blocking stabilizer and 0.5% BSA solution (this is used as a control) to the microwell plate coated with anti-carcinoembryonic antigen (CEA) antibody, add 280 microliters to each well, and then incubate at room temperature for 2 hours. Pour out all the liquid in the microwell plate and pat the plate dry, place it in a dry box to dry and then vacuum pack it. The microplates were placed at 37°C and 60°C for 7 days, and the microplates at day 0 were used as controls, and the residual activity of the microplates after being placed at 37°C and 60°C for 7 days was detected and calculated by time-resolved immunofluorescence.

[0031] Table 1 Residual activity of ...

Embodiment 2

[0034] Sodium dihydrogen phosphate 50mmol

[0035] BSA 0.3g

[0036] PVP-40 0.5g

[0037] Trehalose 7.5g

[0038] Tween-20 0.01ml

[0039] ProClin 300 0.05g

[0040] Dissolve the above components in 90ml of water, adjust the pH value to 7.2 with concentrated NaOH solution, and set the volume to 100ml.

[0041] Add the above-mentioned blocking plate stabilizer and 0.5% BSA solution (this is used as a control) to the microwell plate coated with hepatitis B surface antigen (HBsAg), add 280 microliters to each well, and then incubate at room temperature for 2 hours. All the liquid in the orifice plate was poured out and the plate was patted dry, placed in a drying box to dry and then vacuum packed. The microplates were placed at 37°C and 60°C for 7 days, and the microplates at day 0 were used as controls, and the residual activity of the microplates after being placed at 37°C and 60°C for 7 days was detected and calculated by time-resolved immunofluorescence.

[0042]

[00...

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Abstract

The invention discloses a sealing and stabilizing agent for a microporous board. In terms of 100ml water, 0.1-3g of protein, 3-20g of saccharide, 0.01-1g of organic polymer, 0.01-0.05ml of nonionic surfactant, 0.01-0.1g of preservative and 10-100mmol of buffer solution are contained. The sealing and stabilizing agent disclosed by the invention is researched and designed on account of the characteristic that antigen or antibodies coated on a stationary phase are easy to inactivate. The sealing and stabilizing agent includes not only sealing components but also organic components and inorganic components, wherein the sealing components can be used for effectively sealing excessive loci on a sealing board, and the organic components and the inorganic component can be used for effectively stabilizing the antigen or the antibodies, so that the sealing purpose and the stabilizing purpose can be organically combined together through one-step operation. The sealing and stabilizing agent disclosed by the invention can be applied to immunological detection methods such as enzymelinked immunosorbent assay, chemiluminesent immunoassay, time resolved fluoroimmunoassay and the like, wherein the microporous board serves as the stationary phase; and the sealing and stabilizing agent can be used for effectively stabilizing the activity of the antigen or the antibodies coated on a stabilizing board.

Description

technical field [0001] The present invention relates to a closed plate stabilizer for microwell plates. [0002] Background technique [0003] Immunoassay technology has been widely used in the field of biomedicine today, and has become a necessary conventional technical means in clinical, scientific research, environmental and health testing, forensic identification and many other departments, playing an irreplaceable role. Related detection products also have a huge market. Immunoassay techniques include enzyme-linked immunoassay, chemiluminescence immunoassay, time-resolved immunofluorescence analysis, etc. Most of the stationary phases used in these detection techniques are microwell plates. After the antigen or antibody is coated on the stationary phase of the microwell plate, the structure of the antigen or antibody may change or even partially denature due to passive adsorption or coupling, transportation and long-term storage, resulting in the activity of the antig...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/543
Inventor 王征李来庆史志东刘彩娥王燕张立成
Owner GUANGZHOU YOUDI BIOTECH CO LTD
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