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Application of flavonoids compounds to breast cancer resistance

A compound and breast cancer technology, applied in medical preparations containing active ingredients, antineoplastic drugs, drug combinations, etc., can solve the problems of high toxicity and side effects

Active Publication Date: 2012-02-01
PEKING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Existing anticancer drugs (chemical drugs) have high toxicity and side effects, and cannot solve the problems of drug resistance, recurrence and metastasis of tumor stem cells; therefore, finding low-toxic and effective new breast cancer treatment drugs is a research and development hotspot in recent years

Method used

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  • Application of flavonoids compounds to breast cancer resistance
  • Application of flavonoids compounds to breast cancer resistance
  • Application of flavonoids compounds to breast cancer resistance

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Dried lobular lotus medicinal material, about 18.0kg after crushing, reflux extraction twice with 8 times the amount of 95% ethanol, the extraction time is 2 hours and 1 hour respectively, the ethanol is recovered under reduced pressure, and the extract with a relative density of 1.10 when concentrated to 70°C About 4.2kg. Take about 4.0kg, add 12L of distilled water and stir until completely suspended, then extract 7 times with equal volume of ethyl acetate and 6 times with water-saturated n-butanol. The solvent was recovered from the extract under reduced pressure to obtain various extraction fractions. Take 1100 g of ethyl acetate, and separate it by silica gel column (200-300 mesh) chromatography, petroleum ether-acetone (20:1-0:20), methanol gradient elution, collect one fraction per 1000 ml, and collect 576 fractions in total. split. According to the TLC test results (Qingdao ocean silica gel self-laying plate; chromogen: 1% vanillin-concentrated sulfuric acid; ...

Embodiment 2

[0024] Determination of lemon phenol (1), 6-prenyl quercetin 3-methyl ether (2), 1,3,7,8-tetrahydroxyl by acid phosphatase method Inhibitory effects of ketone (3), 6-prenyl kaempferol 3-methyl ether (4), and paclitaxel (provided by the Third Hospital of Peking University) on human breast cancer cells.

[0025]Human breast cancer MDA-MB-231 cell line (provided by Tumor Biology Center, Peking University Health Science Center) and human breast cancer T47D cell line (provided by Peking University Health Science Center, Experimental Animal Center) were both treated with a volume fraction of 10% newborn bovine serum (USA Gibco company), 100U / mL penicillin, 100U / mL streptomycin in DMEM medium (U.S. Gibco company) culture, 37 ℃, 95% humidity, 5% CO 2 Incubator conditions (Napco, USA); routine 2.5g / L trypsin digestion and passage.

[0026] After digesting the cells in the logarithmic growth phase, make a cell suspension by 1.5×10 3 The density per well was inoculated in a 96-well cu...

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Abstract

The invention discloses application of four kinds of known flavonoids compounds to treatment of breast cancers. The four kinds of known flavonoids are known compoundes obtained by separating fruit of sinopodophyllum emodi and have structures shown as structural formulas 1-4 in the specification. Experiments on the effect of the kinds of compounds on inhibiting human breast cancer cells show that the series of compounds have a favorable effect on inhibiting the proliferation of the breast cancer cells.

Description

technical field [0001] The invention relates to the application of flavonoids in treating breast cancer. Background technique [0002] Breast cancer is one of the malignant tumors with the highest incidence rate in women, which seriously threatens women's health and life. According to statistics, about 1.2 million women in the world suffer from breast cancer every year, and 500,000 patients die from the disease. So far, there is no satisfactory treatment for breast cancer except surgery. Existing anticancer drugs (chemical drugs) have high toxicity and side effects, and cannot solve the problems of drug resistance, recurrence and metastasis of tumor stem cells; therefore, finding low-toxic and effective new breast cancer treatment drugs is a research and development hotspot in recent years. [0003] Sinopodophylli Fructus is a commonly used Tibetan medicine, and its source is recorded in the 2010 edition of the Chinese Pharmacopoeia as the dried ripe fruit of Sinopodophyll...

Claims

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Application Information

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IPC IPC(8): A61K31/352A61P35/00
Inventor 尚明英王庆辉肖军军尚应辉孔越蔡少青
Owner PEKING UNIV
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