Preparation method of ractopamine bionic antibody

Abstract

The invention relates to a preparation method of a ractopamine bionic antibody. The preparation method is mainly and technically characterized in that the ractopamine bionic antibody having high selective recognition ability for ractopamine is synthesized by adopting a molecular imprinting technology and an organic-inorganic hybrid technology, with the ractopamine as a template molecule, methacrylic acid as a functional monomer, gamma-methacryloxypropyl trimethoxy silane as a cross linker, 96-microtitor plate as a carrier and methylbenzene as a pore-foaming agent. The invention has the advantages of low cost, simple synthesis process and easily-controlled reaction conditions; and the prepared ractopamine bionic antibody can be used for enzyme-linked immunosorbent analysis of the ractopamine and detecting trace ractopamine in animal original foods.

Description

technical field [0001] The invention relates to the technical field of polymer materials, in particular to a preparation method of a ractopamine biomimetic antibody. Background technique [0002] Ractopamine hydrochloride (RAC) is a phenolamine beta 2 -Adrenergic agonist, has the effect of nutrient redistribution, enhances fat catabolism, promotes protein synthesis, can significantly improve lean meat rate and improve feed conversion rate. However, when the cumulative ingested dose in the human body exceeds a certain value, toxic and side effects are prone to occur. At present, in addition to the United States approved it as a feed additive for pigs, many countries such as the European Union and Japan have listed it as a prohibited drug, prohibiting its use in livestock and poultry production. In March 2002, Nongmufa [2002] No. 1 issued by the Ministry of Agriculture of the People's Republic of China clearly stipulates that the use of RAC is prohibited in the "Notice on Is...

AI Technical Summary

Problems solved by technology

Among them, the instrumental analysis method has high accuracy and sensitivity, but the instrument is relatively expensive, and the detection is time-consuming and costly; the immunoassay method has the characteristics of high efficiency, rapidity, low cost and a large amount of samples for one detection, but the probability of false positives Larger, prone to misjudgment

[0003] In the immunoassay method of RAC, since RAC is a small molecular compound, it cannot make animals produce antibodies, and it needs to be coupled with an appropriate carrier protein to induce the corresponding antibodies in immunized animals. Therefore, it is quite difficult to prepare pesticide antibodies with stable quality. , thus limiting the scope of application of immunoassays

In immunoassays, biological antibodies are sensitive to the external environment and are easily inactivated. The harsh purification conditions limit their wide application.