Rhododendron decorum tissue-culture quick propagation method

A tissue culture and rapid technology, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of difficulty in large-scale seedling raising, long cultivation period, low survival rate, etc. The effect of short, low reproduction coefficient

Inactive Publication Date: 2012-02-08
GUIZHOU INST OF BIOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, due to the limitation of resources and seasons, the existing techniques of seed propagation and cutting propagation of Rhododendron large white make it

Method used

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  • Rhododendron decorum tissue-culture quick propagation method
  • Rhododendron decorum tissue-culture quick propagation method
  • Rhododendron decorum tissue-culture quick propagation method

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Experimental program
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Embodiment Construction

[0049] The present invention will be further described below in conjunction with embodiment.

[0050] Embodiments of the present invention: when implementing a kind of rhododendron large white tissue culture rapid propagation method of the present invention, comprise the rapid propagation method that adopts the cotyledon of seed embryo and hypocotyl to form cluster bud, its process is made up of following steps:

[0051] a. Preparation of explants

[0052] Take the uncracked capsules of Rhododendron maxima, scrub the surface of the capsules with a brush under running water, disinfect with 75% ethanol for 30 seconds on the ultra-clean workbench, disinfect with 0.1% mercury liter solution for 10 minutes for inoculation, rinse once with sterile water, and then rinse with sterile water for 10 seconds. Soak in % hydrogen peroxide solution for 3-5 minutes, rinse with sterile water for 5-6 times, and finally dry the surface with sterile filter paper, and set aside;

[0053] b. Estab...

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Abstract

The invention discloses a rhododendron decorum tissue-culture quick propagation method, and particularly relates to a quick propagation method which adopts a tissue culture technique to carry out the callus induction to form caespitose shoots which are differentiated to a complete plant. The rhododendron decorum tissue-culture quick propagation method comprises the following steps: a. preparation of explants; b. establishment of aseptic culture system; c. induction and propagation culture of caespitose shoots; d. elongation of the caespitose shoots and sound seedling culture; e. rooting induction culture of test-tube plantlets; and then after being cut into a single plant, each caespitose shoot with the height of 2cm to 3cm is inoculated onto rooting culture medium, the culture condition is that the culture temperature is 25 DEG C plus or minus 1, illumination time is 12h.d-1, and the illumination strength is 40mol.m-2.s-1 to 60 mol.m-2.s-1; the caespitoes shoot begins to rooting after 50 days, the rooting rate is 60 percent to 65 percent, the height of the seedling can reach 4cm to 5cm after 70 days, the quantity of the root can reach 2 to 3, and the complete plant is transplanted into substrate which is the mixture of yellow sand, perlite and humus soil to be processed in domestication culture. The rhododendron decorum tissue-culture quick propagation method has the advantages that the culture period is shortened, the method is free from being influenced by the season, the survival rate is high, the yield is high and the like.

Description

technical field [0001] The invention relates to a rapid propagation method of rhododendron maxima of wild alpine rhododendron species, belonging to the technical field of flower cultivation. Background technique [0002] Rhododendron greater white is an evergreen woody plant of the genus Rhododendron L. in the Ericaceae family. It is a unique species in my country. It is mainly distributed in Sichuan, Yunnan, Guizhou, Tibet, Hubei, Hunan and other places. 3200m under the forest, in the thickets. Rhododendron large white, funnel-shaped bell-shaped corolla, white or rose-colored, with green or pink spots on the pedicel, when the flowers bloom, there is a burst of fragrance, refreshing, and very high ornamental value. Rhododendron grandis is also a commonly used Chinese herbal medicine in my country. Its nature and flavor are "bitter, cool, and slightly poisonous". In addition, the petals of Rhododendron dabai are also edible, rich in various amino acids and vitamins, with exq...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 刘燕陈训巫华美
Owner GUIZHOU INST OF BIOLOGY
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