Oil and fat composition containing diglyceride and use thereof
A technology for oil composition and diglyceride, applied in the field of oil and fat, can solve problems such as difficult to achieve, and achieve the effects of relieving metabolic syndrome, facilitating popularization and application, and good flavor
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Embodiment 1
[0024]Under the condition of 50% water addition (by oil weight), the pomegranate seed oil is catalyzed with free 1,3-position specific lipase (Lipase F-AP15, Amano Enzyme Inc., enzyme amount is 0.1% of the weight of the oil) at 40°C for the hydrolysis reaction of the oil, stop the reaction after the acid value of the oil sample reaches 60mg KOH / g, keep it at 65°C for 0.5h to inactivate the enzyme, then let it stand to remove most of the water, in the condition of vacuum and 80°C The remaining water was removed under the pressure, and then diatomite with an oil weight of 1% was added, and the residual enzyme protein was removed by filtration after stirring for 0.5h. Composition 1 based on esters and triglycerides and Composition 2 based on free fatty acids.
[0025] After mixing Composition 2 and glycerin (fatty acid group: glycerol=2, mol), add immobilized 1,3-specific lipase (Lipozyme RM IM, Novozymes A / S, the amount of enzyme added is oil weight 5%), while keeping the insid...
Embodiment 2
[0027] Under the condition of 30% water addition (in terms of oil weight) and strong stirring, the safflower oil is used as a catalyst (Lipozyme 20000L, Novozymes A / S, the amount of enzyme added is oil weight of 0.2%) at 45°C for the hydrolysis reaction of oil, stop the reaction after the acid value of the oil sample reaches 65mg KOH / g, keep it at 70°C for 0.5h to inactivate the enzyme activity, then let it stand to remove most of the water, under the conditions of vacuum and 80°C Remove the remaining water, then add diatomaceous earth with an oil weight of 1.5%, stir for 0.5h, filter to remove the residual enzyme protein, and then further separate the remaining product through molecular distillation, then decolorize and deodorize in a conventional manner to obtain diglyceride and triglyceride-based oil composition 4 and free fatty acid-based composition 5.
[0028] After mixing Composition 5 and glycerol (fatty acid group: glycerol=2, mol), add immobilized 1,3-specific lipase...
Embodiment 3
[0030] Cannabis oil and glycerin (cannabis oil: glycerol=2, mol) are placed in 50mL Erlenmeyer flask with stopper, after thorough mixing, add the immobilized lipase (Lipozyme RM IM of oil weight 5%, Novozymes A / S), Shake the reaction at a reaction temperature of 60°C for 8 hours. After the reaction product is filtered to remove immobilized enzyme particles, glycerin, ethyl ester and monoglyceride are further separated by molecular distillation, and the heavy fraction is then decolorized and deodorized in a conventional manner. Fat composition 7 was obtained.
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