Plant extract-containing tobacco and preparation method thereof
A plant extract, extract technology, applied in tobacco, processed tobacco, food science, etc., to achieve the effect of reducing cytotoxicity and reducing cytotoxicity
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[0019] 1. Experimental materials
[0020] 1.1 Reagents
[0021] Hydroxyl radical kit, product of Nanjing Jiancheng Bioengineering Institute, batch number: 20091218; anti-superoxide anion free radical kit, product of Nanjing Jiancheng Bioengineering Research Institute, batch number: 20100122; Anthracene (99%), American ACROS ORGANICS Product; DPPH, thiazolium blue (tetramethylazozoline salt, MTT), product of SIGMA Company of the United States; citric acid, Beijing Chemical Reagent Company; ascorbic acid (L-Ascorbic acid, C 6 h 8 o 6 ), p-aminobenzenesulfonic acid, α-naphthylamine, Na 2 HPO 4 12H 2 O, sodium nitrite, dimethylamine aqueous solution (33%), and sodium carbonate decahydrate are all products of Sinopharm Chemical Reagent Co., Ltd.; absolute ethanol, acetone, and acetic acid (glacial acetic acid) are all domestic analytical grade.
[0022] 1.2 Test article
[0023] Total flavonoids of licorice, products of Henan Beite Biotechnology Co., Ltd.; total saponins of ...
Embodiment 1
[0058] Weigh 10 mg of total saponins of Ophiopogon japonicus into a sterile container, add 50 ml of 50% ethanol, stir to dissolve completely; pass the solution through a microporous membrane; then make up 50% ethanol to volume. Spray the filtrate evenly on 1kg of tobacco to obtain the product.
Embodiment 2
[0060] Weigh 10 mg of total astragalosides into a sterile container, add 50 ml of 65% ethanol, stir to dissolve completely; pass the solution through a microporous membrane; then make up 65% ethanol to volume. Spray the filtrate evenly on 1kg of tobacco to obtain the product.
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