Airlift type photo-biologic reactor capable of on-line regulation and control of light intensity

Abstract

The invention relates to an airlift type photo-biologic reactor capable of on-line regulation and control of light intensity. The reactor comprises a light source glass cylinder and a guide cylinder body which are arranged in a reactor barrel, a radiation instrument interface assembly arranged on the side wall of the reactor barrel, and a light regulating and controlling system arranged outside the reactor, wherein the light source glass cylinder is embedded outside a guide cylinder in an annular space; the guide cylinder body is connected with an upper cover and the light source glass cylinder into a whole and adopts a mechanical rail lift mode; the radiation instrument interface assembly can be cooperated with a spherical radiation instrument for use; and the light regulating and controlling system is used for on-line regulation and control of LED (light-emitting diode) light source light intensity through an intelligent controllable direct-current power supply capable of performing PLC (programmable logic controller) control. On the basis of meeting sterile culture, the reactor meets the requirement of microalgae growth on light intensity and light-dark circulation, thereby solving the light intensity monitoring problem in the photo-biologic reactor culture process and achieving the effective monitoring and automatic regulation and control of light in the photo-biologic reactor culture process. The reactor provided by the invention is suitable for industrial production on large scale.

Description

technical field [0001] The invention relates to a bioreactor, in particular to an air-lift photobioreactor for on-line regulation of light intensity for closed microalgae cultivation. Background technique [0002] Microalgae are single-celled algae with small size, simple structure and rapid growth. They are widely distributed, from tropical, temperate to frigid regions to the north and south poles, from freshwater lakes, saline marshes to seawater, from the water surface to the depths of the ocean. The presence and reproduction of microalgae. Many microalgae have important economic value. For a long time, people have only used microalgae as direct or indirect live bait for fish, shrimp, shellfish larvae or adults. In recent decades, especially after the 1980s, microalgae biotechnology has developed rapidly. People have realized that microalgae are multi-sourced and genetically diverse, and that algal cells contain a variety of special secondary metabolites. , In addition...

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Problems solved by technology

From the existing literature reports and patent applications, it can be seen that the main factors that restrict its scale-up to industrialization include the following factors: ①Most airlift photobioreactors use transparent materials such as glass and plexiglass, and are intended to meet the requirements of internal and external lighting. The growth of microalgae requires light, but the pressure resistance of plexiglass is limited, and the pressure resistance of the glass cylinder after enlargement is also significantly weakened, which has caused obstacles to the online high-temperature disinfection and sterilization of the reactor after enlargement; ② some airlift The photobioreactor introduces the design of high-temperature sterilizable stainless steel tank and built-in light source, but the design of the light source follows the design of the transparent tank reactor, and has not been effectively improved to meet the needs of microalgae growth;③ Existing photobioreactors are usually unable to measure the internal light intensity, and the adjustment of the light intensity of the photobioreactor can only be performed manually by relying on cultivation experience or multiple optimization results. It is even more difficult to realize the automatic online regulation by combining light intensity measurement and light intensity adjustment to meet the light intensity requirements of microalgae, which directly limits the industrialization process of photobioreactor amplification.

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