Biological fermentation process-oriented olfactory analog instrument and online analysis method

Abstract

The invention discloses a biological fermentation process-oriented olfactory analog instrument and a biological fermentation process-oriented online analysis method. The biological fermentation process-oriented instrument is characterized by consisting of a test box and a computer; a gas-sensitive sensor array is arranged in a constant-temperature box; in the constant-temperature process, the constant-temperature box is heated only, but not refrigerated; under the control of the computer, fermentation tail gas is precisely and automatically sampled by a miniature vacuum pump, an electromagnetic valve, a flowmeter, a throttle valve and a tail gas bottle, so as to realize the online detection of the biological fermentation process; a modularized time-varying neural network and a modularized non-time-varying neural network form a cascade model to realize the online analysis of the biological fermentation process; the online analysis method comprises judgment whether the fermentation process is normal or not; recognition of tail gas of O2, NH3 and CO2, alcohols, acids, esters and other components and concentration evaluation; prediction of oxygen dissolved in a fermentation liquor, a pH value and a respiratory quotient, evaluation on concentrations of a substrate, a thallus and a product; and optimization of a material supplementing amount, a gas inflating amount and a stirring speed. By the online analysis method, the real-time dynamic display of the biological fermentation process and Internet network remote monitoring are realized.

Description

technical field [0001] The present invention is an olfactory simulation instrument and online analysis method for biological fermentation process, which relate to the fields of computer, precision measurement, automatic control, precision machinery, bioreactor, fermentation engineering and analytical chemistry. The invention mainly solves the problems of precise constant temperature of the gas sensor array studio, precise automatic sampling of fermentation tail gas, design of olfactory simulation instrument and its connection and communication with the fermentation tank, online detection and remote transmission and visualization of the biological fermentation process, state prediction of the biological fermentation process, control and optimization problems. Background technique [0002] Modern fermentation engineering is a bridge for the industrialization of genetic engineering, enzyme engineering, and cell engineering. The online detection, control and optimization technol...

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Problems solved by technology

[0017] Since the fermentation process takes a long time and the content of various organic components in the exhaust gas is very small, the data acquisition card should have sufficient sampling accuracy and resolution in terms of hardware; , it is necessary to study a macro-continuous and micro-magnified data recording and online display method to realize dynamic partial zoom-in display and remote transmission of data; problems to be solved for fermentation state visualization include visualization of individual gas sensor response; overall response of gas sensor array Visualization; Exhaust O 2 , CO 2 , NH 3 Visualization of components such as , ethanol, and lactic acid; visualization of the concentrations of substrates, bacteria, and products

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