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Pouzolzia extract, preparation method thereof, and application thereof

A technology of water extract and Pueraria japonica, which is applied in the field of medicine, can solve the problem that the medicinal value of Pueraria japonica has not been fully developed and utilized, the preparation and application of pus-pulling and anti-inflammatory extracts have not been seen, and the pharmacological and medicinal components have not been seen. Report and other issues, to achieve the effect of good application prospects and industrial practical value, simple preparation method, rich raw material resources

Inactive Publication Date: 2012-02-29
GUANGDONG PHARMA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] There are not many research reports on Pueraria genus at home and abroad, mainly sporadic reports on the antibacterial, anthelmintic, anti-tumor and blood sugar-lowering applications of medicinal materials. There is no report on its active ingredients, nor the preparation and application of its extract related to pus extraction and anti-inflammation.

Method used

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  • Pouzolzia extract, preparation method thereof, and application thereof
  • Pouzolzia extract, preparation method thereof, and application thereof
  • Pouzolzia extract, preparation method thereof, and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] The preparation of embodiment 1 extract

[0057] Take 100g of Mishuige whole grass, remove impurities, cut into pieces, add 1500mL of distilled water, heat and reflux to extract 3 times, each time 2h, combine the 3 extracts, concentrate into extract, add water to volume 100mL, and make a concentration of 1g of crude drug / mL of liquid medicine; 50mL of the liquid medicine was taken as the total extract (P) of Pueraria mist water, and the other 50mL was dispersed with a small amount of water, and extracted with chloroform, ethyl acetate, and n-butanol in sequence until the color of each extract was light, and the combined The extract (chloroform extract PC, ethyl acetate extract PE, n-butanol extract PB) and the last water part (PW) were concentrated under reduced pressure to liquid extract.

Embodiment 2

[0058] The preparation of embodiment 2 extract

[0059] Take 10kg of Pueraria japonica, remove impurities, cut into pieces, add 150L of distilled water, heat and reflux 3 times, 2 hours each time, combine the extracts 3 times, concentrate into an extract, disperse with a small amount of water, and wash with chloroform, ethyl acetate, Extract with n-butanol until the color of each extract is light, and concentrate each extract under reduced pressure to liquid extract.

[0060] Take the extract from the chloroform extraction part and carry out silica gel (100-200 mesh) column chromatography separation, chloroform:methanol (300:1-1:1, volume ratio) gradient elution, and combine similar fractions after detection by thin-layer chromatography. Obtained 7 components Fra.A-G. Take fraction Fra.B and proceed to silica gel (300-400 mesh) column chromatography (mass ratio of sample to silica gel is 1:50), and gradient elution with chloroform-methanol (200:1-20:1, volume ratio), 34 frac...

Embodiment 4

[0074] The experiment of embodiment 4 extracts to mouse subcutaneous abscess

[0075] (1) Experimental materials: erythromycin ointment (Guangzhou He Jigong Pharmaceutical Factory, batch number: S1019); Pulling pus ointment (hospital preparation of Foshan Hospital of Traditional Chinese Medicine, batch number: 100921); 10% formaldehyde fixative (Shandong Tuobo Plastic Co., Ltd., Lot No. 100226); Mishui Kudzu Extract, homemade.

[0076] (2) Experimental animals: SPF grade NIH male mice (body weight 18-22 g), purchased from the Medical Experimental Animal Center of Guangzhou University of Traditional Chinese Medicine, certificate number: SCXK (Guangdong) 2008-0020.

[0077] (3) Experimental method: select 10% (mass percentage concentration) starch aqueous solution as the external liniment matrix, add each liquid extract in Example 1 into the matrix respectively, so that the total volume is 50mL, and prepare Mishui Geshui External liniment for each extraction part of the extract. ...

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Abstract

The invention discloses a pouzolzia extract, a preparation method thereof, and an application thereof. According to the invention, for a first time, four compounds are separated and identified from subsiding swelling and pus removing effective parts of pouzolzia. A water extract prepared from pouzolzia and a chloroform or n-butyl alcohol extracted part thereof provide subsiding swelling and pus removing effects. The chloroform extracted part contains friedelin. The n-butyl alcohol extracted part contains kaempferol-7-O-alpha-L-pyran rhamnoside, quercetin-7-O-alpha-L-pyran rhamnoside, quercetin-3, and 7-O-alpha-L-2-pyran rhamnoside. The extract provided by the invention provides good subsiding swelling, pus removing, inflammation resisting, and pain relieving effects. The extract can be used for preparing subsiding swelling and pus removing medicines.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to a preparation method and application of an extract of Pueraria japonica. technical background [0002] According to "Xinhua Compendium of Materia Medica", there are four medicinal plants of the genus Pouzolzia in the Urticaceae family, including Pouzolzia elegans Wedd., Pouzolzia zeylanica (L.) Benn. Pouzolzia sanguinea(Bl.)Merr. and Pouzolzia zeylanica(L.)Benn.Var.microphylla(Wedd.)W.T.Wang. Both have the effects of dehumidification, swelling and detoxification. Mishui kudzu plants are rich in resources and are widely used among the people. They are also known as Dixiao, Pujianxiao, Chupupao, Shizhuzi, etc. Its main functions are detoxification, swelling, and bone setting. road infection etc. The ancient book "Minnan Folk Herbal Medicine" records: Mishuige has a strong effect in pulling out pus, and it should not be used for those without pus, so as not to increas...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/185A61K31/56A61K31/7048C07J63/00C07H17/07C07H1/06A61P29/00A61P17/02
Inventor 郭丽冰邓琪陈艳芬严优芍
Owner GUANGDONG PHARMA UNIV
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