Anti-herpes simplex virus (HSV) active polypeptide of Tibetan Pi scorpion and application thereof

A technology of herpes simplex virus and Tibetan scorpion, applied in the field of oral herpes simplex, genital herpes, Tibetan scorpion anti-herpes simplex virus active polypeptide, herpes zoster topical gel, in the field of treatment and treatment of herpes simplex, and can solve problems such as poor curative effect , to achieve the effect of good curative effect, obvious effect and obvious therapeutic effect.

Inactive Publication Date: 2012-03-21
WUHAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, the use of these antiviral drugs produces drug resistance of the virus, and most of t

Method used

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  • Anti-herpes simplex virus (HSV) active polypeptide of Tibetan Pi scorpion and application thereof
  • Anti-herpes simplex virus (HSV) active polypeptide of Tibetan Pi scorpion and application thereof
  • Anti-herpes simplex virus (HSV) active polypeptide of Tibetan Pi scorpion and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1: Preparation of a Tibetan lute scorpion anti-herpes simplex virus polypeptide gene.

[0033] Proceed as follows:

[0034] A: Extraction of total RNA from Tibetan lute scorpion venom glands (Trizol LS one-step method: Trizol LS was purchased from Invitrogen, USA)

[0035] ①Take 500mg of scorpion gland and grind it into fine powder in liquid nitrogen, add 10ml TRIZOL reagent and mix well, and leave it at room temperature (20-25℃, the same below) for 5 minutes; Minutes, centrifuge at 12,000g for 15 minutes at 4°C; ③Add 1 volume of isopropanol to the water phase, place at room temperature for 10 minutes, and centrifuge at 12,000g for 10 minutes at 4°C to obtain RNA precipitation; ④Wash the precipitate with 5m175% ethanol, and centrifuge at 7,500g for 5 minutes; ⑤ After the RNA precipitate is dried, dissolve it in DEPC-treated water, and incubate at 55-60°C for 10 minutes to completely dissolve the RNA. The whole process was carried out according to the method re...

Embodiment 2

[0058] Example 2: Structural analysis of AHSVP1 polypeptide and its homologous amphipathic polypeptide

[0059] According to the mature peptide sequence (FIRKIARLLKRIF) of AHSVP1, the online NPSserver [DSC method (Discrimination of protein Secondary structure Class)] was used to predict its secondary structure, and the software AHTHEPROT 2000 was used to display its secondary structure image. The results showed that AHSVP1 contained 100% α-Helix structure, which had a typical amphiphilic α-Helix structure and contained a large number of basic residues (Arg and Lys) with net positive charges. According to the spiral image figure 2 , and then carried out a large number of point mutations to the AHSVP1 polypeptide sequence FIRKIARLLKRIF (Table 1), and found that FIX1X2IAX3LLX4X5IF (X1, X3 and X5 are arbitrary basic amino acids; X2 and X4 are arbitrary amino acids) (SEQ ID NO: 3) sequence does not affect its parent sexual characteristics ( figure 2 ).

Embodiment 3

[0060] Example 3: Chemical synthesis of AHSVP1 polypeptide and its homologous amphipathic polypeptide

[0061] Artificially synthesized according to the amino acid sequences of AHSVP1 and its homologous amphipathic polypeptides (FIRKIARLLKRIF and FIX1X2IAX3LLSX4IF). The method of solid-phase chemical synthesis has obtained high-purity AHSVP1 polypeptide ( image 3 and Figure 4 ) and its homologous structure amphipathic polypeptide (Table 1).

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Abstract

The invention discloses an anti-HSV active polypeptide of Tibetan Pi scorpion and application thereof. According to the invention, through the methods of molecular biology and chemical synthesis, the anti-HSV active polypeptide of Tibetan Pi scorpion, AHSVP1, is obtained; the antiviral activity of AHSVP1 to HSV is determined by using the method of plaque array. The invention also discloses externally used anti-HSV gel which is prepared from 0.5 wt % of the medicine AHSVP1 and 1.5 wt % of a carboxymethylcellulose matrix. The gel provided in the invention is used for anti-HSV infection; the products of the gel are stable and are suitable for storing and transporting. A production process for the gel has the advantages of simple and reasonable design, low production cost and suitability for mass industrial production.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a Tibetan lute scorpion anti-herpes simplex virus active polypeptide. The invention also relates to an external gel for treating herpes simplex, herpes labialis, genital herpes and herpes zoster. Background technique [0002] Herpes simplex virus (HSV) is a common human infectious disease virus, mainly infecting the skin and mucous membranes of the mouth, eyes, lips, central nervous system, genitals, internal organs, etc., causing herpes labialis, encephalitis, keratoconjunctivitis, genital diseases such as herpes. The treatment of herpes is mostly external medicine, internal medicine, or a combination of internal medicine and external medicine, and physical methods are also used for treatment, but the overall treatment effect is not ideal, and there are many adverse reactions after use. [0003] Currently herpes is mainly treated with antiviral drugs. For example, acyclovir (ACV) i...

Claims

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Application Information

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IPC IPC(8): C12N15/12C07K7/08A61K38/10A61K9/00A61K47/38A61P31/22
Inventor 李文鑫曹志贱吴英亮
Owner WUHAN UNIV
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