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Apparatus for integrated real-time nucleic acid analysis, and method for detecting a target nucleic acid using same

An integrated device and nucleic acid analysis technology, which is applied in the field of real-time nucleic acid analysis integrated devices, can solve the problems of 96-well nucleic acid amplification reactor waste, economic time limit, wasteful device operation, etc., and achieve shortened test time, efficient use, and saving The effect of fees

Active Publication Date: 2012-03-21
BIONEER
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] As in the case above, it takes a long time until a plurality of samples of the same type are obtained, and using the existing 96-well nucleic acid amplification reactor to analyze only a few hepatitis B detection samples will generate a huge waste
At this point, you end up wasting a run of the device or waiting days until a test sample is available
For this reason, there is a problem that, in a small-to-medium-sized hospital, when various clinical tests are performed with a relatively small number of clinical samples, there are economic and time constraints, and when entrusting an external testing agency Requires more time, expense and effort

Method used

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  • Apparatus for integrated real-time nucleic acid analysis, and method for detecting a target nucleic acid using same
  • Apparatus for integrated real-time nucleic acid analysis, and method for detecting a target nucleic acid using same
  • Apparatus for integrated real-time nucleic acid analysis, and method for detecting a target nucleic acid using same

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Embodiment 1

[0060] Embodiment 1 relates to the real-time nucleic acid analysis integrated device of the present invention, figure 1 A schematic diagram of the real-time nucleic acid analysis integrated device of the present invention is shown, and the present invention will be described in more detail below.

[0061] The integrated real-time nucleic acid analysis device includes a plurality of automatic purification and dispensation devices 100 for separating and purifying different nucleic acids from multiple types of biological samples containing nucleic acids. The above-mentioned biological sample is a biological substance isolated in a natural environment and containing nucleic acid. The biological sample of the present invention may be an isolated or purified nucleic acid solution, microorganism, tissue, biological fluid or cell. Biological fluids include, but are not limited to, blood, plasma, sputum, urine, cerebrospinal fluid, gastric washings, leukophoresis, and various specimen...

Embodiment 2

[0106] Embodiment 2 relates to the target nucleic acid detection method using the real-time nucleic acid analysis integrated device of the present invention, which will be described in more detail below.

[0107] The present invention provides a target nucleic acid detection method using a real-time nucleic acid analysis integrated device, which is characterized in that it comprises:

[0108] 1) In a plurality of automatic purification and dispensing devices 100, the nucleic acid contained in each sample is separated and purified from the standard sample and various biological samples containing nucleic acid, and then the separated and purified solution is added to the nucleic acid amplification reaction mixture The step of mixing the reaction tube;

[0109] 2) By making the porous temperature circulation block 210 in which the reaction tube of the nucleic acid amplification reaction mixture is installed correspond to each target nucleic acid separated and purified from the au...

Embodiment 3

[0159] Example 3 relates to the internal control for the separation and purification of nucleic acid and the simultaneous detection of multiple samples in the target nucleic acid detection method using the integrated real-time nucleic acid analysis device of the present invention. The present invention will be described in more detail below.

[0160] (1) Design and application of plant internal control primers and probes

[0161]Plant internal control (Internal Positive Control, IPC) is the base sequence 4903 to 5709 (Movement protein gene, MP) in the genome RNA (GeneBank.ACCESSION No.NC001367) of tobacco mosaic virus (Tobacco mosaic virus, TMV), Between 5712 and 6191 (Coat protein gene, CP), base sequences with a length of 18-28 bp and a Tm value of 55° C. to 62° C. were randomly selected as forward and reverse primers.

[0162] In addition, among the above-mentioned base sequences, base sequences with a length between 19 and 30 bp and a Tm value between 67 and 72°C were rand...

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Abstract

The present invention relates to an integrated real-time nucleic acid analysis apparatus and to a method using same. More particularly, the present invention relates to an integrated real-time nucleic acid analysis apparatus which performs a qualitative analysis and a quantitative analysis at the same time on genes corresponding to a plurality of biological samples of various types, and to a method for detecting a target nucleic acid using same. The integrated real-time nucleic acid analysis apparatus and the method for detecting a target nucleic acid using same according to the present invention perform just a single test on a variety of targets, requested for a variety of analytes, in a quick and accurate manner, and thus can be efficiently used in a hospital or the like where diagnoses for diseases are required.

Description

technical field [0001] The present invention relates to a real-time nucleic acid analysis integrated device and a target nucleic acid detection method using the device, and in more detail, to a real-time nucleic acid that simultaneously performs qualitative or quantitative analysis of genes corresponding to the samples from a plurality of biological samples of various types An analysis integrated device and a target nucleic acid detection method using the device. Background technique [0002] In vitro diagnostic testing (in vitro diagnostic testing: IVD testing) is for the purpose of determining whether a disease is infected, etc., using samples from the human body such as blood, urine (urine) and saliva (saliva) as a sample to detect specific indicator substances A test for detection or quantitative analysis. Molecular diagnostic testing (Molecular diagnostic testing) or nucleic acid diagnostic testing (NAT; nucleic acid testing) is the fastest growing field in the in vitr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12M1/38C12N15/09
CPCB03C1/30B03C1/288G01N35/0098B03C1/01C12Q1/6844B03C2201/26Y02A90/10C12Q2561/113C12Q2537/143G16B99/00C12M1/38C12Q1/6851
Inventor 金裕晶丘完林金锺勋张大镇徐振喆金成烈朴海埻朴翰浯
Owner BIONEER