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Preparation method of agar gel microspheres

An agar gel and microsphere technology, which is applied in the fields of biochemical separation of bioengineering and cells and their protein carriers, can solve the problems of difficulty in realizing industrialized production, complicated operation process and high production cost, and achieves improved uniformity and simple preparation process. , the effect of reducing production costs

Active Publication Date: 2012-03-28
BEIJING UNIV OF CHEM TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

This patented technology has high requirements on the membrane, and needs to add several supporting equipment. The operation process is complicated, the equipment is high, the production cost is also high, and it is difficult to realize industrial production.

Method used

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  • Preparation method of agar gel microspheres
  • Preparation method of agar gel microspheres
  • Preparation method of agar gel microspheres

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Prepare 200ml of 10wt% agar aqueous solution by heating, and at a temperature of 70°C, the initial stirring speed is 4500rpm, slowly add the agar aqueous solution to 600ml of 84v% cyclohexane and 16v% Span 85 press Mixed composition of the oil phase. During the emulsification process, adjust and increase the stirring speed so that the final stirring speed is 8000rpm, and continue to stir for 15 minutes, and then cool the emulsion to room temperature by program cooling, and maintain the final stirring speed during the cooling process. Wash the prepared agar gel bare ball with water, 50% ethanol, and water successively, and use OLYMPUS CX41 ( olympus co., ltd. , Japan) optical microscope to observe the product morphology, the results are as follows figure 1 Shown; Adopt MASTERSIZER 2000 laser particle size analyzer (Malvern Instrument Company, UK) to analyze the particle size of product, the result is as follows figure 2 shown. From figure 1 It can be seen from the ...

Embodiment 2

[0039] Prepare the same agar aqueous solution as in Example 1, at a temperature of 72° C., under the condition of 5000 rpm at the initial stirring speed, slowly add the agar aqueous solution to 800 ml of 82v% hexanaphthene and 18v% Span 85 mixture while hot. in the oil phase. During the emulsification process, the stirring speed was adjusted and increased so that the final stirring speed was 9500 rpm, and the stirring was continued for 20 minutes. The obtained product was cooled and washed by the same method as in Example 1, and the product shape was observed and the particle size of the product was analyzed. The results showed that the 10% agar gel bare spheres prepared by this method had a regular spherical shape, and the volume fraction of the gel bare spheres with particle diameters ranging from 30 to 90 μm was 81.2%.

[0040] The cross-linking process of the agar gel bare sphere is different from Example 1 in that the stirring speed is 180rpm, and the Na 2 SO 4 Dosage i...

Embodiment 3

[0043] Prepare the same agar aqueous solution as in Example 1, at a temperature of 68° C., under the condition that the initial stirring speed is 1100 rpm, the agar aqueous solution is slowly added to 400 ml of 86v% hexanaphthene and 14v% Span 85 mixture while hot. in the oil phase. During the emulsification process, the stirring speed was adjusted and increased so that the final stirring speed was 1800rpm, and the stirring was continued for 25 minutes. The obtained product was cooled and washed by the same method as in Example 1, and the product shape was observed and the particle size of the product was analyzed. The results showed that the 10% agar gel bare spheres prepared by this method had a regular spherical shape, and the volume fraction of the gel bare spheres with particle diameters ranging from 450 to 600 μm was 80.5%.

[0044] The cross-linking process of the agar gel bare sphere is different from Example 1 in that the stirring speed is 140rpm, and the Na 2 SO 4 ...

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Abstract

The invention relates to a preparation method of agar gel microspheres. Through the method, the uniformly spherical agar gel microspheres with even grain diameter which contain 4-20wt% of agar, and are controllable in the range of 1-900 mum are prepared through the processes of taking the agar as the raw material, preparation of oil phase and aqueous phase, dispersed emulsion, cooling and solidifying, crosslinkage and ligand modification. The agar gel microspheres are excellent in mechanical strength, high in heat stability, strong in renewability, low in cost and can be used as separation medium. The method is simple in operation process, low in equipment requirement, low in energy consumption, and can be used for realizing industrial preparation.

Description

technical field [0001] The invention relates to the fields of biochemical separation of bioengineering and cells and their protein carriers. More specifically, the present invention relates to a kind of preparation method of agar gel microsphere. Background technique [0002] Polymer microspheres refer to polymer materials with diameters ranging from nanometers to micrometers and spherical or other geometric shapes. At present, gel microspheres using natural polymers as raw materials mainly include sodium alginate gel microspheres, chitosan gel microspheres, dextran gel microspheres and agarose gel microspheres. Among them, agarose gel microspheres are mainly used as a chromatographic medium for the separation of proteins, peptides, nucleic acids, and sugars, and can also be used as living cells or drug carriers. But the current market price of agarose gel is relatively expensive. For example, the price of sepharose 6B is about 700 yuan / 100ml, and the price of cross-linked...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J13/02
Inventor 谭天伟胡瑜杨自信魏军陈艳敏
Owner BEIJING UNIV OF CHEM TECH
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