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Preparation method for microencapsulated oral live vaccine of gosling plague

A technology of microencapsulation and gosling plague, applied in the field of bioengineering, can solve the problems of inconvenient use, affecting the production performance of breeding geese, short immunization period, etc., and achieve the effects of convenient use, less loss of vaccine potency and long storage period

Inactive Publication Date: 2013-04-17
HANGZHOU JIANLIANG VETERINARY BIOLOGICAL PREPARATIONS CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The present invention makes full use of the excellent characteristics of the microcapsules to solve the deficiencies of the current gosling plague freeze-dried live vaccine, such as short immunity period, inconvenient use, and affecting the production performance of breeding geese, and can perform oral immunization during the egg-laying period of breeding geese. no stress response

Method used

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  • Preparation method for microencapsulated oral live vaccine of gosling plague
  • Preparation method for microencapsulated oral live vaccine of gosling plague

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] The gosling plague SYG strain purchased from the China Veterinary Drug Control Institute was multiplied and cultured through goose embryos to make a semi-finished vaccine. After passing the sterility test, 5% sucrose skim milk solution was added, and 20% porous starch was added after mixing. , 37 ° C temperature controlled stirring for 40 minutes. The above liquid is mixed with an equal volume of 1% sodium alginate solution, fully stirred, and then dehydrated and dried in a fluidized bed to prepare a dry powder of gosling plague microencapsulated vaccine, with a particle size range of 5-15 μm. The prepared vaccine was inoculated into non-immune goose embryos, and the poison price per gram of vaccine was determined to be 1.5×10 7 ELD 50 . The vaccine was added to the diluent containing thickener and mixed well, and 50 healthy adult geese were immunized through the drinking water route, and another 30 healthy adult geese were set as the non-immune control group, and the...

Embodiment 2

[0016] The gosling plague SYG strain purchased from the China Veterinary Drug Administration was multiplied and cultured through goose embryos to make a semi-finished vaccine. After passing the sterility test, 5% sucrose skim milk solution was added, and after mixing, 40% porous starch was added. , 37°C temperature controlled stirring for 20 minutes. The above liquid is mixed with an equal volume of 2.5% sodium alginate solution, fully stirred, and then dehydrated and dried in a fluidized bed to prepare a dry powder of gosling plague microencapsulated vaccine, with a particle size range of 5-15 μm. The prepared vaccine was inoculated into non-immune goose embryos, and the poison price per gram of vaccine was determined to be 0.7×10 7 ELD 50 . The vaccine was added to the feed and mixed evenly, and 45 healthy adult geese were immunized by the mixing method, and another 30 healthy adult geese were set as the non-immune control group, and the two groups of healthy adult geese w...

Embodiment 3

[0018] The vaccine prepared according to the preparation method described in Example 1 was used to immunize 40 breeding geese through drinking water, and another 30 healthy adult geese were used as a non-immune control group. Take 10 goslings hatched from the eggs of the immunization group and the control group at different time periods (20-30 days, 31-40 days, 41-50 days, 51-60 days) after immunization, and at the age of 5 days, 10 goslings were hatched. Attack lethal dose of gosling plague virus liquid and observe for 10 days. Results The immune protection rates of different time periods in the test group were 90%, 100%, 100% and 80% respectively, while the mortality rates of the control group were 100%, 80%, 90% and 90% in different time periods. It can be seen that the vaccine has a good protective effect on goslings.

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Abstract

The present invention discloses a preparation method for a microencapsulated oral live vaccine of gosling plague. The preparation method comprises: preparing a gosling plague SYG strain into a vaccine half finished product by goose embryo proliferation culture; adding a 5% sucrose and skimmed milk solution after a sterility test is qualified, and uniformly stirring; adding 20-40% porous starch tothe half finished product solution, stirring for 20-40 minutes at a certain temperature, wherein the temperature is controlled to 37 DEG C; mixing the resulting liquid and a 1-2.5% sodium alginate solution, completely stirring, and then carrying out dehydration and drying by a fluidized bed to prepare the microencapsulated vaccine dry powder of the gosling plague, wherein the volume of the resulting liquid is the same as the volume of the sodium alginate solution. The method of the present invention ha characteristics of science, simpleness, stable and reliable production, less loss of vaccine titer. With adopting the vaccine microencapsulation technology, the live vaccine of the gosling plague can be adopted for immunization by the oral route, the stress reaction is reduced, the sustained release function is provided for the live vaccine of the present invention, and the breeder goose in the laying period can use the live vaccine as usual.

Description

technical field [0001] The invention relates to a preparation method of goose plague microencapsulated oral live vaccine, which belongs to the technical field of bioengineering. Background technique [0002] Gosling plague is a severe infectious disease caused by gosling parvovirus, which mainly affects goslings and muscovy ducks. The morbidity and mortality are very high, and the mortality rate of goslings can reach 90% to 100%. For a long time, gosling plague vaccine has been the main measure to prevent gosling plague. Though my country has the freeze-dried product of the live vaccine that prevents gosling plague at present such as gosling plague live vaccine (SYG strain) and gosling plague live vaccine (GD strain) etc., there are still some deficiencies, such as vaccine must be injected The use of this method is not only time-consuming and laborious, but also can not avoid various severe stress reactions caused by artificial rounding up and vaccination, such as: food redu...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K39/23A61K9/50A61P31/20
CPCY02A50/30
Inventor 龚建森刘学贤吕晓娟戴有理李新华徐步
Owner HANGZHOU JIANLIANG VETERINARY BIOLOGICAL PREPARATIONS CO LTD
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