Fiber bracket containing growth factors and preparation method thereof

A technology of growth factors and fiber scaffolds, applied in cellulose/protein conjugated artificial filaments, conjugated synthetic polymer artificial filaments, medical science, etc., can solve the problems of sudden release and effective life reduction, and achieve the goal of maintaining biological The effect of activity, slowing down burst release and improving encapsulation efficiency

Inactive Publication Date: 2012-04-04
WUXI ZHONGKE GUANGYUAN BIOMATERIALS
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  • Abstract
  • Description
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AI Technical Summary

Problems solved by technology

Directly dispersing bioactive substances in polymer solution by electrospinning can spin bioactive subst

Method used

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Examples

Experimental program
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Example Embodiment

[0019] Example 1:

[0020] Prepare growth factor-containing fiber scaffolds as follows:

[0021] (1) Preparation of growth factor solution: basic fibroblast growth factor was dissolved in cyclodextrin-phosphate buffer to prepare a growth factor solution with a concentration of 50 mg / ml.

[0022] (2) Preparation of polymer solution: the polymer material polylactic acid-glycolic acid copolymer is dissolved in chloroform solvent to prepare a polymer solution with a concentration of 17% by mass;

[0023] (3) Preparation of a polymer solution containing growth factors: drop the growth factor solution into the polymer solution, ultrasonicate in an ice bath, and mix uniformly to obtain an electrospinning solution.

[0024] (4) Preparation of composite fiber material: inject the mixed solution of organic polymer and growth factor into our self-made coaxial electrospinning device, the diameter of the outer shaft is 3mm, the diameter of the inner shaft is 0.6mm, the voltage is 20KV, th...

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Abstract

The invention relates to a tissue fiber bracket and a preparation method thereof, in particular to a fiber bracket containing growth factors and a preparation method thereof. The fiber bracket consists of composite fiber material and is manufactured by a coaxial electrospinning spinning device; and the composite fiber material consists of organic polymers with the weight-average molecular weight of 5-500000 and growth factors, and an organic polymer solution and a growth factor solution are mixed and then electrostatically spun to obtain the composite fiber material. The invention has the beneficial effects that the fiber bracket is prepared according to the method disclosed by the invention, the composite fiber material in a nanometer structure is prepared by the coaxial electrostatic spinning method, fiber in a nuclear shell structure can be prepared, the encapsulation rate of electrostatically spun fiber is improved, the initial burst release of the growth factors is slowed down, the bioactivity of the growth factors can be maintained for a long time, the method has a simple process and low cost, is easy to operate, and has wide application prospects in the fields of tissue engineering, wound dressing and the like.

Description

Technical field: [0001] The invention relates to a tissue fiber scaffold and a preparation method thereof, more specifically, to a fiber scaffold containing growth factors and a preparation method thereof. Background technique: [0002] Scaffolds are a major component in tissue engineering for the regeneration and restoration of damaged tissues. Tissue scaffolds should have a high degree of porosity with appropriate pore size and geometry. Therefore, fabricating nano- and micro-scale scaffolds to mimic the natural extracellular matrix (ECM) is a crucial condition for successful tissue engineering. [0003] Electrospinning is currently the only technology that can produce continuous fibers with diameters ranging from nanometers to micrometers, and the manufactured fibers are structurally similar to extracellular matrix. The porous structure of the electrospun fibrous scaffold has a very high specific surface area for cell adhesion and contains connected micropores that allo...

Claims

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Application Information

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IPC IPC(8): A61L27/48A61L27/54A61L15/32A61L15/26A61L15/24A61L15/44D01F8/14D01F8/02
Inventor 韩志超许杉杉
Owner WUXI ZHONGKE GUANGYUAN BIOMATERIALS
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