Feeding lactobacillus johnsonii high-density fermentation medium and application thereof

A technology for high-density fermentation of Lactobacillus johnsonii, applied in the fields of application, bacteria, animal feed, etc., can solve the problems of insufficient nutrients, difficulty in high-density fermentation of Lactobacillus johnsonii, and difficulty in high-density fermentation. The effect of production efficiency, shortening production cycle and reducing separation cost

Active Publication Date: 2012-04-18
BEIJING DABEINONG TECH GRP CO LTD +3
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Lactobacillus johnsoni is still difficult to achieve high-density fermentation. The main problem that is difficult to achieve is the fermentation medium and its fermentation method. The...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Preparation and fermentation comparison of embodiment 1 fermentation medium

[0039] 1. The components and content of Lactobacillus johnsonii high-density fermentation medium are:

[0040] Glucose: 20g / L, tryptone: 10g / L, beef extract: 10g / L, yeast powder: 5g / L, K 2 HPO 4 : 2g / L, CH 3 COONa: 5g / L, sodium citrate: 2g / L, MgSO 4 : 0.2g / L, FeSO 4 : 0.1g / L, MnSO 4 : 55mg / L.

[0041] 2. Medium preparation method:

[0042] (1) Weigh each component according to the above-mentioned content, dissolve with distilled water, wherein glucose is dissolved separately;

[0043] (2) Glucose is sterilized separately at 115°C for 20 minutes, and other components are put into a fermenter and sterilized at 121°C for 30 minutes;

[0044] (3) Before inoculation, glucose is added into the fermenter through aseptic operation, stirred and mixed evenly, and the obtained product is obtained.

[0045] 3. Comparison between high-density fermentation and existing technolog...

Embodiment 2

[0059] Preparation and fermentation comparison of embodiment 2 fermentation medium

[0060] 1. The components and contents of Lactobacillus johnsonii high-density fermentation medium are:

[0061] Glucose: 25g / L, tryptone: 10g / L, beef powder: 15g / L, yeast powder: 10g / L, K 2 HPO 4 : 2g / L, CH 3 COONa: 7g / L, sodium citrate: 3g / L, MgCl 2 : 0.2g / L, FeCl 2 : 0.1g / L, MnSO 4 : 50mg / L.

[0062] 2. Medium preparation method:

[0063] (1) Weigh each component according to the content, dissolve with distilled water, wherein glucose is dissolved separately;

[0064] (2) Glucose is sterilized separately at 115°C for 20 minutes, and other components are put into a fermenter and sterilized at 121°C for 30 minutes;

[0065] (3) Before inoculation, glucose is added into the fermenter through aseptic operation, stirred and mixed evenly, and the obtained product is obtained. 3. Comparison between high-density fermentation and existing technology fermentation

[0066]...

Embodiment 3

[0079] Preparation and fermentation comparison of embodiment 3 fermentation medium

[0080] 1. The components and contents of Lactobacillus johnsonii high-density fermentation medium are:

[0081] Glucose: 30g / L, tryptone: 15g / L, beef extract: 6g / L, yeast powder: 6g / L, K 2 HPO 4 : 3g / L, CH 3 COONa: 8g / L, sodium citrate: 4g / L, (NH 4 ) 2 Fe(SO 4 ) 2 : 0.2g / L, MgSO 4 : 0.4g / L, MnSO 4 : 45mg / L.

[0082] 2. Medium preparation method:

[0083] (1) Weigh each component according to the content, dissolve with distilled water, wherein glucose is dissolved separately;

[0084] (2) Glucose is sterilized separately at 115°C for 20 minutes, and other components are put into a fermenter and sterilized at 121°C for 30 minutes;

[0085] (3) Before inoculation, glucose is added into the fermenter through aseptic operation, stirred and mixed evenly, and the obtained product is obtained.

[0086] 3. Comparison between high-density fermentation and existing technol...

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Abstract

The invention belongs to the technical field of lactobacillus culture, and relates to a feeding lactobacillus johnsonii high-density fermentation medium and application thereof. By using the high-density fermentation medium and the lactobacillus johnsonii fermented by a fermentation method for the fermentation medium, the bacterial density is remarkably improved relative to common fermentation, the number of viable bacteria is not less than 1,010cfu/ml and is improved by over 80 times compared with the viable bacteria cultured by a common MRS culture medium, and high-density fermentation of the lactobacillus johnsonii is realized; and according to the fermentation medium and the application thereof, the separation cost of biomass is reduced, the production period is shortened, the production cost is reduced, and the production efficiency is improved.

Description

technical field [0001] The invention belongs to the technical field of lactic acid bacteria cultivation, and relates to a high-density fermentation medium of Lactobacillus johnsonii for feeding and an application thereof. Background technique [0002] Lactic acid bacteria are normal bacteria commonly found in the digestive tract of animals. The history of using this type of bacteria to develop probiotics is the earliest. This bacteria has been widely used in fermented food, animal feed and health food. [0003] Lactobacillus johnsonii (Lactobacillus johnsonii) is a kind of lactic acid bacteria. Based on the current literature reports about Lactobacillus johnsonii, its beneficial function to animals is mainly reflected in the following two aspects. On the one hand, Lactobacillus johnsonii has a natural defense effect on animals. System and immune function have a regulatory effect; on the other hand, Lactobacillus johnsoni showed a specific preventive effect on animal dermatit...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23K1/16C12R1/225
Inventor 孙晓雯汪攀张军闫轶洁王安如
Owner BEIJING DABEINONG TECH GRP CO LTD
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