Immunity-chromatography test paper for rapid detection of mycobacterium tuberculosis secretory protein and preparation method thereof
A technology of mycobacterium tuberculosis and immunochromatography test paper, which is applied in the field of immunochromatography, can solve the problems of high price, high pollution rate, laborious labor and so on, and achieve the effect of cheap price
Active Publication Date: 2014-04-09
ZHEJIANG UNIV OF TECH
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Problems solved by technology
Regardless of whether it is a liquid medium or an automatic culture system, there is still the problem of high contamination rate. For the samples judged positive by the instrument, smears, acid-fast staining, and acid-fast bacilli can be found under the microscope before they can be judged positive, which is time-consuming and laborious.
The automatic culture system is difficult to be popularized due to the high price of instruments and reagents
Method used
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Experimental program
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Embodiment 1
[0037] Embodiment 1, figure 2 and image 3 Combined with the preparation of a rapid detection of Mycobacterium tuberculosis secretory protein ESAT-6 immunochromatographic test paper, the following main materials are selected:
[0038] Chlorauric acid (HAuCL 4 4H 2 O), analytically pure, Sigma company;
[0039] Trisodium citrate (Na 3 C 6 h 5 o 7 2H 2 O), analytically pure, China Pharmaceutical Group Shanghai Chemical Reagent Corporation;
[0040] Goat anti-mouse IgG antibody, Shanghai Jinbiao Company;
[0041] Polyethylene glycol (PEG) 20000, analytically pure, Sigma company;
[0042] Sucrose (C12H19Cl3O8), analytically pure, Sigma company;
[0043] Bovine serum albumin (BSA), Shanghai Sangon Bioengineering Company;
[0044] Nitrocellulose (NC) membrane, Millipore;
[0045] Other commonly used reagents were of analytical grade.
[0046] step:
[0047] 1. Preparation and purification of anti-ESAT-6 mouse monoclonal antibodies TBEF3 and TBEA8
[0048] 1) Inducti...
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Abstract
The invention discloses an immunity-chromatography test paper for rapid detection of mycobacterium tuberculosis, which comprises a sample feeding pad (1), a colloidal gold pad (2) of anti ESAT-6 monoclonal antibody TBEA8 with a gold label, a cellulose nitrate membrane (5) coated with a quality control line (3) and a detection line (6), a sample suction pad (4), and a support plate (7); the detection line (6) is an anti ESAT-6 monoclonal antibody TBEF3 coated on the cellulose nitrate membrane (5), and the quality control line (3) is an sheep anti-mouse IgG antibody coated on the cellulose nitrate membrane (5); the colloidal gold pad (2) and the sample suction pad (4) are respectively disposed on the upper surface of the cellulose nitrate membrane (5), and the sample feeding pad (1) is located on the upper surface of the colloidal gold pad (2). The invention also discloses a preparation method of the immunity-chromatography test paper. The immunity-chromatography test paper is applicable to the rapid detection of mycobacterium tuberculosis in a culture at a base site.
Description
technical field [0001] The invention belongs to the technical field of immunochromatography for rapid detection of clinical microorganisms, and in particular relates to a method for early identification of Mycobacterium tuberculosis in culture by utilizing immunochromatography color development related to anti-ESAT-6 protein antibody. Background technique [0002] According to the statistics of the World Health Organization, nearly 1 / 3 of the world's population has been infected with Mycobacterium tuberculosis, and the carrier rate of Mycobacterium tuberculosis among adults in some developing countries is as high as 80%, and about 5%-10% of the carriers can be infected. develop active tuberculosis. The number of tuberculosis patients in my country ranks second in the world, and the number of people who die from tuberculosis every year is more than twice the total number of deaths from various infectious diseases. Due to the prevalence of AIDS in the past two decades, the po...
Claims
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IPC IPC(8): G01N33/577
Inventor 徐宁姚航平
Owner ZHEJIANG UNIV OF TECH