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Method for preparing blue-green algae pigment

A technology of cyanobacteria pigments and cyanobacteria, applied in chemical instruments and methods, azo dyes, organic dyes, etc., can solve the problems of cumbersome methods, environmental pollution, increase production costs, etc., and achieve the effect of high color price

Active Publication Date: 2012-05-02
陈勇
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the limitation of the enzymes used, it is still impossible to effectively extract cyanobacteria pigments and proteins and maintain the activity of cyanobacteria proteins
[0009] In addition, there are extraction methods such as chemical reagent treatment method, swelling method, ultrasonic method and tissue crushing method. These methods are all cumbersome, and the post-processing process is also relatively troublesome, which not only increases production costs, but also causes environmental pollution.

Method used

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  • Method for preparing blue-green algae pigment
  • Method for preparing blue-green algae pigment
  • Method for preparing blue-green algae pigment

Examples

Experimental program
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Effect test

preparation example Construction

[0031] The cyanobacteria used in the preparation method of the cyanobacteria pigment of the present invention has a wide range of sources, and various cyanobacteria such as spirulina, cyanobacteria, and Nostococcus can be used. The rest of the protein in the cyanobacteria pulp after the pigment is extracted will not be destroyed, and can also be used as feed protein. It does not affect the protein content, makes the best use of everything, and increases the value of by-products.

[0032] The route of the traditional extraction process of cyanobacteria pigment is too long, most of the process needs to be carried out at low temperature, and the extraction period is long, so the cost is high, the efficiency is low, and the production and application value is not high. The preparation method of the cyanobacteria pigment of the present invention uses cyanobacteria as the main raw material, combined with the deep processing of the cyanobacteria by using biotechnology methods, and the...

experiment example 1

[0035] Experimental example 1, high pressure homogeneous method

[0036] (1) Weigh 9 portions (100g each) of spirulina in 1000ml of distilled water containing 0.02M EDTA, stir and soak at 25°C for 4 hours;

[0037] (2) Homogenize the spirulina liquid under different pressures (20, 30, 40kPa) and different cycle times (1, 2, 3 times), control the homogenization speed to be 200L / h, and keep the temperature at 25°C;

[0038] (3) Centrifuge the homogeneous solution at 12,000 r / min for 20 minutes (min), remove sediment, and obtain a supernatant.

[0039] (4) Add 10 g of ammonium sulfate crystals to the supernatant, dissolve it, let stand, and precipitate crude phycocyanin.

[0040] (5) Centrifuge at 10,000 r / min for 15 minutes to obtain a precipitate.

[0041] (6) Results and analysis

[0042] The number of cycles of high-pressure homogenization has a greater impact on the extraction rate of the target product (phycocyanin) than the homogenization pressure. It can be concluded...

experiment example 2

[0045] Experimental example 2, enzymatic dissolution method

[0046] (1) Weigh 9 portions (100g each) of spirulina, dissolve them in 1000ml of distilled water containing 0.02M EDTA, stir and soak at 25°C for 4 hours;

[0047] (2) Add different total amounts (100, 200, 300 mg) of lysozyme and pectinase (lysozyme: pectinase = 1: 2) to 9 samples respectively and mix them at different temperatures (30, 40, 50 °C) at constant temperature for different time (2, 4, 6h) treatment:

[0048] (3) Centrifuge the spirulina liquid after enzymolysis for 15min under the condition of 10000r / min, and remove the sediment;

[0049] (4) Add 10g of ammonium sulfate crystals respectively in the supernatant, dissolve it, let it stand, and precipitate crude phycocyanin;

[0050] (5) Centrifuge at 10000r / min for 15min to obtain a precipitate.

[0051] From the range analysis in Table 2 below, it can be seen that the enzyme treatment temperature has the greatest impact on the target product (phycocya...

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Abstract

The invention belongs to the technical field of food additives, and provides a method for preparing blue-green algae pigment. The method comprises the steps of performing enzymolysis on blue-green algae, and performing high-pressure homogenization. The method for preparing the blue-green algae pigment specifically comprises the following steps of: performing enzymolysis on blue-green algae cells by using an enzyme preparation, crushing the blue-green algae cell walls, circulating blue-green algae cytochylema which is obtained after enzymolysis treatment in a high-pressure homogenization mode, and finally centrifuging, and performing ammonium sulfate precipitation to obtain the blue-green algae pigment. By the method, edible, high-purity, and high-valeur natural cyanine which also keeps the primary nutrition of protein of blue-green algae pigment can be obtained.

Description

technical field [0001] The invention belongs to the technical field of food additives, and relates to a preparation method of food pigments, in particular to a preparation method of cyanobacteria pigments. Background technique [0002] Food coloring includes natural coloring and synthetic coloring. Synthetic pigments have the characteristics of bright color, stable properties, and strong tinting power, but most of them come from chemical raw materials. Most of the coloring substances contain groups such as benzene rings and naphthalene rings. Ingestion of a certain amount will cause harm to the human body and even cause death. Toxic, carcinogenic and teratogenic, so there are strict restrictions on the scope and dosage of synthetic pigments, and some varieties have been banned. Edible natural pigments are colored substances extracted from the tissues of animals, plants or microorganisms. They have a natural color and a tone closer to natural food, and are easily accepted by...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09B61/00C09B67/54
Inventor 陈勇
Owner 陈勇
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