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Living nematoda egg purification and extraction agent as well as its application method

An extractant and nematode technology, applied in the field of extractants, can solve the problems of interference of residues and bacteria, large workload, low efficiency, etc., and achieve the effects of improving acquisition efficiency and quality, solving decay, and convenient operation.

Inactive Publication Date: 2014-07-30
NORTHEAST INST OF GEOGRAPHY & AGRIECOLOGY C A S
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to solve the problems of residual body and miscellaneous bacteria interference, heavy workload, long use time and low efficiency in the existing methods for obtaining nematode eggs, and to provide a living nematode egg purification extractant and its use method

Method used

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  • Living nematoda egg purification and extraction agent as well as its application method
  • Living nematoda egg purification and extraction agent as well as its application method
  • Living nematoda egg purification and extraction agent as well as its application method

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specific Embodiment approach 1

[0012] Specific embodiment 1: In this embodiment, the living nematode egg purification and extraction agent is composed of reagent A and reagent B; wherein reagent A is composed of 0.3-0.4g of sodium chloride, 1-1.5g of sodium hydroxide, 0.3-0.5g of Disodium hydrogen phosphate, 1-1.5g of sodium hypochlorite, 0.005-0.02g of magnesium sulfate, 1-1.5g of potassium hydroxide, 0.1-0.3g of potassium dihydrogen phosphate and 100mL of distilled water; reagent B consists of 0.3-0.4 gram potassium chloride, 0.2~0.3g sodium chloride, 2~3g sodium phosphate and 100mL distilled water.

specific Embodiment approach 2

[0013] Specific embodiment two: the difference between this embodiment and specific embodiment one is that the nematode ovum purification extracting agent is composed of reagent A and reagent B; wherein reagent A is composed of 0.3g sodium chloride, 1g sodium hydroxide, 0.3g Disodium hydrogen phosphate, 1g of sodium hypochlorite, 0.005g of magnesium sulfate, 1g of potassium hydroxide, 0.1g of potassium dihydrogen phosphate and 100mL of distilled water; Reagent B consists of 0.3g of potassium chloride, 0.2g of Sodium, 2g of sodium phosphate and 100mL of distilled water. Others are the same as in the first embodiment.

specific Embodiment approach 3

[0014] Specific embodiment three: the difference between this embodiment and specific embodiment one is that the live nematode egg purification extractant is composed of reagent A and reagent B; wherein reagent A is composed of 0.4g of sodium chloride, 1.5g of sodium hydroxide, 0.5 g of disodium hydrogen phosphate, 1.5 g of sodium hypochlorite, 0.02 g of magnesium sulfate, 1.5 g of potassium hydroxide, 0.3 g of potassium dihydrogen phosphate and 100 mL of distilled water; reagent B consists of 0.4 g of potassium chloride, 0.3 g sodium chloride, 3g of sodium phosphate and 100mL of distilled water. Others are the same as in the first embodiment.

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Abstract

The invention relates to a living nematoda egg purification and extraction agent as well as its application method, and solves the problems of low egg germination rate, long time requirement and low efficiency in existing nematoda egg acquiring methods. The living nematoda egg purification and extraction agent is composed of reagent A and reagent B. Specifically, the reagent A consists of sodium chloride, sodium hydroxide, disodium hydrogen phosphate, sodium hypochlorite, magnesium sulfate, potassium hydroxide, potassium dihydrogen phosphate and distilled water; and the reagent B consists of potassium chloride, sodium chloride, sodium phosphate and distilled water. The method includes: 1. taking mature female nematodes of the same species into the reagent A of the living nematoda egg purification and extraction agent, conducting centrifugation after shaking, discarding the supernatant and reserving the precipitate; 2. adding the reagent B of the living nematoda egg purification and extraction agent into the precipitate, carrying out centrifugation and taking the sediment. With the invention, the nematode consumption can be reduced by 50%, the egg obtaining rate can be enhanced by 30% on average, the nematode egg germination rate can be improved by over 20% on average; the egg obtaining rate can be up to 60% of natural reproduction; and the egg hatching rate can reach 92.6%.

Description

technical field [0001] The present invention relates to extractants and methods for their use. Background technique [0002] Nematodes belong to the phylum Nematoda, with 15,000 known species, the largest number of animal groups on earth. Nematodes have a wide range of habitats, complex nutritional structures, short life cycle, simple and efficient isolation methods, and easy classification and identification. They have been extensively and in-depth researched in the field of molecular biology. Because of their advantages and potential as indicator organisms, they can be used in agronomy and The field of environmental ecology is applied as a biomarker. Nematodes are used as model organisms and indoor simulation experiments require the maintenance of provenance, subculture and co-culture of the target nematodes. [0003] As the basis of nematode laboratory research, the method of obtaining sufficient and high-quality nematode eggs for controlled culture has been continuousl...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/07
Inventor 王云彪武海涛吴东辉
Owner NORTHEAST INST OF GEOGRAPHY & AGRIECOLOGY C A S
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