Method for quantification of total phosphorous

Abstract

The purpose is to quantify the total phosphorous in water of interest safely and within a short time. Provided is a method for quantifying the total phosphorus in water of interest by decomposing phosphorous compounds contained in the water to convert the phosphorous compounds into phosphate ions and determining the quantity of the phosphate ions in the water, wherein an alkali metal salt of peroxydisulfuric acid and sulfuric acid are added to the water and the resulting mixture is heated at a temperature ranging from 65°C to a boiling point for a predetermined period. Subsequently, a color-developing agent comprising at least one hydroxy-group-containing compound selected from a group of hydroxy-group-containing compounds consisting of hydroxycarboxylic acids and alditols, a saccharide compound selected from a group of saccharide compounds consisting of aldoses having 5 carbon atoms, aldoses having 6 carbon atoms, ketoses having 6 carbon atoms and monosaccharide-generating compounds capable of being decomposed to generate any one of the aforementioned monosaccharides and hexaammonium heptamolybdate is added to the water, the resulting mixture is heated at 65°C or higher for a predetermined period, and the absorbance of any wavelength ranging from 600 to 950 nm in the water is measured.

Description

technical field

[0001] The present invention relates to a method for quantifying total phosphorus, in particular, after decomposing phosphorus compounds contained in test water into phosphate ions, the phosphate ions in the test water are quantified, and the total phosphorus in the test water is thus quantified. Quantitative method. Background technique

[0002] Phosphorus is one of the causative substances related to eutrophication of ocean water, lake water, river water, groundwater, etc. Therefore, discharge restrictions are set in factory effluents, etc., and phosphate ion quantification is required before discharge of factory effluents, etc. into the environment. Here, factory effluents and the like contain phosphorus not only as phosphate ions but also as various phosphorus compounds, which become sources of phosphorus by natural decomposition after they are discharged into the environment. Therefore, it is sometimes required to quantify not only phosphate ions but al...

Examples

Embodiment

[0083] unit

[0084] The unit of mg[P] / L represents the number of micrograms of phosphorus contained in 1L of water.

[0085] Reagents and Spectrophotometers

[0086] Reagents and spectrophotometers used in the following Examples and the like are as follows.

[0087] Phosphorus standard solution (for water quality testing): Wako Pure Chemical Industries, Ltd. No. 160-19241

[0088] Silicon standard solution: Wako Pure Chemical Industries, Ltd. No. 192-06031

[0089] 1M sulfuric acid (for capacity analysis): Wako Pure Chemical Industries Ltd. No. 198-09595

[0090] Hydrochloric acid (special grade reagent): Wako Pure Chemical Industries Co., Ltd. No. 080-01066

[0091] Potassium peroxodisulfate (for the determination of nitrogen and phosphorus): Wako Pure Chemical Industries, Ltd. No. 169-1189

[0092] Ammonium peroxodisulfate (special grade reagent): Wako Pure Chemical Industries Co., Ltd. No. 012-03285

[0093] Hexaammonium heptamolybdate tetrahydrate (special grade...

experiment example 1

[0132] The following sample water and oxidizing agent aqueous solution were prepared. The D-glucose used in the preparation of the sample waters 2, 3, and 4 was assumed to be an organic substance as a mixture.

[0133]

[0134] Sample water 1:

[0135] Phosphate ion solution with a phosphate ion concentration of 5 mg[P] / L.

[0136] Sample water 2:

[0137] An aqueous solution obtained by dissolving sodium diphosphate decahydrate and D-glucose in distilled water (sodium diphosphate conversion concentration: 5 mg[P] / L, D-glucose concentration: 40 mg / L).

[0138] Sample water 3:

[0139] An aqueous solution obtained by dissolving adenosine-5'-triphosphate disodium trihydrate and D-glucose in distilled water (concentration in terms of adenosine-5'-triphosphate disodium is 5 mg[P] / L, D-glucose concentration is 40mg / L).

[0140] Sample water 4:

[0141] An aqueous solution obtained by dissolving disodium phenyl phosphate dihydrate and D-glucose in distilled water (concentrat...

experiment example 2

[0160] Prepare the following sample water. The D-glucose used in the preparation of the sample waters 5 and 6 is assumed to be an organic substance which is a mixture.

[0161] Sample water 5:

[0162] Adenosine-5'-triphosphate disodium trihydrate was prepared by dissolving adenosine-5'-triphosphate disodium trihydrate, phenyl phosphate disodium dihydrate and D-glucose in distilled water to a concentration of 1 mg [P ] / L, an aqueous solution with a concentration of disodium phenyl phosphate of 1 mg[P] / L and a concentration of D-glucose of 50 mg / L.

[0163] Sample water 6:

[0164] An aqueous solution having a sodium diphosphate concentration of 2 mg[P] / L and a D-glucose concentration of 50 mg / L was prepared by dissolving sodium diphosphate decahydrate and D-glucose in distilled water.

[0165] To 5 mL of each sample water, 0.8 mL of an aqueous potassium peroxodisulfate solution having a concentration of 30 g / L and 1.2 mL of 1M sulfuric acid were added. Then, each sample wa...

technical field

[0001] The present invention relates to a method for quantifying total phosphorus, in particular, after decomposing phosphorus compounds contained in test water into phosphate ions, the phosphate ions in the test water are quantified, and the total phosphorus in the test water is thus quantified. Quantitative method. Background technique

[0002] Phosphorus is one of the causative substances related to eutrophication of ocean water, lake water, river water, groundwater, etc. Therefore, discharge restrictions are set in factory effluents, etc., and phosphate ion quantification is required before discharge of factory effluents, etc. into the environment. Here, factory effluents and the like contain phosphorus not only as phosphate ions but also as various phosphorus compounds, which become sources of phosphorus by natural decomposition after they are discharged into the environment. Therefore, it is sometimes required to quantify not only phosphate ions but al...

Examples

Embodiment

[0083] unit

[0084] The unit of mg[P] / L represents the number of micrograms of phosphorus contained in 1L of water.

[0085] Reagents and Spectrophotometers

[0086] Reagents and spectrophotometers used in the following Examples and the like are as follows.

[0087] Phosphorus standard solution (for water quality testing): Wako Pure Chemical Industries, Ltd. No. 160-19241

[0088] Silicon standard solution: Wako Pure Chemical Industries, Ltd. No. 192-06031

[0089] 1M sulfuric acid (for capacity analysis): Wako Pure Chemical Industries Ltd. No. 198-09595

[0090] Hydrochloric acid (special grade reagent): Wako Pure Chemical Industries Co., Ltd. No. 080-01066

[0091] Potassium peroxodisulfate (for the determination of nitrogen and phosphorus): Wako Pure Chemical Industries, Ltd. No. 169-1189

[0092] Ammonium peroxodisulfate (special grade reagent): Wako Pure Chemical Industries Co., Ltd. No. 012-03285

[0093] Hexaammonium heptamolybdate tetrahydrate (special grade...

experiment example 1

[0132] The following sample water and oxidizing agent aqueous solution were prepared. The D-glucose used in the preparation of the sample waters 2, 3, and 4 was assumed to be an organic substance as a mixture.

[0133]

[0134] Sample water 1:

[0135] Phosphate ion solution with a phosphate ion concentration of 5 mg[P] / L.

[0136] Sample water 2:

[0137] An aqueous solution obtained by dissolving sodium diphosphate decahydrate and D-glucose in distilled water (sodium diphosphate conversion concentration: 5 mg[P] / L, D-glucose concentration: 40 mg / L).

[0138] Sample water 3:

[0139] An aqueous solution obtained by dissolving adenosine-5'-triphosphate disodium trihydrate and D-glucose in distilled water (concentration in terms of adenosine-5'-triphosphate disodium is 5 mg[P] / L, D-glucose concentration is 40mg / L).

[0140] Sample water 4:

[0141] An aqueous solution obtained by dissolving disodium phenyl phosphate dihydrate and D-glucose in distilled water (concentrat...

experiment example 2

[0160] Prepare the following sample water. The D-glucose used in the preparation of the sample waters 5 and 6 is assumed to be an organic substance which is a mixture.

[0161] Sample water 5:

[0162] Adenosine-5'-triphosphate disodium trihydrate was prepared by dissolving adenosine-5'-triphosphate disodium trihydrate, phenyl phosphate disodium dihydrate and D-glucose in distilled water to a concentration of 1 mg [P ] / L, an aqueous solution with a concentration of disodium phenyl phosphate of 1 mg[P] / L and a concentration of D-glucose of 50 mg / L.

[0163] Sample water 6:

[0164] An aqueous solution having a sodium diphosphate concentration of 2 mg[P] / L and a D-glucose concentration of 50 mg / L was prepared by dissolving sodium diphosphate decahydrate and D-glucose in distilled water.

[0165] To 5 mL of each sample water, 0.8 mL of an aqueous potassium peroxodisulfate solution having a concentration of 30 g / L and 1.2 mL of 1M sulfuric acid were added. Then, each sample wa...

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