High-temperature resistant yeast and application thereof

A technology of high temperature resistance and yeast, applied in the field of microorganisms

Inactive Publication Date: 2012-06-13
KUNMING UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The object of the present invention is to overcome the shortcoming of tra

Method used

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  • High-temperature resistant yeast and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0058] Embodiment 1: high temperature resistant yeast Ogataea For the screening of sp. WXT3 strain, the specific operation process is as follows:

[0059] Take 5g of soil sample from Xishuangbanna orchard, inoculate it into 100mL YPD liquid medium, add chloramphenicol and tetracycline at a final concentration of 50μg / ml to the medium, and culture it on a shaker at 42°C and 150rpm for 2 days; take the culture 100μl, inoculated into 10ml of fresh YPD liquid medium, 24 days later, take 100μl of the culture and spread it on the YPD agar plate, incubate at 42°C for 24h, pick a single colony and inoculate it on the YPD liquid agar medium, a total of 26 single colonies were selected , cultured at 45°C and 48°C, and the colony with the highest ethanol yield was selected as WXT3, and then identified by molecular biology, physiology and biochemistry, and sent to CCTCC for preservation. The preservation number is: CCTCC NO: M 2011108.

[0060] The culture medium used in this embodimen...

Embodiment 2

[0064] Embodiment 2: high temperature resistant yeast Ogataea Sp. WXT3 Strain Utilizes Glucose Fermentation to Produce Ethanol at Different Temperatures

[0065] Take a 250ml Erlenmeyer flask, add 100ml of culture medium (medium composition: 5% glucose, 1% yeast extract, 1% peptone), sterilize at 115°C for 20min; prepare YPD liquid medium at the same time, inoculate Ogataea sp. WXT3 yeast strain, cultivated at 42°C for 8 hours, and used as seed culture solution for later use.

[0066] Add 5mL seed culture (5% (V / V) for fermentation inoculum) into a sterilized Erlenmeyer flask, and ferment at 30°C, 37°C, 42°C, 45°C, 150rpm shaking table respectively, and set up three groups in parallel Fermentation, after 72 hours of fermentation, the ethanol concentrations in the system produced by fermentation at 30°C, 37°C, 42°C, and 45°C were 15.41g / L, 13.41g / L, 13.45g / L, and 1.34g / L, respectively. The results See Figure 7 . The ethanol yield when fermented at 42°C was the same as ...

Embodiment 3

[0068] Embodiment 3: high temperature resistant yeast Ogataea Sp. WXT3 strains use different concentrations of glucose to ferment and produce ethanol

[0069] Take a 250ml Erlenmeyer flask, add 100ml medium (medium composition: 10% or 15% glucose, 0.5% yeast extract, 1% peptone), sterilize at 115°C for 20min, prepare YPD liquid medium at the same time, and inoculate WXT3 yeast strain , cultivated at 42°C for 8h, and used as seed culture for later use.

[0070] Add 5mL seed culture into a sterilized Erlenmeyer flask, ferment and culture on a shaker at 42°C and 150rpm, set up three groups of parallel fermentation, the results are shown in Figure 8 , the figure shows that before 72h, the concentration of ethanol increases with the prolongation of fermentation time, and the concentration of ethanol decreases with the increase of glucose concentration. From 72h to 96h, the concentration of ethanol fermented with 10% glucose is lower than the concentration of ethanol fermented ...

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Abstract

The invention discloses high-temperature resistant yeast and application thereof. The high-temperature resistant yeast Ogataeasp.WXT3 is preserved in China Center for Type Culture Collection with the preservation number of CCTCC NO:M2011108; the yeast disclosed by the invention is screened out from soil, the strain can be fermented at the temperature of 25-48 DEG C to produce ethanol; and ethanolyield is high, carbon sources which can be used for fermentation are wide, the strain is especially applicable to an ethanol preparation process by virtue of a lignocelulose simultaneous saccharification and fermentation (SSF) process, and usage amount of cellulose can be effectively reduced, thus production cost of cellulosic ethanol is reduced.

Description

technical field [0001] The invention relates to a strain of high-temperature-resistant yeast and its application, belonging to the technical field of microbes. Background technique [0002] At present, industrially, yeast is fermented at 28-33°C to produce ethanol. Due to the heat production of yeast fermentation, the temperature of the fermentation system rises, causing changes in the fatty acids, phospholipids, ergosterol and other components in the yeast cells, affecting the normal physiological activities of the cells themselves (Araque Edgardo, Parra Carolinaa, Rodr?guez Manuel, Freer Juanita, Baeza Jaime. Selection of thermotolerant yeast strains Saccharomyces cerevisiae for bioethanol production [J]. Enzyme and Microbial Technology, 2008, 43: 120-123.). Therefore, in order to maintain the activity of yeast, it is necessary to use a large amount of cooling water to reduce the fermentation temperature. At the same time, in order to prevent contamination by bacteria, ...

Claims

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Application Information

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IPC IPC(8): C12N1/16C12P7/06C12P7/10C12R1/645
CPCY02E50/17Y02E50/16Y02E50/10
Inventor 伊日布斯王乔平严金平张绪
Owner KUNMING UNIV OF SCI & TECH
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