Hydrogen peroxide biosensor and preparation method thereof

A biosensor, hydrogen peroxide technology, applied in instruments, scientific instruments, electrochemical variables of materials, etc., to achieve the effects of no toxic side effects, simple operation, and good response

Inactive Publication Date: 2012-06-13
SHANGHAI NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] So far, there is no biosensor for detecting hydrogen peroxide prepared by "green economy" calcium phytate membrane at home and abroad.

Method used

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  • Hydrogen peroxide biosensor and preparation method thereof
  • Hydrogen peroxide biosensor and preparation method thereof
  • Hydrogen peroxide biosensor and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0028] The electrochemical experiment of this embodiment was carried out on the CHI 660D electrochemical workstation (Shanghai Chenhua Instrument Co., Ltd.); the ultraviolet-visible (UV-vis) spectrum experiment used the UV-8500 ultraviolet-visible spectrophotometer (Shanghai Tianmei Scientific Instrument Co., Ltd.) Co., Ltd.); other instruments are FE20 laboratory pH meter (Mettler-Toledo Instruments Shanghai Co., Ltd.); SK2200H ultrasonic instrument (Shanghai Kedao Ultrasonic Instrument Co., Ltd.).

[0029] The preparation method of hydrogen peroxide biosensor of the present invention is as follows:

[0030] (1) Heating 0.001mol / L calcium phytate solution to reflux at 90°C for 30 minutes;

[0031] (2) Pipette 4 μL of the above calcium phytate solution and drop it onto the surface of the pretreated glassy carbon electrode (7mm 2 ), dried in a refrigerator at 4°C to obtain modified calcium phytate (Ca-IP 6 ) film glassy carbon electrode (Ca-IP 6 / GCE);

[0032] (3) Take 4 μ...

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Abstract

The invention discloses a hydrogen peroxide biosensor and a preparation method thereof. According to the invention, by using a self-assembly process, a Nafion / HRP / Ca-IP6 / GCE sensor is prepared by absorbing HRP onto the surface of an electrode through electrostatic interaction and chelation between phosphate ester bonds and Ca<2+>, with calcium phytate as a bridging agent; the prepared sensor has high affinity to an enzyme substrate, can realize direct electron transfer of horseradish peroxidase and has performance like a short response time to hydrogen peroxide, low detection limit and high sensitivity. Meanwhile, the preparation method provided in the invention is simple, environment-friendly and economy.

Description

technical field [0001] The invention belongs to the field of biological materials, and in particular relates to a hydrogen peroxide biosensor and a preparation method thereof. Background technique [0002] h 2 o 2 As an important chemical product, it has multiple functions such as sterilization, bleaching, oxidation and disinfection. Its application is very extensive, so for H 2 o 2 The determination of content is of great significance in the fields of biopharmaceuticals, food science, industrial analysis, environmental monitoring and clinical chemistry. Now detect H 2 o 2 The main methods used are fluorescence method, chemiluminescence method, electrochemical method, spectroscopic method and titration method. In recent years, electrochemical methods, especially direct electrochemical media-free biosensors based on enzymes, have been developed rapidly, because the electrochemically developed H 2 o 2 Biosensors are simple to prepare, low in cost, and have high select...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/30G01N27/327
Inventor 杨海峰接德丽
Owner SHANGHAI NORMAL UNIVERSITY
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