Test tube breeding method of lilium oriental hybrid seed ball

Abstract

The invention discloses a test tube breeding method of a lilium oriental hybrid seed ball, and relates to a flowering plant tissue culture seedling method. The test tube breeding method comprises the steps of scale selection and disinfection, scale induction, bulblet propagation and test tube balling. The scale induction is completed as follows: cutting and taking the bases of scales, inoculating the stem bases on an induction culture medium of MS (Murashige and Skoog medium) + 6-BA (6-benzylaminopurine) 2mg/L + NAA (naphthalene acetic acid) 0.2mg/L + sucrose 40g/L + agar 7g/L; conducting propagation for 30-40d, then inoculating the bulblet in an enrichment medium of MS+6-BA 2mg/L + NAA 0.2mg/L + sucrose 40g/L + agar 7g/L for propagation; and after two months, inoculating the bulblet in a balling-up culture medium (pH 5.8) of MS + 6-BA 0.2mg/L + NAA 0.5mg/L + CCC (chlormequat chloride) 150mg/L + sucrose 80g/L + agar 7g/L, with illumination density of 2,000-2,200lx, 14h/d, and temperature of 23-25 DEG C until the balling-up is completed. The test tube breeding method of lilium oriental hybrid seedball has the following advantages: the diameter and the raw weight of the seed ball are increased obviously, the transplanting survival rate of the seed ball is increased, and the breeding period of the commodity seed ball of the lilium oriental hybrid seed ball is shortened, so that the production cost is lowered.

Description

Technical field: [0001] The invention relates to a flower tissue culture seedling method, in particular to the test tube breeding of oriental lily bulbs. Background technique: [0002] Oriental lily is a perennial bulbous flower of the genus Lilium in the family Liliaceae. It has large and beautiful flowers, rich colors, and strong fragrance. It has high ornamental value and economic value. It is one of the four major cut flowers in the world. The proportion of oriental lily in my country's cut flower industry is increasing, but most of the bulbs still rely on imports, and the quality is uneven, which seriously restricts the development of fresh cut lily in my country. High-quality virus-free bulbs can be produced by using tissue culture technology combined with shoot tip detoxification. However, the domestic production of lily bulbs in my country is still progressing slowly. The existing bulb breeding technology generally produces lily seedlings through tissue culture first...

AI Technical Summary

Problems solved by technology

[0014] Shortcomings of the prior art: 1. The transplanting survival rate of lily tissue-cultured seedlings is low; 2. Because the bulbs of tissue-cultured seedlings are small, the cycle of breeding in the field is long; 3. The production cost is high, and the breeding process is easily affected by the natural environment