Method for detecting protein and metal ions
A technology of metal ions and proteins, applied in the field of detection of proteins and metal ions, can solve the problems of unfavorable promotion and high detection cost, and achieve the effects of low price, excellent detection performance and simple preparation method
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Embodiment 1
[0034] Embodiment 1: the preparation of the nanorod of polym-phenylenediamine (PMPD) and detection protein
[0035] Material preparation and processing: at room temperature, mix 0.06mL of 0.5M ammonium persulfate aqueous solution with 0.84mL of water, then add 0.1mL of 0.1M m-phenylenediamine aqueous solution and shake, and then a large amount of polym-phenylenediamine can be seen Precipitation of nanospheres of amine occurs. The resulting precipitate was washed with secondary water and centrifuged, and the operation was repeated several times, and then dispersed in water to prepare poly-m-phenylenediamine (PMPD) nanorods, which were stored at 4°C for future use.
[0036] Detection of protein: the fluorescent probe (T A ), with human thrombin protein as the target protein for detection (T B ). Detection method: first add PMPD nanorods to the fluorescent probe (50nM T A ) solution, single-stranded DNA probes adsorb to the surface of PMPD nanorods, causing fluorescence quenc...
Embodiment 2
[0037] Example 2: nano-C 60 Preparation and detection of protein and metal ions
[0038] Material preparation and handling: 2.5 mg of C 60 Dissolve it in 2mL of acetone, then add 12mL of acetonitrile dropwise while stirring, and then a large amount of khaki precipitate appears. Wash the precipitate with acetonitrile and centrifuge, repeat the operation several times, and then sonicate for 30 minutes to make the obtained Nano-C 60 Evenly dispersed in 8mL of water for later use.
[0039] Detection of protein: the single-stranded DNA sequence modified by fluorophore (FAM) with specific recognition ability to human thrombin protein is used as a fluorescent probe for fluorescent detection protein (T A ), with human thrombin protein as the target protein for detection (T B ). First the nano-C 60 Added to the fluorescent probe (50nMT A ) solution, single-stranded DNA probes will be adsorbed to nano-C 60 surface, causing fluorescence quenching. After a certain reaction time (...
Embodiment 3
[0041] Example 3: Preparation of mesoporous silicon and detection of proteins and metal ions
[0042] Material preparation and treatment: Add 1 g of mesoporous silicon (MS) to 5 g of water, then add 1.25 g of glucose and 0.14 g of 98% H 2 SO 4 , after stirring at room temperature for 5 h, the resulting mixture was reacted at 100° C. and 160° C. for 6 h, respectively. Afterwards, the reaction mixture was added to 5 g of water, followed by the addition of 0.75 g of sugar and 0.09 g of 98% H 2 SO 4 . After stirring for 12 h, the reaction mixture was dried at 160 °C for 6 h. Finally, at 800°C N 2 Calcined under protected conditions for 4h to complete the carbonization process. The obtained carbon-silicon composite was washed with 1M NaOH (water / ethanol mixture) for 12 h to remove the silicon template, and then dried at room temperature to obtain mesoporous carbon (MC) particles.
[0043] Detection of protein: the single-stranded DNA sequence modified by fluorophore (FAM) wi...
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