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Preparation method of dye nanoparticle with aqueous phase dispersion and adjustable wavelength

A nanoparticle and dispersion technology, applied in the field of preparation and surface hydrophilic modification of nanoparticles, to achieve mild reaction process, high yield and good repeatability

Inactive Publication Date: 2012-06-27
SUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] In addition, although the inorganic quantum dot fluorescent probes developed in recent years have the advantages of high brightness, high photostability, and large Stokes shift, the problem of their own potential toxicity has been difficult to avoid (Hoshino, A.; Fujioka, K.; Oku, T.; Suga, M.; Sasaki, Y. F.; Ohta, T.; Yasuhara, M.; Suzuki, K.; Yamamoto, K. Nano Lett. 2004, 4, 2163-2169)

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] A method for preparing aqueous dispersion wavelength-tunable dye nanoparticles, which comprises the following steps:

[0038] Step 1) Prepare two hydrophobic organic fluorescent dye molecules into doped nanoparticles by solvent exchange method; the specific method is to mix the two organic fluorescent dye molecules according to the molar ratio of 20:0.05-20:4 Prepare a mixed fluorescent dye molecular solution in a good solvent, take an appropriate volume of the organic fluorescent dye molecular mixed solution and add it to an appropriate amount of poor solvent, stir magnetically for 3 minutes and then let stand for 6 hours to obtain doped fluorescent nanoparticles;

[0039] Step 2) Take the amphiphilic surfactant and configure it into a 1 mg / mL amphiphilic surfactant aqueous solution; add 200 μL of the 1 mg / mL amphiphilic surfactant aqueous solution to the suspension of the precursor nanoparticles, and ultrasonically After dispersing for 5 minutes and standing still for...

Embodiment 2

[0052] In this example, three kinds of hydrophobic organic fluorescent dye molecules were used to prepare doped nanoparticles, and the molar ratio of the three small organic dye molecules was 20:0.25:0.05-20:0.25:2. Other steps are with embodiment 1.

[0053] Preferably, the molar ratio of the three hydrophobic organic fluorescent dye molecules is 20:0.25:0.5

Embodiment 3

[0055] The present invention will be further described by taking the hydrophobic organic fluorescent dye molecule 2-tert-butyl-9,10-di(2-naphthyl)anthracene and coumarin system as an example:

[0056] Firstly, two small organic dye molecules were mixed and dissolved in tetrahydrofuran solvent with a molar ratio of 20:0.05.

[0057] Secondly, uniformly dispersed 2-tert-butyl-9,10-di(2-naphthyl)anthracene-coumarin-doped fluorescent nanoparticles were obtained by solvent exchange method, with an average kinetic diameter of 98nm.

[0058] Finally, 200 μL of 1 mg / mL amphiphilic surfactant was added to 60 μmol of 2-tert-butyl-9,10-bis(2-naphthyl)anthracene-coumarin-doped fluorescent nanoparticles solution, and ultrasonically dispersed for 5 minutes , standing still for 6 hours, prepared comfortable fluorescent nanoparticles with an average kinetic particle diameter of 110nm dispersed in the aqueous phase, which has high stability in physiological saline, and the kinetic diameter rem...

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Abstract

The invention discloses a preparation method of a dye nanoparticle with aqueous phase dispersion and adjustable wavelength, which comprises the following steps: (1) mixing more than one hydrophobic organic fluorescent dye molecule through a solvent exchanging method to prepare a doped nanoparticle; and (2) preparing amphipathic nature surface active agent into 1mg / ml of amphipathic nature aqueous surfactant solution; and adding 200mul 1mg / ml of amphipathic nature aqueous surfactant solution into suspension liquid of the doped nanoparticle, carrying out ultrasonic dispersion for 5 minutes, and standing for 6 hours. According to the preparation method disclosed by the invention, the proportion of two or three organic dye molecules is controlled, so that emission wavelength of the doped fluorescent dye particle with aqueous phase dispersion and hydrophobicity from blue light to green light and red light can be continuously adjusted, and the luminous intensity is controllable. The method is simple and easy to realize, and can be widely applied to fields of biology, medical science, chemistry and the like.

Description

technical field [0001] The invention relates to the preparation of hydrophobic organic dye-doped nanoparticles and the hydrophilic modification of the surface of the nanoparticles, in particular to a method for preparing dye nanoparticles with adjustable wavelength in aqueous phase dispersion based on doping technology. [0002] Background technique [0003] Organic dyes are widely used in the field of biological imaging. However, they face many problems, such as easy aggregation of hydrophobic organic dyes in aqueous solution, low photostability, small Stokes shift, etc., which greatly restrict their application. limited. Therefore, in recent years, a large number of improved bioluminescent probes based on organic dyes have emerged, mainly including: composite silicon sphere structures formed by dyes connected to silicon substrates through physical adsorption or chemical bonds; dye-doped polymer nanostructures . In these systems, since the direct contact between the dye ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/06C09B67/22
Inventor 张秀娟刁晓军俞佳张晓宏
Owner SUZHOU UNIV
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