Preparation of water soluble saccharide fluorescent molecular probe and rapid detection for iron ions in water and biological tissue

Abstract

The invention provides a preparation of a water soluble saccharide fluorescent molecular probe as well as a direct and rapid detection for iron ions in water and a biological tissue. The fluorescent molecular probe obtains very high selectivity and sensitivity for iron ions in water and serum; after the fluorescent molecular probe is selectively combined with the iron ions, the color change of the solution from yellow to red brown can be observed by naked eyes; and the lower detection limit can reach 10-6 M. Without need of instruments, the field real-time direct and rapid detection can be carried out. The fluorescent molecular probe can be prepared to obtain an iron ion detecting test paper and a detecting kit and can be also coated with films to obtain a portable solid detector by using viscosity of saccharide compounds. The specific structure of the saccharide fluorescent molecular probe (taking a glucosamine ligand for example): except for glucosamine, the saccharide ligand can be also other saccharide compounds and derivatives thereof.

Description

technical field [0001] The invention relates to the preparation of a class of water-soluble carbohydrate fluorescent molecular probes and the rapid detection of iron ions in water and biological tissues. Background technique [0002] Iron ion is one of the ions necessary for living organisms. It is a necessary substance for the manufacture of heme and myoglobin and the promotion of vitamin B metabolism. Many biological and chemical processes at the cellular level require the participation of iron ions, so the research can be used for iron ions The detected fluorescent molecular probes are of great significance to the environment and life sciences. It is reported in the literature that the synthesis method of fluorescent molecular probes for iron ions is complex and costly. The detection of iron ions by the synthesized products is outside the visible spectrum range, and the color change cannot be observed with the naked eye, and expensive detection instruments must be used. ...

AI Technical Summary

Problems solved by technology

It has been reported in the literature that the synthesis method of fluorescent molecular probes for iron ions is complex and costly. The detection of iron ions by the synthesized products is outside the visible spectrum range, and the color change cannot be observed by the naked eye, and expensive detection instruments must be used.

In addition, there are still disadvantages such as sample pretreatment, insufficient detection speed, poor water solubility, and expensive detection.

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