Alcaligenes denitrificans with denitrification performance and application thereof
A denitrifying alcaligenes and denitrification technology, applied in the direction of bacteria, chemical instruments and methods, and methods based on microorganisms, can solve the rare and undiscovered problems of degrading nitrate, and achieve the effect of strong tolerance
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Embodiment 1
[0019] Example 1: Isolation, purification and identification of Alcaligenes denitrificans TB
[0020] 1. Isolation and purification of Alcaligenes denitrificans TB
[0021] Alcaligenes denitrificans TB is a Gram-negative bacterium induced by denitrifying bacteria screened from the activated sludge of Qige Wastewater Treatment Plant in Hangzhou, Zhejiang Province. Specific steps are as follows:
[0022] The denitrifying bacteria sieved from the activated sludge of Qige Wastewater Treatment Plant in Hangzhou City, Zhejiang Province were taken. Make bacterial suspension from the sieved strains, transfer 3mL of bacterial suspension into a sterile petri dish with a diameter of 9cm, put a sterile magnetic rotor on a magnetic stirrer, and irradiate at 30cm under a 30W ultraviolet lamp for 1min . Under the infrared light, 0.1 mL of the irradiated bacterial solution was taken and spread on the plate medium, and the plate was wrapped with a black cloth, and incubated at a constant te...
Embodiment 2
[0032] Example 2: Detection of denitrification performance of Alcaligenes denitrificans TB
[0033] 1. Investigate the denitrification performance of bacteria Alcaligenes denitrificans TB under different initial pH conditions
[0034] The Alcaligenes denitrificans TB denitrification experiment was carried out at different initial pHs, and it was found that it has a high denitrification ability at pH9. From the perspective of practical application, pH9 is the best pH, and the degradation rate is the highest at this time. The specific implementation steps are as follows:
[0035] With nitrate as the sole nitrogen source (NO 3 - Concentration 400mg·L -1 ), add D600 of 0.4 bacterial solution to the culture medium (sodium succinate 3050mg L by 1% inoculum -1 , KH 2 PO 4 1000mg·L -1 , K 2 HPO 4 1000mg·L -1 , MgSO 4 .7H 2 O 200mg·L -1 , CuSO 4 1mg·L -1 , FeSO 4 1mg·L -1 , MnSO 4 5mg·L -1 , Na 2 MoO 4 1mg·L -1 , ZnCl 2 2mg·L -1 , NaNO 3 550mg·L -1 , the solven...
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