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Achromobacter xylosoxidans with denitrification and dephosphorization function and application of Achromobacter xylosoxidans

A colorless bacillus and xylose technology, applied in the direction of bacteria, aerobic treatment, chemical instruments and methods, etc., can solve the basic problems of single denitrification or phosphorus removal function research, and achieve slow value-added and high cell yield , the effect of fast growth rate

Active Publication Date: 2012-07-04
PEKING UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Both the theory of heterooxynitrification-aerobic denitrification biological denitrification and the theory of PAOs / DPB biological phosphorus removal are based on the study of a single denitrification or phosphorus removal function. So far, no research has shown that a microorganism has both different functions. Functions of Oxynitrification-Aerobic Denitrification in Nitrogen Removal and Phosphorus Removal

Method used

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  • Achromobacter xylosoxidans with denitrification and dephosphorization function and application of Achromobacter xylosoxidans
  • Achromobacter xylosoxidans with denitrification and dephosphorization function and application of Achromobacter xylosoxidans
  • Achromobacter xylosoxidans with denitrification and dephosphorization function and application of Achromobacter xylosoxidans

Examples

Experimental program
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Effect test

Embodiment 1

[0032] Implementation example 1: Determination of phosphorus removal ability of Achromobacter xylosoxidans under aerobic conditions

[0033] The Achromobacter xylosoxidans strain (preserved in the General Microbiology Center of the China Microbial Culture Collection Management Committee (CGMCC) on March 19, 2009, the preservation number is CGMCC № 2964, the same below) was inoculated in 1L In the LB medium, prevent the invasion of bacteria and maintain the growth vigor of the bacteria, and carry out enrichment culture. Centrifuge the cultured bacterial solution and wash three times with sterile water to make the optical density (OD 600 ) Is a 1-2 bacterial suspension.

[0034] Take 5mL of the above bacterial suspension and add two test media containing 120mL (each L contains 5g sodium succinate, 0.1911g NH 4 Cl, 0.044g KH 2 PO 4 , 0.2g CaCl 2 ·2H 2 O, 0.5g MgSO 4 ·7H 2 O, 0.006g FeSO 4 ·7H 2 O, pH 7.0-7.5) Erlenmeyer flask, sealed with 9 layers of gauze, shaking culture in a shaker...

Embodiment 2

[0036] Implementation example 2: Determination of the optimal phosphorus removal conditions of Achromobacter xylosoxidans

[0037] Using the model of shake flask experiment, based on the principle of orthogonal experiment, with four factors of pH value, shaker culture temperature, shaker speed and carbon-nitrogen ratio as influencing factors, a total of 9 orthogonal experiments with 4 factors and 3 levels were established to determine The optimal phosphorus removal conditions for the strain Achromobacter xylosoxidans. The medium selected in the experiment except that the content of sodium succinate was changed as needed, and the remaining components and concentrations were the same as in Example 1.

[0038] The orthogonal experimental design and results are shown in Table 1.

[0039] Table 1 Orthogonal experimental design and results

[0040]

[0041] Through the analysis of the results of the orthogonal experiment, it is found that the influence of the four factors on the phosphorus...

Embodiment 3

[0042] Implementation example 3: Simultaneous denitrification and dephosphorization capacity determination of Achromobacter xylosoxidans

[0043] The Achromobacter xylosoxidans strain was inoculated into 1L of LB medium to prevent the invasion of bacteria and maintain the growth vigor of the bacteria, and carry out enrichment culture. Centrifuge the cultured bacterial solution and wash three times with sterile water to make the optical density (OD 600 ) Is a 1-2 bacterial suspension.

[0044] Take 2.5mL of the above bacterial suspension and add two test media containing 120mL (each L containing 5g sodium succinate, 0.1911g NH 4 Cl, 0.044g KH 2 PO 4 , 0.2g CaCl 2 ·2H 2 O, 0.5g MgSO 4 ·7H 2 O, 0.006g FeSO 4 ·7H 2 O, pH 7.0-7.2) Erlenmeyer flask, sealed with 9 layers of gauze, shaking culture in a shaker at 30°C and 150 rpm. The medium without bacterial suspension was subjected to the experiment under the same conditions as a blank control. Take a small amount of the reaction solutio...

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Abstract

The invention relates to application of Achromobacter xylosoxidans with a heterotrophic nitrification-aerobic denitrification and dephosphorization function in wastewater treatment. The Achromobacter xylosoxidans can metabolize by using organic carbon as a unique carbon source and using ammonia nitrogen as a unique nitrogen source and can directly convert the ammonia nitrogen into gas products through the action of heterotrophic nitrification-aerobic denitrification so as to achieve the aim of denitrification, can also take nitrate nitrogen as a unique nitrogen source and convert the nitrate nitrogen into gas products through the action of aerobic denitrification, and can further ingest inorganic phosphorus and convert the inorganic phosphorus into a tissue of the Achromobacter xylosoxidans under an aerobic condition so as to achieve the aim of removing phosphorus from wastewater. The Achromobacter xylosoxidans are applied to the wastewater treatment, the simultaneous removal of nitrogen and phosphorus can be achieved under the single aerobic condition, and the difficult problem that the biological denitrification and dephosphorization in the traditional wastewater treatment need to adopt the segmented treatments of anaerobic phosphate release, anoxic denitrification and aerobic nitrification and phosphorus absorption, is better solved, so that the Achromobacter xylosoxidans have broad application prospects.

Description

Technical field [0001] The invention relates to a strain of Achromobacter xylosoxidans and its application in wastewater treatment. The strain has the function of heterotrophic nitrification-aerobic denitrification, and can simultaneously complete the removal of phosphorus-containing pollutants in sewage in the process of simultaneous nitrification and denitrification and denitrification, so as to achieve the purpose of simultaneous biological denitrification and phosphorus removal of domestic sewage. Background technique [0002] With the progress of society and economic development, eutrophication pollution of various water bodies in our country is becoming more and more serious, and the problem of cyanobacteria red tide is frequent, which has caused great damage to water safety and human health. The surplus of nutrients such as nitrogen and phosphorus is the root cause of eutrophication in water bodies. Eutrophication will occur when the concentration of N in the water body is...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C02F3/02C12R1/025
CPCY02W10/10
Inventor 陈倩倪晋仁邹仕强
Owner PEKING UNIV
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