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Achromobacter xylosoxidans with denitrification and dephosphorization function and application of Achromobacter xylosoxidans

An achromobacter, xylose technology, applied in bacteria, aerobic process treatment, chemical instruments and methods, etc., can solve the problem of single denitrification or phosphorus removal function research basis, etc., to solve the problem of slow value-added and high cell yield , the effect of fast growth rate

Active Publication Date: 2013-05-22
PEKING UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Both the theory of heterooxynitrification-aerobic denitrification biological denitrification and the theory of PAOs / DPB biological phosphorus removal are based on the study of a single denitrification or phosphorus removal function. So far, no research has shown that a microorganism has both different functions. Functions of Oxynitrification-Aerobic Denitrification in Nitrogen Removal and Phosphorus Removal

Method used

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  • Achromobacter xylosoxidans with denitrification and dephosphorization function and application of Achromobacter xylosoxidans
  • Achromobacter xylosoxidans with denitrification and dephosphorization function and application of Achromobacter xylosoxidans
  • Achromobacter xylosoxidans with denitrification and dephosphorization function and application of Achromobacter xylosoxidans

Examples

Experimental program
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Effect test

Embodiment 1

[0032] Implementation Example 1: Phosphorus removal capacity determination of Achromobacter xylosoxidans under aerobic conditions

[0033] Inoculate the Achromobacter xylosoxidans strain (preserved in the General Microbiology Center of China Committee for Microbial Culture Collection (CGMCC) on March 19, 2009, the preservation number is CGMCC№2964, the same below) in 1L In the LB medium, to prevent the invasion of miscellaneous bacteria and maintain the growth vigor of the bacteria, carry out enrichment culture. The cultured bacterial solution was centrifuged, washed three times with sterile water, and the optical density (OD 600 ) is the bacterial suspension of 1-2.

[0034] Take 5mL of the above bacterial suspension and add two test media containing 120mL (each L contains 5g sodium succinate, 0.1911gNH 4 Cl, 0.044g KH 2 PO 4 , 0.2g CaCl 2 2H 2 O, 0.5g MgSO 4 ·7H 2 O, 0.006g FeSO 4 ·7H 2 (0, pH7.0~7.5) Erlenmeyer flasks, sealed with 9 layers of gauze, and cultured i...

Embodiment 2

[0036] Implementation Example 2: Determination of Optimal Phosphorus Removal Conditions for Achromobacter xylosoxidans

[0037] Using the shaking flask experiment mode, according to the principle of orthogonal experiment, with pH value, shaker culture temperature, shaker speed and carbon-nitrogen ratio as the influencing factors, a total of 9 orthogonal experiments with 4 factors and 3 levels were established to determine Optimal phosphorus removal conditions for the strain Achromobacter xylosoxidans. The medium used in the experiment was the same as in Example 1 except that the content of sodium succinate was changed as needed.

[0038] Orthogonal experimental design and results are shown in Table 1.

[0039] Table 1 Orthogonal experiment design and results

[0040]

[0041] Through the analysis of the results of the orthogonal experiment, it was found that the strength of the influence of four factors on the phosphorus removal effect of the strain was: pH>carbon-nitroge...

Embodiment 3

[0042] Implementation example 3: Determination of simultaneous denitrification and phosphorus removal ability of Achromobacter xylosoxidans

[0043] The Achromobacter xylosoxidans strain was inoculated in 1 L of LB medium to prevent the invasion of miscellaneous bacteria and maintain the growth activity of the bacteria, and carry out enrichment culture. The cultured bacterial solution was centrifuged, washed three times with sterile water, and the optical density (OD 600 ) is the bacterial suspension of 1-2.

[0044] Take 2.5mL of the above bacterial suspension and add to two test media containing 120mL (each L contains 5g sodium succinate, 0.1911gNH 4 Cl, 0.044g KH 2 PO 4 , 0.2g CaCl 2 2H 2 O, 0.5g MgSO 4 ·7H 2 O, 0.006g FeSO 4 ·7H 2 (0, pH 7.0-7.2) Erlenmeyer flasks, sealed with 9 layers of gauze, and cultured in a shaker at 30° C. at 150 rpm. The culture medium that was not inoculated with the bacterial suspension was used as a blank control for experiments under ...

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Abstract

The invention relates to application of Achromobacter xylosoxidans with a heterotrophic nitrification-aerobic denitrification and dephosphorization function in wastewater treatment. The Achromobacter xylosoxidans can metabolize by using organic carbon as a unique carbon source and using ammonia nitrogen as a unique nitrogen source and can directly convert the ammonia nitrogen into gas products through the action of heterotrophic nitrification-aerobic denitrification so as to achieve the aim of denitrification, can also take nitrate nitrogen as a unique nitrogen source and convert the nitrate nitrogen into gas products through the action of aerobic denitrification, and can further ingest inorganic phosphorus and convert the inorganic phosphorus into a tissue of the Achromobacter xylosoxidans under an aerobic condition so as to achieve the aim of removing phosphorus from wastewater. The Achromobacter xylosoxidans are applied to the wastewater treatment, the simultaneous removal of nitrogen and phosphorus can be achieved under the single aerobic condition, and the difficult problem that the biological denitrification and dephosphorization in the traditional wastewater treatment need to adopt the segmented treatments of anaerobic phosphate release, anoxic denitrification and aerobic nitrification and phosphorus absorption, is better solved, so that the Achromobacter xylosoxidans have broad application prospects.

Description

technical field [0001] The invention relates to a strain of Achromobacter xylosoxidans and its application in waste water treatment. The strain has the function of heterotrophic nitrification-aerobic denitrification, and can simultaneously complete the removal of phosphorus-containing pollutants in sewage during the process of simultaneous nitrification and denitrification denitrification, thereby achieving the purpose of synchronous biological denitrification and phosphorus removal of domestic sewage. Background technique [0002] With the progress of the society and the development of the economy, the eutrophication pollution of various water bodies in our country is becoming more and more serious, and the problem of blue-green algae red tides occurs frequently, which has caused huge damage to the safety of water bodies and human health. The excess of nutrients such as nitrogen and phosphorus is the root cause of eutrophication in water bodies. When the concentration of N ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C02F3/02C12R1/025
CPCY02W10/10
Inventor 陈倩倪晋仁邹仕强
Owner PEKING UNIV
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