Preparation method for function feed additive capable of resisting bird flu

Abstract

A preparation method for a function feed additive capable of resisting bird flu comprises connecting parts of sections of artificially synthesized H5N1 bird flu virus hemagglutinin genes with eukaryotic expression vector protein bound idodine (pBI)-221 to obtain recombinant plasmid; converting the obtained recombinant plasmid in dunaliella through methods of particle guns, electric transform and the like to enable hemagglutinin to be expressed in the dunaliella; preparing to achieve engineering alga for expressing virus protein subunit; performing open expansion culture on the prepared engineering alga to collect alga bodies, and then obtaining the function feed additive capable of resisting bird flu through technological processes of low temperature drying, granulation and the like. The function feed additive prepared in the method is applied to poultry cultivation industries, has an immune protection function of resisting bird flu, can improve immune and growth speed poultry, has no side effects and is good in biological safety.

Description

technical field [0001] The invention relates to the field of feed additives, in particular to a preparation method of a functional feed additive for poultry with anti-avian influenza virus infection. Background technique [0002] Avian influenza disease is an acute and highly contagious disease of poultry caused by type A avian influenza virus, which can infect a variety of poultry, wild birds and mammals, and even spread to humans. At present, there is no really effective treatment method for bird flu disease, and the treatment methods for bird flu-infected animals are mainly culling and burning, which has brought losses and inconvenience to the entire affected area. Although vaccination has played a positive role in preventing and controlling the outbreak and prevalence of avian influenza, the rapid mutation of the avian influenza virus has caused immune failure or unsatisfactory effects. Moreover, the vaccines currently used in the market are mainly traditional vaccines,...

AI Technical Summary

Problems solved by technology

At present, there is no really effective treatment for bird flu disease, and the treatment methods for animals infected with bird flu are mainly culling and burning, which has brought losses and inconvenience to the entire affected area

Although vaccination has played a positive role in the prevention and control of bird flu outbreaks and epidemics, the rapid mutation of bird flu virus has caused immune failure or unsatisfactory effects

Moreover, the vaccines currently used in the market are mainly traditional vaccines, which are prepared by cultivating and inactivating live viruses, which have the disadvantages of high cost, cumbersome operation, pollution to the environment, and the possibility of dispersing the virus.

[0003] In actual production, it takes a long time for the immunization operation to prevent the occurrence of bird flu through vaccines. For example, it takes about 3 days to immunize 10,000 chicken farms. If the number of breeding is larger, the operation time will be longer

At the same time, the production and use costs of vaccines are relatively high, the investment in breeding is large, and the production costs are high.

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